1431030_a_at
type: EFO_0005072
part of: A-AFFY-36

Gys1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.10185545388627
p value: 0.0253324109511537
refers to: Gys1
refers to: 1450196_s_at
t statistic: -3.60929528441691

Acly DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.23268220142674
p value: 0.0191621670395962
refers to: 1425326_at
refers to: Acly
t statistic: -4.02273732682565

Col1a2 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.3282977757581
p value: 0.00553575988199005
refers to: Col1a2
refers to: 1450857_a_at
t statistic: -7.31260827049045

Scd1
type: EnsemblDatabaseReference

1418649_at
type: EFO_0005072
part of: A-AFFY-36

Gm21967 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.15859488586527
p value: 0.0106395439835764
refers to: Gm21967
refers to: 1426607_at
t statistic: -5.14348214138612

Igkc
type: EnsemblDatabaseReference

Gpd1
type: EnsemblDatabaseReference

1415965_at
type: EFO_0005072
part of: A-AFFY-36

Col1a1
type: EnsemblDatabaseReference

Camk2a
type: EnsemblDatabaseReference

Speg DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.02977940424278
p value: 0.0207686588728281
refers to: Speg
refers to: 1451886_at
t statistic: -3.88384156240337

Actc1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.80871984505035
p value: 0.00875188429082373
refers to: 1415927_at
refers to: Actc1
t statistic: -5.44858405684341

1421063_s_at
type: EFO_0005072
part of: A-AFFY-36

Lgals3 UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.21440261648278
p value: 0.011944621811497
refers to: Lgals3
refers to: 1426808_at
t statistic: 4.86382097076184

Ighm
type: EnsemblDatabaseReference

Snrpn DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.37674707625151
p value: 0.0142112794643368
refers to: Snrpn
refers to: 1421063_s_at
t statistic: -4.59775979826394

1438651_a_at
type: EFO_0005072
part of: A-AFFY-36

1426808_at
type: EFO_0005072
part of: A-AFFY-36

Rnf14
type: EnsemblDatabaseReference

Tspan7
type: EnsemblDatabaseReference

1427351_s_at
type: EFO_0005072
part of: A-AFFY-36

Actc1
type: EnsemblDatabaseReference

Anxa6
type: EnsemblDatabaseReference

Egln3 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.5984568887523
p value: 0.00461876576564258
refers to: Egln3
refers to: 1418649_at
t statistic: 8.15652229191023

Tspan7 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.31265072293933
p value: 0.0145868576542379
refers to: 1417502_at
refers to: Tspan7
t statistic: -4.55713053877287

Tspan7
type: EnsemblDatabaseReference

Gbp6
type: EnsemblDatabaseReference

Ighm UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.59841563472257
p value: 0.00963703554235455
refers to: Ighm
refers to: 1427351_s_at
t statistic: 5.32390241820982

1427883_a_at
type: EFO_0005072
part of: A-AFFY-36

1416204_at
type: EFO_0005072
part of: A-AFFY-36

1438676_at
type: EFO_0005072
part of: A-AFFY-36

Acly DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.01254952359192
p value: 0.0112446111772239
refers to: Acly
refers to: 1451666_at
t statistic: -5.05132048537315

Col3a1
type: EnsemblDatabaseReference

1449137_at
type: EFO_0005072
part of: A-AFFY-36

Pfkfb3
type: EnsemblDatabaseReference

Amy1
type: EnsemblDatabaseReference

1423828_at
type: EFO_0005072
part of: A-AFFY-36

(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep2
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep2
has participant: A-AFFY-36

(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep2
type: Assay
identifier: Gastrocnemius tissue at time 0 from young mouse, biological rep2
has participant: A-AFFY-36

Egln3
type: EnsemblDatabaseReference

Ncl
type: EnsemblDatabaseReference

(Sample) age/5
type: EFO_0000001
property type: age
property value: 5

Tmem109
type: EnsemblDatabaseReference

Ywhag
type: EnsemblDatabaseReference

1449996_a_at
type: EFO_0005072
part of: A-AFFY-36

1427455_x_at
type: EFO_0005072
part of: A-AFFY-36

Ankrd2 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.00690881934925
p value: 0.0295273623241063
refers to: 1419621_at
refers to: Ankrd2
t statistic: 3.89465459837225

(Sample) diet/normal diet
type: EFO_0000761
property type: diet
property value: normal diet

1415927_at
type: EFO_0005072
part of: A-AFFY-36

Lgals3
type: EnsemblDatabaseReference

Igj
type: EnsemblDatabaseReference

Col1a1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.38738917640119
p value: 0.0145868576542379
refers to: 1423669_at
refers to: Col1a1
t statistic: -4.49141317677407

1450857_a_at
type: EFO_0005072
part of: A-AFFY-36

(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep5
type: Assay
identifier: Gastrocnemius tissue at time 0 from young mouse, biological rep5
has participant: A-AFFY-36

(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep3
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged mouse, biological rep3
has participant: A-AFFY-36

1418822_a_at
type: EFO_0005072
part of: A-AFFY-36

Aplnr
type: EnsemblDatabaseReference

Tpm3
type: EnsemblDatabaseReference

1450196_s_at
type: EFO_0005072
part of: A-AFFY-36

Aplnr
type: EnsemblDatabaseReference

Akt1
type: EnsemblDatabaseReference

Tpm3
type: EnsemblDatabaseReference

Acaa2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.28177376800067
p value: 0.0217880683155556
refers to: Acaa2
refers to: 1455061_a_at
t statistic: -3.81594272577538

Tmem109 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.11279215652714
p value: 0.0199942198183979
refers to: Tmem109
refers to: 1416033_at
t statistic: -3.93433794707455

(Sample) organism part/gastrocnemius muscle
type: UBERON_0001388
property type: organism part
property value: gastrocnemius muscle

Akt1 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.10765813142123
p value: 0.0289029810702971
refers to: 1425711_a_at
refers to: Akt1
t statistic: 3.91628937051033

Gpd1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.18061165268112
p value: 0.0213356399873074
refers to: Gpd1
refers to: 1416204_at
t statistic: -3.84204566011476

Fasn
type: EnsemblDatabaseReference

Gys1
type: EnsemblDatabaseReference

Spop
type: EnsemblDatabaseReference

Spop
type: EnsemblDatabaseReference

A-AFFY-36
type: EFO_0000269

Fasn DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.40063206876991
p value: 0.0395171800243791
refers to: Fasn
refers to: 1423828_at
t statistic: -3.1179575032089

Dusp10
type: EnsemblDatabaseReference

(Sample) organism/Mus musculus
type: Mus musculus
property type: organism
property value: Mus musculus

(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep6
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep6
has participant: A-AFFY-36

Gm21967
type: EnsemblDatabaseReference

1452417_x_at
type: EFO_0005072
part of: A-AFFY-36

Pdha1
type: EnsemblDatabaseReference

1418648_at
type: EFO_0005072
part of: A-AFFY-36

Aplp2
type: EnsemblDatabaseReference

1452308_a_at
type: EFO_0005072
part of: A-AFFY-36

Igj UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.11294944576491
p value: 0.0374237021388449
refers to: Igj
refers to: 1424305_at
t statistic: 3.17344830734402

Scd1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.82419177522543
p value: 0.02860803220399
refers to: Scd1
refers to: 1415965_at
t statistic: -3.46305278691375

Zfp106
type: EnsemblDatabaseReference

(Factor value) AGE/25
type: EFO_0000001
property type: AGE
property value: 25

Col1a1 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.74017921198253
p value: 0.00553575988199005
refers to: Col1a1
refers to: 1423669_at
t statistic: -6.82196386507934

1451666_at
type: EFO_0005072
part of: A-AFFY-36

Chac1
type: EnsemblDatabaseReference

1423669_at
type: EFO_0005072
part of: A-AFFY-36

Analysis of '25 month, normal diet' vs '5 month, normal diet'
type: MicroarrayDifferentialExpressionAnalysis

Snrpn
type: EnsemblDatabaseReference

Snrpn
type: EnsemblDatabaseReference

Speg
type: EnsemblDatabaseReference

Gpd1
type: EnsemblDatabaseReference

Col1a1
type: EnsemblDatabaseReference

1452463_x_at
type: EFO_0005072
part of: A-AFFY-36

1456676_a_at
type: EFO_0005072
part of: A-AFFY-36

Tpm3 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.12390534677836
p value: 0.0368549315026728
refers to: 1449996_a_at
refers to: Tpm3
t statistic: 3.66781718343898

1420816_at
type: EFO_0005072
part of: A-AFFY-36

Camk2a
type: EnsemblDatabaseReference

Anxa6 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.1029193792745
p value: 0.0234890912942597
refers to: 1429246_a_at
refers to: Anxa6
t statistic: -3.7022144533589

1427660_x_at
type: EFO_0005072
part of: A-AFFY-36

Atp1a2
type: EnsemblDatabaseReference

Acly
type: EnsemblDatabaseReference

Col1a2
type: EnsemblDatabaseReference

Igkc UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 2.08912187701431
p value: 0.0110376246847636
refers to: Igkc
refers to: 1427660_x_at
t statistic: 5.06981615932925

Igkc UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 3.05858370593926
p value: 0.00543486692876857
refers to: Igkc
refers to: 1452417_x_at
t statistic: 6.6687474846216

Col1a2
type: EnsemblDatabaseReference

1455061_a_at
type: EFO_0005072
part of: A-AFFY-36

(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep7
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep7
has participant: A-AFFY-36

Acaa2
type: EnsemblDatabaseReference

Rnf14
type: EnsemblDatabaseReference

Ankrd2
type: EnsemblDatabaseReference

1417502_at
type: EFO_0005072
part of: A-AFFY-36

Sln
type: EnsemblDatabaseReference

Col1a2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.08150308743201
p value: 0.0116390965958109
refers to: Col1a2
refers to: 1450857_a_at
t statistic: -4.92422393590425

Col3a1
type: EnsemblDatabaseReference

Sln
type: EnsemblDatabaseReference

Ywhag DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.36160356410041
p value: 0.0236731982759923
refers to: Ywhag
refers to: 1420816_at
t statistic: -3.68685622529548

Amy1
type: EnsemblDatabaseReference

Egln3 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.30371925984345
p value: 0.00553575988199005
refers to: 1418648_at
refers to: Egln3
t statistic: 6.7383879271152

Igkc UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.75300282659809
p value: 0.0299067026438776
refers to: Igkc
refers to: 1452463_x_at
t statistic: 3.41675410380048

(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep4
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged mouse, biological rep4
has participant: A-AFFY-36

1424305_at
type: EFO_0005072
part of: A-AFFY-36

Scd1
type: EnsemblDatabaseReference

(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep1
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged mouse, biological rep1
has participant: A-AFFY-36

Amy1 UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.52012518920966
p value: 0.00282866317625298
refers to: Amy1
refers to: 1417765_a_at
t statistic: 7.98474997206843

Igkc
type: EnsemblDatabaseReference

Arf6 UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.05188197299371
p value: 0.0171908786557763
refers to: 1418822_a_at
refers to: Arf6
t statistic: 4.16880355898478

(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep2
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged mouse, biological rep2
has participant: A-AFFY-36

(Sample) diet/calorie-restricted diet
type: EFO_0000001
property type: diet
property value: calorie-restricted diet

1416525_at
type: EFO_0005072
part of: A-AFFY-36

Egln3
type: EnsemblDatabaseReference

Pfkfb3 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.02342477302215
p value: 0.011944621811497
refers to: 1456676_a_at
refers to: Pfkfb3
t statistic: -4.85885395328026

Capzb DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.12951225863883
p value: 0.019586322420806
refers to: Capzb
refers to: 1453960_a_at
t statistic: -3.96048074071399

Aplp2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.17479541670365
p value: 0.0397716616543484
refers to: Aplp2
refers to: 1421889_a_at
t statistic: -3.10768091832285

Tmem109
type: EnsemblDatabaseReference

Ywhag
type: EnsemblDatabaseReference

Rnf14 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.11918792131155
p value: 0.0182963258892182
refers to: Rnf14
refers to: 1431030_a_at
t statistic: -4.08010194928776

Ighm
type: EnsemblDatabaseReference

Arf6
type: EnsemblDatabaseReference

1451382_at
type: EFO_0005072
part of: A-AFFY-36

(Sample) strain/C57BL/6NHsd
type: EFO_0000001
property type: strain
property value: C57BL/6NHsd

Lgals3
type: EnsemblDatabaseReference

Actc1
type: EnsemblDatabaseReference

Arf6
type: EnsemblDatabaseReference

Anxa6
type: EnsemblDatabaseReference

Igj
type: EnsemblDatabaseReference

Gbp6
type: EnsemblDatabaseReference

(Factor value) DIET/calorie-restricted diet
type: EFO_0000001
property type: DIET
property value: calorie-restricted diet

1429246_a_at
type: EFO_0005072
part of: A-AFFY-36

Pdha1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.02619629501136
p value: 0.0146667610022121
refers to: Pdha1
refers to: 1449137_at
t statistic: -4.43194594820402

1419621_at
type: EFO_0005072
part of: A-AFFY-36

Pfkfb3
type: EnsemblDatabaseReference

1425331_at
type: EFO_0005072
part of: A-AFFY-36

Igkc UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 2.18639439383828
p value: 0.0119227152733862
refers to: 1427455_x_at
refers to: Igkc
t statistic: 4.88095931413263

(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep3
type: Assay
identifier: Gastrocnemius tissue at time 0 from young mouse, biological rep3
has participant: A-AFFY-36

(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep4
type: Assay
identifier: Gastrocnemius tissue at time 0 from young mouse, biological rep4
has participant: A-AFFY-36

Spop DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.16978112746146
p value: 0.0169682476085011
refers to: 1416525_at
refers to: Spop
t statistic: -4.20651999816506

Camk2a DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.14972981522038
p value: 0.0145868576542379
refers to: Camk2a
refers to: 1452453_a_at
t statistic: -4.47429814443356

Fasn
type: EnsemblDatabaseReference

Ncl
type: EnsemblDatabaseReference

(Assay) Gastrocnemius tissue at time 0 from young mouse, biological rep1
type: Assay
identifier: Gastrocnemius tissue at time 0 from young mouse, biological rep1
has participant: A-AFFY-36

Chac1 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.35290282130688
p value: 0.0259180523109166
refers to: Chac1
refers to: 1451382_at
t statistic: -4.05589366586954

1423110_at
type: EFO_0005072
part of: A-AFFY-36

Col1a2 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.73156199647518
p value: 0.00986799686421802
refers to: 1423110_at
refers to: Col1a2
t statistic: -5.50297838846472

1451886_at
type: EFO_0005072
part of: A-AFFY-36

Capzb
type: EnsemblDatabaseReference

Capzb
type: EnsemblDatabaseReference

(Factor value) AGE/5
type: EFO_0000001
property type: AGE
property value: 5

(Sample) age/25
type: EFO_0000001
property type: age
property value: 25

1421889_a_at
type: EFO_0005072
part of: A-AFFY-36

Mus musculus
type: OBI_0100026

1417164_at
type: EFO_0005072
part of: A-AFFY-36

Pdha1
type: EnsemblDatabaseReference

Sln UP in '25 month, normal diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.46271226873772
p value: 0.0415841811689712
refers to: Sln
refers to: 1420884_at
t statistic: 3.0701535186193

Amy1 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.21127847470927
p value: 0.00553575988199005
refers to: 1417765_a_at
refers to: Amy1
t statistic: 7.51178039192207

Aplp2
type: EnsemblDatabaseReference

(Factor value) DIET/normal diet
type: EFO_0000761
property type: DIET
property value: normal diet

1425326_at
type: EFO_0005072
part of: A-AFFY-36

Analysis of '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: MicroarrayDifferentialExpressionAnalysis

1452453_a_at
type: EFO_0005072
part of: A-AFFY-36

Akt1
type: EnsemblDatabaseReference

Col3a1 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.04328923325965
p value: 0.0207686588728281
refers to: Col3a1
refers to: 1427883_a_at
t statistic: -3.88299576151852

Gbp6 UP in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: IncreasedDifferentialExpressionRatio
fold change: 1.08679646456171
p value: 0.0259180523109166
refers to: Gbp6
refers to: 1438676_at
t statistic: 4.0456088899712

Col3a1 DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.42918006760158
p value: 0.00461876576564258
refers to: Col3a1
refers to: 1427883_a_at
t statistic: -7.97734157977011

1420884_at
type: EFO_0005072
part of: A-AFFY-36

Ncl DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.02942352569466
p value: 0.0487277346455857
refers to: Ncl
refers to: 1415773_at
t statistic: -2.90815573911421

Zfp106 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.10489614594316
p value: 0.0219799869420446
refers to: 1425331_at
refers to: Zfp106
t statistic: -3.79662824578853

Chac1
type: EnsemblDatabaseReference

Gys1
type: EnsemblDatabaseReference

(Assay) Gastrocnemius tissue at time 0 from aged mouse, biological rep5
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged mouse, biological rep5
has participant: A-AFFY-36

Dusp10 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.0489052053676
p value: 0.0117869604161867
refers to: 1417164_at
refers to: Dusp10
t statistic: -4.90265511131569

(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep3
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep3
has participant: A-AFFY-36

1425711_a_at
type: EFO_0005072
part of: A-AFFY-36

Speg
type: EnsemblDatabaseReference

Atp1a2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.10682551456327
p value: 0.04244782035502
refers to: Atp1a2
refers to: 1452308_a_at
t statistic: -3.04268373052655

Dusp10
type: EnsemblDatabaseReference

Aplnr DOWN in '25 month, calorie-restricted diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.55600879955213
p value: 0.00754313997711693
refers to: 1438651_a_at
refers to: Aplnr
t statistic: -6.0014255703199

1415773_at
type: EFO_0005072
part of: A-AFFY-36

1426607_at
type: EFO_0005072
part of: A-AFFY-36

Gm21967
type: EnsemblDatabaseReference

1453960_a_at
type: EFO_0005072
part of: A-AFFY-36

E-GEOD-6323
type: microarray 1colour mrna differential
RO_0002162: Mus musculus
description: We investigated age-related changes in the transcriptional profile of skeletal muscle in 5 month old (young) and 25 month old (old) C57BL/6NHsd mice using high density oligonucleotide arrays (22,690 transcripts probed). We identified 712 transcripts that are differentially expressed in young (5 month old) and old (25-month old) mouse skeletal muscle. Caloric restriction (CR) completely or partially reversed 87% of the changes in expression. Examination of individual genes revealed a transcriptional profile indicative of increased p53 activity in the older muscle. To determine whether the increase in p53 activity is associated with transcriptional activation of apoptotic targets, we performed RT-PCR on four well known mediators of p53-induced apoptosis: puma, noxa, tnfrsf10b and bok. Expression levels for these proapoptotic genes increased significantly with age (P<0.05), while CR significantly lowered expression levels for these genes as compared to control fed old mice (P<0.05). Age-related induction of p53-related genes was observed in multiple tissues, but was not observed in SOD2+/- and GPX4+/- mice, suggesting that oxidative stress does not mediate the observed age-related increase in expression. Western blot analysis confirmed that protein levels for both p21 and GADD45a, two established transcriptional targets of p53, were higher in the older muscle tissue. These observations support a role for p53-mediated apoptotic activity in mammalian aging. Experiment Overall Design: Animals were killed by cervical dislocation. Five different animals were used for each experimental group. All collected gastrocnemius tissue was then dissected, placed in a microcentrifuge tube, flash-frozen in liquid nitrogen, and stored at -80ÃÂ°C until processed for microarrays.
uses data from: http://identifiers.org/pubmed/17381838

Atp1a2
type: EnsemblDatabaseReference

Acly
type: EnsemblDatabaseReference

1416033_at
type: EFO_0005072
part of: A-AFFY-36

Zfp106
type: EnsemblDatabaseReference

Col1a2 DOWN in '25 month, normal diet' vs '5 month, normal diet'
type: DecreasedDifferentialExpressionRatio
fold change: -1.38590150266275
p value: 0.0206290799475448
refers to: 1423110_at
refers to: Col1a2
t statistic: -3.8923205150196

Acaa2
type: EnsemblDatabaseReference

Ankrd2
type: EnsemblDatabaseReference

1417765_a_at
type: EFO_0005072
part of: A-AFFY-36

(Assay) Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep5
type: Assay
identifier: Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep5
has participant: A-AFFY-36