Eif2s3y UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Grap2 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Cmpk2 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Kdm5d UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Mapk14 DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
(Assay) p38alpha knock out fetal liver E15.5, biological rep3
p38alpha knock out fetal liver E15.5, biological rep3
Isg15 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
(Assay) p38alpha wild type fetal liver E13.5, biological rep2
p38alpha wild type fetal liver E13.5, biological rep2
(Assay) p38alpha wild type fetal liver E15.5, biological rep1
p38alpha wild type fetal liver E15.5, biological rep1
Gadd45g UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
(Assay) p38alpha knock out fetal liver E15.5, biological rep2
p38alpha knock out fetal liver E15.5, biological rep2
(Assay) p38alpha wild type fetal liver E13.5, biological rep1
p38alpha wild type fetal liver E13.5, biological rep1
Mapk14 DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
(Sample) developmental stage/embryonic day 15.5
Dusp8 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Pm20d1 DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
(Sample) genetic modification/gene knock out
Analysis of 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
(Assay) p38alpha knock out fetal liver E13.5, biological rep3
p38alpha knock out fetal liver E13.5, biological rep3
Chil3 DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Hbb-bh1 DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
(Assay) p38alpha wild type fetal liver E15.5, biological rep3
p38alpha wild type fetal liver E15.5, biological rep3
Dusp8 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Jun UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Ddx3y UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
(Sample) developmental stage/embryonic day 13.5
Analysis of 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
(Assay) p38alpha knock out fetal liver E13.5, biological rep1
p38alpha knock out fetal liver E13.5, biological rep1
(Assay) p38alpha wild type fetal liver E13.5, biological rep3
p38alpha wild type fetal liver E13.5, biological rep3
Cd5l DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
(Assay) p38alpha knock out fetal liver E15.5, biological rep1
p38alpha knock out fetal liver E15.5, biological rep1
Agxt DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Xist DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Mmel1 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
(Factor value) DEVELOPMENTAL_STAGE/embryonic day 13.5
Jun UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Glrx UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Itgb7 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Uty UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
(Factor value) DEVELOPMENTAL_STAGE/embryonic day 15.5
(Factor value) GENOTYPE/wild type genotype
Gadd45g UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Fhl1 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Ebf2 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
The mitogen-activated protein kinase (MAPK) p38alpha controls inflammatory responses and cell proliferation. Using mice carrying conditional p38alpha alleles, we investigated its function in postnatal development and tumorigenesis. When p38alpha is specifically deleted in the mouse embryo, fetuses develop to term but die shortly after birth, likely due to lung dysfunction. Fetal hematopoietic cells and embryonic fibroblasts deficient in p38alpha display increased proliferation, resulting from sustained activation of the c-Jun N-terminal kinase (JNK)/c-Jun pathway. Importantly, in chemical-induced liver cancer development, mice with liver-specific deletion of p38alpha show enhanced hepatocyte proliferation and tumor development that also correlates with JNK/c-Jun upregulation. Furthermore, increased proliferation of p38alpha-deficient hepatocytes and tumor cells is suppressed by inactivation of JNK or c-Jun. These results reveal a novel mechanism whereby p38alpha negatively regulates cell proliferation through antagonizing the JNK/c-Jun pathway in multiple cell types and in liver cancer development. We used microarrays to identifiy differental regulated genes by p38alpha in fetal liver cells Experiment Overall Design: Wild type and p38 deficient fetal liver cell were used for RNA extraction and hybridization on Affymetrix microarrays.
(Assay) p38alpha wild type fetal liver E15.5, biological rep2
p38alpha wild type fetal liver E15.5, biological rep2
Ctse DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Tnnc2 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
Kdm6a DOWN in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 15.5'
Grap2 UP in 'p38alpha null' vs 'wild type genotype' in 'embryonic day 13.5'
(Assay) p38alpha knock out fetal liver E13.5, biological rep2
p38alpha knock out fetal liver E13.5, biological rep2