HES1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -2.02668618307512
p value: 6.01042483212652E-4
refers to: HES1
refers to: 203394_s_at
t statistic: -12.6776623834446

S1PR3 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.18587982963525
p value: 0.00545074670476492
refers to: S1PR3
refers to: 228176_at
t statistic: 7.25312868729195

223168_at
type: EFO_0005072
part of: A-AFFY-44

SH3TC1
type: EnsemblDatabaseReference

225308_s_at
type: EFO_0005072
part of: A-AFFY-44

DAAM2
type: EnsemblDatabaseReference

MSH6
type: EnsemblDatabaseReference

FKBP11
type: EnsemblDatabaseReference

ID1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.23147837538635
p value: 9.08139031544392E-4
refers to: ID1
refers to: 208937_s_at
t statistic: -11.6471490258695

DTX1
type: EnsemblDatabaseReference

(Factor value) COMPOUND/none
type: EFO_0001461
property type: COMPOUND
property value: none

206591_at
type: EFO_0005072
part of: A-AFFY-44

S1PR3
type: EnsemblDatabaseReference

203504_s_at
type: EFO_0005072
part of: A-AFFY-44

MIER1
type: EnsemblDatabaseReference

E2F7
type: EnsemblDatabaseReference

HSPA5 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.03625836897186
p value: 0.00690326724018106
refers to: HSPA5
refers to: 211936_at
t statistic: -6.46386903267093

244033_at
type: EFO_0005072
part of: A-AFFY-44

219389_at
type: EFO_0005072
part of: A-AFFY-44

FAM46C DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.77830403439788
p value: 1.56427914103137E-4
refers to: FAM46C
refers to: 226811_at
t statistic: -15.6297520819157

(Assay) MOLT4_ctrl_A
type: Assay
has participant: A-AFFY-44

HES1
type: EnsemblDatabaseReference

CCR9
type: EnsemblDatabaseReference

BAHCC1
type: EnsemblDatabaseReference

223821_s_at
type: EFO_0005072
part of: A-AFFY-44

NOTCH3
type: EnsemblDatabaseReference

LZTFL1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.4273350559792
p value: 6.67690704954528E-4
refers to: LZTFL1
refers to: 218437_s_at
t statistic: -12.2869479037632

SATB1
type: EnsemblDatabaseReference

ABCA1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.41107714205299
p value: 0.0112809566688103
refers to: ABCA1
refers to: 203505_at
t statistic: -5.31816807323029

SLC1A4 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.11153922092749
p value: 0.0254323185129324
refers to: SLC1A4
refers to: 244377_at
t statistic: -3.94932462273015

HES4
type: EnsemblDatabaseReference

EEA1 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.02938769415157
p value: 0.00678018523378015
refers to: 225885_at
refers to: EEA1
t statistic: 6.5313768558118

RCBTB2
type: EnsemblDatabaseReference

(Sample) cell line/MOLT-4
type: EFO_0001220
property type: cell line
property value: MOLT-4

227336_at
type: EFO_0005072
part of: A-AFFY-44

(Assay) MOLT4_GSI_B
type: Assay
has participant: A-AFFY-44

ID1
type: EnsemblDatabaseReference

LZTFL1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.51424113055661
p value: 4.94916940072806E-4
refers to: LZTFL1
refers to: 222632_s_at
t statistic: -13.2652067132582

SUSD4 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.05933466941485
p value: 0.00525833604768567
refers to: 219389_at
refers to: SUSD4
t statistic: -7.41077694033127

209757_s_at
type: EFO_0005072
part of: A-AFFY-44

MSH6
type: EnsemblDatabaseReference

(Assay) MOLT4_ctrl_C
type: Assay
has participant: A-AFFY-44

207445_s_at
type: EFO_0005072
part of: A-AFFY-44

219218_at
type: EFO_0005072
part of: A-AFFY-44

ABCA1
type: EnsemblDatabaseReference

RCBTB2 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -2.24065002725812
p value: 1.41852159461171E-4
refers to: RCBTB2
refers to: 204759_at
t statistic: -16.0642500815202

LZTFL1
type: EnsemblDatabaseReference

SUSD4
type: EnsemblDatabaseReference

LINC01215
type: EnsemblDatabaseReference

RHOU DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -2.86523725173138
p value: 2.90178244095965E-5
refers to: RHOU
refers to: 223168_at
t statistic: -21.3881494346523

SUSD4 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.11764307016126
p value: 0.00311282438101712
refers to: SUSD4
refers to: 223821_s_at
t statistic: -8.92980307807737

(Assay) MOLT4_ctrl_B
type: Assay
has participant: A-AFFY-44

TXNIP UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.54352687485495
p value: 0.0249097563785923
refers to: 201010_s_at
refers to: TXNIP
t statistic: 3.98356667849746

E2F7 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.1087194256983
p value: 0.00130725632482005
refers to: 228033_at
refers to: E2F7
t statistic: 10.8411897275542

CCR9 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.18776207657535
p value: 0.00525442669783192
refers to: CCR9
refers to: 207445_s_at
t statistic: -7.43951163507314

FKBP11
type: EnsemblDatabaseReference

TOB1
type: EnsemblDatabaseReference

HES4
type: EnsemblDatabaseReference

E-GEOD-6495
type: microarray 1colour mrna differential
RO_0002162: Homo sapiens
description: In T-cell acute lymphoblastic leukemia (T-ALL) NOTCH 1 receptors are frequently mutated. This leads to aberrantly high Notch signaling, but how this translates into deregulated cell cycle control and the transformed cell type is poorly understood. In this report, we analyze downstream responses resulting from the high level of NOTCH 1 signaling in T-ALL. Notch activity, measured immediately downstream of the NOTCH 1 receptor, is high, but expression of the canonical downstream Notch response genes HES 1 and HEY 2 is low both in primary cells from T-ALL patients and in T-ALL cell lines. This suggests that other immediate Notch downstream genes are activated, and we found that Notch signaling controls the levels of expression of the E3 ubiquitin ligase SKP2 and its target protein p27Kip1. We show that in T-ALL cell lines, recruitment of NOTCH 1 ICD to the SKP2 promoter was accompanied by high SKP2 and low p27Kip1 protein levels were low. In contrast, pharmacologically blocking Notch signaling reversed this picture and led to loss of NOTCH 1 ICD occupancy of the SKP2 promoter, decreased SKP2 and increased p27Kip1 expression. T-ALL cells show a rapid G1-S cell cycle transition, while blocked Notch signaling resulted in G0/G1 cell cycle arrest, also observed by transfection of p27Kip1 or, to a smaller extent, a dominant negative SKP2 allele. Collectively, our data suggest that the aberrantly high Notch signaling in T-ALL maintains SKP2 at a high level and reduces p27Kip1, which leads to more rapid cell cycle progression. Experiment Overall Design: Three independent cultures of the MOLT4 cell line before and 48 hours after addition of the gamma-secretase inhibitor DAPT (5 uM).
uses data from: http://identifiers.org/pubmed/17560996

244377_at
type: EFO_0005072
part of: A-AFFY-44

ELOVL4 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.38533872166755
p value: 0.00398820570444108
refers to: ELOVL4
refers to: 219532_at
t statistic: 8.28514669220006

A-AFFY-44
type: EFO_0000269

DTX1
type: EnsemblDatabaseReference

TANC1
type: EnsemblDatabaseReference

225885_at
type: EFO_0005072
part of: A-AFFY-44

NOTCH3 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.85280671816825
p value: 3.19210083349849E-4
refers to: NOTCH3
refers to: 203238_s_at
t statistic: -14.1578615191385

226811_at
type: EFO_0005072
part of: A-AFFY-44

S1PR3
type: EnsemblDatabaseReference

E2F7
type: EnsemblDatabaseReference

228033_at
type: EFO_0005072
part of: A-AFFY-44

MYCN
type: EnsemblDatabaseReference

224516_s_at
type: EFO_0005072
part of: A-AFFY-44

RASGRP1
type: EnsemblDatabaseReference

211450_s_at
type: EFO_0005072
part of: A-AFFY-44

201010_s_at
type: EFO_0005072
part of: A-AFFY-44

RGCC UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.4698285991123
p value: 0.00641572754780245
refers to: RGCC
refers to: 218723_s_at
t statistic: 6.69383029093246

FKBP11 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.20332333382267
p value: 0.00642068534229706
refers to: FKBP11
refers to: 219118_at
t statistic: -6.68265315174586

218723_s_at
type: EFO_0005072
part of: A-AFFY-44

Homo sapiens
type: OBI_0100026

236198_at
type: EFO_0005072
part of: A-AFFY-44

211936_at
type: EFO_0005072
part of: A-AFFY-44

203505_at
type: EFO_0005072
part of: A-AFFY-44

227347_x_at
type: EFO_0005072
part of: A-AFFY-44

(Assay) MOLT4_GSI_C
type: Assay
has participant: A-AFFY-44

MYO7B DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.26748183256158
p value: 0.00130725632482005
refers to: MYO7B
refers to: 235383_at
t statistic: -10.8736518188196

RCBTB2
type: EnsemblDatabaseReference

DTX1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -2.21192578434978
p value: 2.90178244095965E-5
refers to: 227336_at
refers to: DTX1
t statistic: -22.1159379585203

208937_s_at
type: EFO_0005072
part of: A-AFFY-44

CR2
type: EnsemblDatabaseReference

(Assay) MOLT4_GSI_A
type: Assay
has participant: A-AFFY-44

CEP128
type: EnsemblDatabaseReference

SUSD4
type: EnsemblDatabaseReference

(Sample) cell type/T-cell acute lymphoblastic leukemia cell
type: EFO_0000209
property type: cell type
property value: T-cell acute lymphoblastic leukemia cell

TOB1 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.01592533915549
p value: 0.0106324832529223
refers to: TOB1
refers to: 202704_at
t statistic: 5.54818677220694

Analysis of '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: MicroarrayDifferentialExpressionAnalysis

228176_at
type: EFO_0005072
part of: A-AFFY-44

CD55
type: EnsemblDatabaseReference

203394_s_at
type: EFO_0005072
part of: A-AFFY-44

HES4 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -3.92547230220008
p value: 5.11357347397821E-5
refers to: HES4
refers to: 227347_x_at
t statistic: -19.3519021209513

P2RX5 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.3816458054313
p value: 0.00220217054652023
refers to: 210448_s_at
refers to: P2RX5
t statistic: -9.62155037988879

CD82
type: EnsemblDatabaseReference

HSPA5
type: EnsemblDatabaseReference

HES1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.05887448426362
p value: 0.00479611657367341
refers to: HES1
refers to: 203395_s_at
t statistic: -7.79715830693947

EEA1
type: EnsemblDatabaseReference

ELOVL4
type: EnsemblDatabaseReference

TOB1
type: EnsemblDatabaseReference

203395_s_at
type: EFO_0005072
part of: A-AFFY-44

TANC1
type: EnsemblDatabaseReference

202704_at
type: EFO_0005072
part of: A-AFFY-44

219256_s_at
type: EFO_0005072
part of: A-AFFY-44

LZTFL1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.65744845627806
p value: 0.0120756123921328
refers to: LZTFL1
refers to: 232418_at
t statistic: -5.18046224518876

SH2D1A
type: EnsemblDatabaseReference

MIER1 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.03478194189322
p value: 0.00193962315424274
refers to: 225475_at
refers to: MIER1
t statistic: 9.99804980012754

204759_at
type: EFO_0005072
part of: A-AFFY-44

RAG1
type: EnsemblDatabaseReference

1555950_a_at
type: EFO_0005072
part of: A-AFFY-44

MYO7B
type: EnsemblDatabaseReference

ABCA1
type: EnsemblDatabaseReference

RASGRP1 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.40924174597566
p value: 5.19088750851026E-4
refers to: 205590_at
refers to: RASGRP1
t statistic: 13.0392533668039

MYCN DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.3899738419486
p value: 1.07001235188943E-4
refers to: MYCN
refers to: 209757_s_at
t statistic: -16.9144209760366

DAAM2 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.18654368748752
p value: 7.43932846510809E-4
refers to: DAAM2
refers to: 212793_at
t statistic: 12.0263547373054

RGCC
type: EnsemblDatabaseReference

LINC01215
type: EnsemblDatabaseReference

RASGRP1
type: EnsemblDatabaseReference

219497_s_at
type: EFO_0005072
part of: A-AFFY-44

205590_at
type: EFO_0005072
part of: A-AFFY-44

CEP128 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.37289310369647
p value: 9.91823428350608E-4
refers to: 244033_at
refers to: CEP128
t statistic: 11.3587776466636

225475_at
type: EFO_0005072
part of: A-AFFY-44

BAHCC1 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.19552858942009
p value: 0.00545074670476492
refers to: BAHCC1
refers to: 219218_at
t statistic: 7.26754380058664

BCL11A
type: EnsemblDatabaseReference

TXNIP
type: EnsemblDatabaseReference

LINC01215 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.0734513677624
p value: 0.00418883735618412
refers to: LINC01215
refers to: 236198_at
t statistic: -8.09627271404209

(Factor value) COMPOUND/gamma-secretase inhibitor DAPT 5
type: EFO_0000001
property type: COMPOUND
property value: gamma-secretase inhibitor DAPT 5

ABCA1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.40005003561072
p value: 0.0017013271039354
refers to: 203504_s_at
refers to: ABCA1
t statistic: -10.3166644568087

RHOU
type: EnsemblDatabaseReference

228910_at
type: EFO_0005072
part of: A-AFFY-44

BCL11A UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.00772938004135
p value: 0.00311413074849441
refers to: BCL11A
refers to: 219497_s_at
t statistic: 8.8481389035978

241365_at
type: EFO_0005072
part of: A-AFFY-44

SLC1A4
type: EnsemblDatabaseReference

MSH6 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.04133704852081
p value: 0.00220217054652023
refers to: 211450_s_at
refers to: MSH6
t statistic: 9.68020686606817

CR2
type: EnsemblDatabaseReference

212793_at
type: EFO_0005072
part of: A-AFFY-44

FAM46C
type: EnsemblDatabaseReference

CEP128
type: EnsemblDatabaseReference

P2RX5
type: EnsemblDatabaseReference

CR2 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -3.05428930576232
p value: 0.0015931090944787
refers to: CR2
refers to: 205544_s_at
t statistic: -10.4577371403676

CD82 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.29230897449925
p value: 0.00454873211289407
refers to: CD82
refers to: 228910_at
t statistic: -7.90671179164634

219532_at
type: EFO_0005072
part of: A-AFFY-44

205544_s_at
type: EFO_0005072
part of: A-AFFY-44

CD55
type: EnsemblDatabaseReference

MYCN
type: EnsemblDatabaseReference

CXXC5
type: EnsemblDatabaseReference

EEA1
type: EnsemblDatabaseReference

ELOVL4
type: EnsemblDatabaseReference

232418_at
type: EFO_0005072
part of: A-AFFY-44

SH3TC1
type: EnsemblDatabaseReference

(Sample) organism/Homo sapiens
type: Homo sapiens
property type: organism
property value: Homo sapiens

DAAM2
type: EnsemblDatabaseReference

219118_at
type: EFO_0005072
part of: A-AFFY-44

218437_s_at
type: EFO_0005072
part of: A-AFFY-44

RAG1 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 2.33095247750455
p value: 2.90178244095965E-5
refers to: RAG1
refers to: 206591_at
t statistic: 21.7757009581858

235383_at
type: EFO_0005072
part of: A-AFFY-44

SH2D1A
type: EnsemblDatabaseReference

210116_at
type: EFO_0005072
part of: A-AFFY-44

MYO7B
type: EnsemblDatabaseReference

MIER1
type: EnsemblDatabaseReference

222632_s_at
type: EFO_0005072
part of: A-AFFY-44

RGCC
type: EnsemblDatabaseReference

SATB1 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.07952259952069
p value: 0.00398820570444108
refers to: SATB1
refers to: 241365_at
t statistic: 8.32920869526926

(Sample) genotype/NOTCH 1 mutation
type: EFO_0000001
property type: genotype
property value: NOTCH 1 mutation

HES1
type: EnsemblDatabaseReference

203238_s_at
type: EFO_0005072
part of: A-AFFY-44

BCL11A
type: EnsemblDatabaseReference

210448_s_at
type: EFO_0005072
part of: A-AFFY-44

TXNIP
type: EnsemblDatabaseReference

TANC1 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.00523928112152
p value: 0.00902598914896893
refers to: 225308_s_at
refers to: TANC1
t statistic: 5.89388266933968

HSPA5
type: EnsemblDatabaseReference

CD82
type: EnsemblDatabaseReference

CCR9
type: EnsemblDatabaseReference

BAHCC1
type: EnsemblDatabaseReference

CXXC5 UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.07730303201872
p value: 0.00190677484798072
refers to: CXXC5
refers to: 224516_s_at
t statistic: 10.068020674397

RHOU
type: EnsemblDatabaseReference

NOTCH3
type: EnsemblDatabaseReference

SH2D1A UP in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: IncreasedDifferentialExpressionRatio
fold change: 1.09576394840628
p value: 9.91823428350608E-4
refers to: SH2D1A
refers to: 210116_at
t statistic: 11.4272777671842

SATB1
type: EnsemblDatabaseReference

SLC1A4
type: EnsemblDatabaseReference

SH3TC1 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.31429714079006
p value: 6.27074799270987E-4
refers to: SH3TC1
refers to: 219256_s_at
t statistic: -12.4917931806475

FAM46C
type: EnsemblDatabaseReference

P2RX5
type: EnsemblDatabaseReference

RAG1
type: EnsemblDatabaseReference

LZTFL1
type: EnsemblDatabaseReference

CD55 DOWN in '5 micromolar gamma-secretase inhibitor DAPT' vs 'none'
type: DecreasedDifferentialExpressionRatio
fold change: -1.02189664803387
p value: 0.00525442669783192
refers to: CD55
refers to: 1555950_a_at
t statistic: -7.43279548024469

ID1
type: EnsemblDatabaseReference

CXXC5
type: EnsemblDatabaseReference