1628052_at
type: EFO_0005072
part of: A-AFFY-35

CG11034
type: EnsemblDatabaseReference

1630590_at
type: EFO_0005072
part of: A-AFFY-35

Sr-CIV
type: EnsemblDatabaseReference

(Assay) control1m1
type: Assay
has participant: A-AFFY-35

E-GEOD-7614
type: microarray 1colour mrna differential
RO_0002162: Drosophila melanogaster
description: Alcoholism is a complex disorder determined by interactions between genetic and environmental risk factors. Drosophila represents a powerful model system to dissect the genetic architecture of alcohol sensitivity, as large numbers of flies can readily be reared in defined genetic backgrounds and under controlled environmental conditions. Furthermore, flies exposed to ethanol undergo physiological and behavioral changes that resemble human alcohol intoxication, including loss of postural control, sedation, and development of tolerance. We performed artificial selection for alcohol sensitivity for 25 generations and created duplicate selection lines that are either highly sensitive or resistant to ethanol exposure along with unselected control lines. We used whole genome expression analysis to identify 1,678 probe sets with different expression levels between the divergent lines, pooled across replicates, at a false discovery rate of q < 0.001. We assessed to what extent genes with altered transcriptional regulation might be causally associated with ethanol sensitivity by measuring alcohol sensitivity of 37 co-isogenic P-element insertional mutations in 35 candidate genes, and found that 32 of these mutants differed in sensitivity to ethanol exposure from their co-isogenic controls. Furthermore, 23 of these novel genes have human orthologues. Combining whole genome expression profiling with selection for genetically divergent lines is an effective approach for identifying candidate genes that affect complex traits, such as alcohol sensitivity. Because of evolutionary conservation of function, it is likely that human orthologues of genes affecting alcohol sensitivity in Drosophila may contribute to alcohol-associated phenotypes in humans. Experiment Overall Design: Starting with flies from Raleigh natural population (see material and methods) we performed artificial selection for alcohol sensitivity for 35 generation. In each generations we scored 60 males and females,…
uses data from: http://identifiers.org/pubmed/17973985

Sr-CIV UP in 'ethanol resistant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.2720647625
p value: 0.00337864182520998
refers to: 1624817_at
refers to: Sr-CIV
t statistic: 7.23863512923563

(Assay) resistant2f1
type: Assay
has participant: A-AFFY-35

(Assay) resistant1m2
type: Assay
has participant: A-AFFY-35

CG9837 DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.128272025
p value: 0.00337864182520998
refers to: 1639101_at
refers to: CG9837
t statistic: -7.33809650315898

LysP DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.232750125
p value: 0.0189789273632806
refers to: LysP
refers to: 1636767_at
t statistic: -5.742985694372

(Assay) sensitive2m1
type: Assay
has participant: A-AFFY-35

CG11034
type: EnsemblDatabaseReference

(Sample) organism/Drosophila melanogaster
type: Drosophila melanogaster
property type: organism
property value: Drosophila melanogaster

CG30083
type: EnsemblDatabaseReference

Sr-CIV
type: EnsemblDatabaseReference

Cyp6a23
type: EnsemblDatabaseReference

CG9837
type: EnsemblDatabaseReference

CG10559
type: EnsemblDatabaseReference

CG15661
type: EnsemblDatabaseReference

1635033_at
type: EFO_0005072
part of: A-AFFY-35

(Assay) control2f1
type: Assay
has participant: A-AFFY-35

(Assay) sensitive1m1
type: Assay
has participant: A-AFFY-35

1636409_at
type: EFO_0005072
part of: A-AFFY-35

Jon66Ci
type: EnsemblDatabaseReference

Cyp6a23
type: EnsemblDatabaseReference

(Factor value) PHENOTYPE/ethanol sensitive
type: EFO_0000001
property type: PHENOTYPE
property value: ethanol sensitive

CG33337 UP in 'ethanol resistant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.5058616375
p value: 6.09158205633724E-4
refers to: 1635441_at
refers to: CG33337
t statistic: 8.62394521819978

CG33337
type: EnsemblDatabaseReference

Cyp28a5
type: EnsemblDatabaseReference

CG18417
type: EnsemblDatabaseReference

(Assay) resistant2m1
type: Assay
has participant: A-AFFY-35

CG13313 UP in 'ethanol sensitive' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.4013946375
p value: 0.0312382028901205
refers to: CG13313
refers to: 1641268_at
t statistic: 6.21685929638824

LysP
type: EnsemblDatabaseReference

CG17752 DOWN in 'ethanol sensitive' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.027088275
p value: 0.0364215303387745
refers to: CG17752
refers to: 1627080_at
t statistic: -5.80228398610504

CG33109
type: EnsemblDatabaseReference

(Assay) control2f2
type: Assay
has participant: A-AFFY-35

(Assay) control2m1
type: Assay
has participant: A-AFFY-35

(Assay) sensitive1f1
type: Assay
has participant: A-AFFY-35

CG32751
type: EnsemblDatabaseReference

CG17752
type: EnsemblDatabaseReference

(Assay) sensitive2m2
type: Assay
has participant: A-AFFY-35

LysP
type: EnsemblDatabaseReference

(Sample) age/3 to 5
type: EFO_0000001
property type: age
property value: 3 to 5

1625873_at
type: EFO_0005072
part of: A-AFFY-35

(Assay) control2m2
type: Assay
has participant: A-AFFY-35

A-AFFY-35
type: EFO_0000269

(Assay) resistant1f1
type: Assay
has participant: A-AFFY-35

CG32751 DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.60972675
p value: 0.0189789273632806
refers to: CG32751
refers to: 1630310_at
t statistic: -5.78249472526036

CG30083
type: EnsemblDatabaseReference

CG5724
type: EnsemblDatabaseReference

CG9837
type: EnsemblDatabaseReference

Cyp6a17
type: EnsemblDatabaseReference

1636767_at
type: EFO_0005072
part of: A-AFFY-35

Cyp28a5 DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.1304485125
p value: 0.00337864182520998
refers to: Cyp28a5
refers to: 1629009_at
t statistic: -7.24333442925663

CG13313
type: EnsemblDatabaseReference

CG15661 UP in 'ethanol sensitive' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.2484085625
p value: 0.0317873655078275
refers to: 1625873_at
refers to: CG15661
t statistic: 6.12208990013371

CG11034 DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.08600465
p value: 0.0308732609491393
refers to: 1636409_at
refers to: CG11034
t statistic: -5.29102691204864

PGRP-SC1b DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.61981225
p value: 0.0167096301406057
refers to: PGRP-SC1b
refers to: 1627986_s_at
t statistic: -5.93182991792037

(Assay) control1f1
type: Assay
has participant: A-AFFY-35

(Assay) resistant1m1
type: Assay
has participant: A-AFFY-35

1635441_at
type: EFO_0005072
part of: A-AFFY-35

(Sample) sex/female
type: EFO_0001265
property type: sex
property value: female

CG5724 DOWN in 'ethanol sensitive' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.2353112875
p value: 0.0470773811635177
refers to: CG5724
refers to: 1638903_at
t statistic: -5.43523388227208

Cyp6a17
type: EnsemblDatabaseReference

CG33337
type: EnsemblDatabaseReference

Analysis of 'ethanol sensitive' vs 'wild type'
type: MicroarrayDifferentialExpressionAnalysis

CG18417 UP in 'ethanol resistant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.403743175
p value: 0.0461841465108341
refers to: 1630590_at
refers to: CG18417
t statistic: 5.00265794002106

CG6012
type: EnsemblDatabaseReference

Cyp28a5
type: EnsemblDatabaseReference

1641268_at
type: EFO_0005072
part of: A-AFFY-35

1627986_s_at
type: EFO_0005072
part of: A-AFFY-35

CG6385
type: EnsemblDatabaseReference

(Sample) phenotype/wild type genotype
type: EFO_0005168
property type: phenotype
property value: wild type genotype

(Assay) resistant1f2
type: Assay
has participant: A-AFFY-35

1639819_at
type: EFO_0005072
part of: A-AFFY-35

(Assay) resistant2m2
type: Assay
has participant: A-AFFY-35

(Assay) sensitive2f1
type: Assay
has participant: A-AFFY-35

1624817_at
type: EFO_0005072
part of: A-AFFY-35

Cyp6a17 UP in 'ethanol resistant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.0689448125
p value: 0.0354941496062724
refers to: Cyp6a17
refers to: 1628052_at
t statistic: 5.18446673271589

CG33109
type: EnsemblDatabaseReference

1625538_at
type: EFO_0005072
part of: A-AFFY-35

1627817_at
type: EFO_0005072
part of: A-AFFY-35

CG6012
type: EnsemblDatabaseReference

1629009_at
type: EFO_0005072
part of: A-AFFY-35

CG30083 DOWN in 'ethanol sensitive' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.1978061375
p value: 0.0135944327797303
refers to: CG30083
refers to: 1635033_at
t statistic: -7.09136654346204

CG32751
type: EnsemblDatabaseReference

CG17752
type: EnsemblDatabaseReference

(Assay) sensitive1f2
type: Assay
has participant: A-AFFY-35

(Factor value) PHENOTYPE/wild type genotype
type: EFO_0005168
property type: PHENOTYPE
property value: wild type genotype

(Sample) sex/male
type: EFO_0001266
property type: sex
property value: male

Drosophila melanogaster
type: OBI_0100026

1630310_at
type: EFO_0005072
part of: A-AFFY-35

1639101_at
type: EFO_0005072
part of: A-AFFY-35

(Assay) control1m2
type: Assay
has participant: A-AFFY-35

CG6012 DOWN in 'ethanol sensitive' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.2825700875
p value: 0.0366481002423257
refers to: CG6012
refers to: 1632808_at
t statistic: -5.75227683752879

PGRP-SC1b
type: EnsemblDatabaseReference

1624101_at
type: EFO_0005072
part of: A-AFFY-35

CG10559 DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.1716630375
p value: 0.00402679435391376
refers to: CG10559
refers to: 1638388_at
t statistic: -7.06370888114204

CG10559
type: EnsemblDatabaseReference

1638903_at
type: EFO_0005072
part of: A-AFFY-35

(Assay) sensitive2f2
type: Assay
has participant: A-AFFY-35

CG15661
type: EnsemblDatabaseReference

Jon66Ci
type: EnsemblDatabaseReference

CG13313
type: EnsemblDatabaseReference

CG5724
type: EnsemblDatabaseReference

(Assay) resistant2f2
type: Assay
has participant: A-AFFY-35

1627080_at
type: EFO_0005072
part of: A-AFFY-35

CG33109 DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -2.7907630125
p value: 0.01661986487873
refers to: CG33109
refers to: 1627817_at
t statistic: -6.00957015950165

PGRP-SC1b
type: EnsemblDatabaseReference

(Sample) phenotype/ethanol sensitive
type: EFO_0000001
property type: phenotype
property value: ethanol sensitive

(Sample) phenotype/ethanol resistant
type: EFO_0000001
property type: phenotype
property value: ethanol resistant

1638388_at
type: EFO_0005072
part of: A-AFFY-35

CG18417
type: EnsemblDatabaseReference

(Assay) control1f2
type: Assay
has participant: A-AFFY-35

(Assay) sensitive1m2
type: Assay
has participant: A-AFFY-35

CG6385 DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.04685765
p value: 1.76670158018794E-5
refers to: 1639819_at
refers to: CG6385
t statistic: -12.6246111778252

Cyp6a23 DOWN in 'ethanol resistant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -2.3692621625
p value: 3.21434778554669E-4
refers to: 1624101_at
refers to: Cyp6a23
t statistic: -9.71253757530152

Analysis of 'ethanol resistant' vs 'wild type'
type: MicroarrayDifferentialExpressionAnalysis

(Factor value) PHENOTYPE/ethanol resistant
type: EFO_0000001
property type: PHENOTYPE
property value: ethanol resistant

CG6385
type: EnsemblDatabaseReference

Jon66Ci DOWN in 'ethanol sensitive' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -2.353922175
p value: 0.0135944327797303
refers to: 1625538_at
refers to: Jon66Ci
t statistic: -7.40983969259268

CG11034 DOWN in 'ethanol sensitive' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -2.129010025
p value: 0.0135944327797303
refers to: CG11034
refers to: 1636409_at
t statistic: -7.22460536573766

1632808_at
type: EFO_0005072
part of: A-AFFY-35