Gys1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Acly DOWN in '25 month, normal diet' vs '5 month, normal diet'
Col1a2 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Gm21967 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Speg DOWN in '25 month, normal diet' vs '5 month, normal diet'
Actc1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Lgals3 UP in '25 month, normal diet' vs '5 month, normal diet'
Snrpn DOWN in '25 month, normal diet' vs '5 month, normal diet'
Egln3 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Tspan7 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Ighm UP in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep2
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep2
(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep2
Gastrocnemius tissue at time 0 from young mouse, biological rep2
Ankrd2 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Col1a1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep5
Gastrocnemius tissue at time 0 from young mouse, biological rep5
(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep3
Gastrocnemius tissue at time 0 from aged mouse, biological rep3
Acaa2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Tmem109 DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Sample) organism part/gastrocnemius muscle
Akt1 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Gpd1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Fasn DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep6
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep6
Igj UP in '25 month, normal diet' vs '5 month, normal diet'
Scd1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Col1a1 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Analysis of '25 month, normal diet' vs '5 month, normal diet'
Tpm3 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Anxa6 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Igkc UP in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep7
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep7
Col1a2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Ywhag DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep4
Gastrocnemius tissue at time 0 from aged mouse, biological rep4
(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep1
Gastrocnemius tissue at time 0 from aged mouse, biological rep1
Amy1 UP in '25 month, normal diet' vs '5 month, normal diet'
Arf6 UP in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep2
Gastrocnemius tissue at time 0 from aged mouse, biological rep2
Pfkfb3 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Capzb DOWN in '25 month, normal diet' vs '5 month, normal diet'
Aplp2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Rnf14 DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Factor value) DIET/calorie-restricted diet
Pdha1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep3
Gastrocnemius tissue at time 0 from young mouse, biological rep3
(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep4
Gastrocnemius tissue at time 0 from young mouse, biological rep4
Spop DOWN in '25 month, normal diet' vs '5 month, normal diet'
Camk2a DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep1
Gastrocnemius tissue at time 0 from young mouse, biological rep1
Chac1 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Sln UP in '25 month, normal diet' vs '5 month, normal diet'
Amy1 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Analysis of '25 month, calorie-restricted diet' vs '5 month, normal diet'
Col3a1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Gbp6 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Col3a1 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
Ncl DOWN in '25 month, normal diet' vs '5 month, normal diet'
Zfp106 DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep5
Gastrocnemius tissue at time 0 from aged mouse, biological rep5
Dusp10 DOWN in '25 month, normal diet' vs '5 month, normal diet'
(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep3
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep3
Atp1a2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
Aplnr DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
We investigated age-related changes in the transcriptional profile of skeletal muscle in 5 month old (young) and 25 month old (old) C57BL/6NHsd mice using high density oligonucleotide arrays (22,690 transcripts probed). We identified 712 transcripts that are differentially expressed in young (5 month old) and old (25-month old) mouse skeletal muscle. Caloric restriction (CR) completely or partially reversed 87% of the changes in expression. Examination of individual genes revealed a transcriptional profile indicative of increased p53 activity in the older muscle. To determine whether the increase in p53 activity is associated with transcriptional activation of apoptotic targets, we performed RT-PCR on four well known mediators of p53-induced apoptosis: puma, noxa, tnfrsf10b and bok. Expression levels for these proapoptotic genes increased significantly with age (P<0.05), while CR significantly lowered expression levels for these genes as compared to control fed old mice (P<0.05). Age-related induction of p53-related genes was observed in multiple tissues, but was not observed in SOD2+/- and GPX4+/- mice, suggesting that oxidative stress does not mediate the observed age-related increase in expression. Western blot analysis confirmed that protein levels for both p21 and GADD45a, two established transcriptional targets of p53, were higher in the older muscle tissue. These observations support a role for p53-mediated apoptotic activity in mammalian aging. Experiment Overall Design: Animals were killed by cervical dislocation. Five different animals were used for each experimental group. All collected gastrocnemius tissue was then dissected, placed in a microcentrifuge tube, flash-frozen in liquid nitrogen, and stored at -80ÃÂ°C until processed for microarrays.
(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep5
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep5