Fam63a DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Actb DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ide DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Hnf4a DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Apob DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Habp2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pdcd6ip DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Gpd1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Acox1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Rnf7 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Lyz2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Thoc1 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Glrx DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Myeov2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ctla2b UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Cyb5r3 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pcmt1 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Nbr1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Aplp2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ahnak UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Mrpl41 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Sec61a1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
S100a10 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Gpcpd1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Fabp2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Abhd17b UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
(Factor value) ORGANISM_PART/quadriceps femoris
Eif4g1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Gpd2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Cetn2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ndst1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Rapgef4 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Iigp1 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'quadriceps femoris'
Ormdl1 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Prox1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Baiap2l1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Hdlbp DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Atp6v0a1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Slc7a2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ptprf DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Wfdc17 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Rgs16 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Hspa9 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Fam96a UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Entpd5 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
A1cf DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Nedd4 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Dsg2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Hmbs UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Sel1l DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Nxt2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Anapc10 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Znhit3 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Mal2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Keap1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Smim4 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Papss2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Guk1 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
(Factor value) GENOTYPE/wild type genotype
Mmp19 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
PGC1beta is a transcriptional coactivator that potently stimulates mitochondrial biogenesis and respiration of cells. Here, we have generated mice lacking exons 3 to 4 of the Pgc1beta gene (PGC1beta E3,4-/E3,4- mice). These mice express a mutant protein that has reduced coactivation activity on a subset of transcription factors, including ERRalpha, a major target of PGC1beta in the induction of mitochondrial gene expression. The mutant mice have reduced expression of OXPHOS genes and mitochondrial dysfunction in liver and skeletal muscle as well as elevated liver triglycerides. Euglycemic-hyperinsulinemic clamp and insulin signaling studies show that PGC1beta mutant mice have normal skeletal muscle response to insulin, but have hepatic insulin resistance. These results demonstrate that PGC1beta is required for normal expression of OXPHOS genes and mitochondrial function in liver and skeletal muscle. Importantly, these abnormalities do not cause insulin resistance in skeletal muscle but cause substantially reduced insulin action in the liver. Experiment Overall Design: Gene expression levels in liver tissue and quadriceps muscle were compared between WT/Control and PGC1beta mutant tissue. Total RNA was extracted from liver and skeletal muscle using RNAeasy kit (Qiagen, Valencia, CA), according to the manufacturerÃÂ¢ÃÂÃÂs instructions. Synthesis of cRNA, hybridization and scanning of the Affymetrix Murine 430 2.0 chip was performed by Dana Farber Cancer Institute Microarray Core Facility. The microarray data was analyzed by Clustering Analysis using the d-Chip software (Li and Wong, 2001).
Ak3 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pex6 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Isoc1 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'quadriceps femoris'
Rmnd5a DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Leap2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Analysis of 'PGC-1beta mutant' vs 'wild type genotype' in 'quadriceps femoris'
Chac2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Tgds UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Myoz3 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'quadriceps femoris'
Slc10a2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Abcc2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Akr1c20 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Atp6v0b UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Plac8 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Txndc17 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ube4a DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pcx DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Dynlt3 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Cmah DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Gm10658 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Mrpl13 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Aim1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
(Factor value) GENOTYPE/PGC-1beta mutant
Sf3b6 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Snrpe UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Saa4 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Analysis of 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Sec11c UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Acyp1 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Golph3 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Zfp386 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pla2g6 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Egfr DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
C1d UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Slc39a14 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pcyt1a DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Dennd5b DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Rps9 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Sppl2a DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Rmdn2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Shc1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Abcb10 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Gpt2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Tyrobp UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pdxdc1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Nrip1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Itgb3bp UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Nsmce1 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ywhag DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Kpnb1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
9630013D21Rik UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Lpp DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Fkbp3 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Atp11c DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Nrep UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Arel1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Acsl1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Myo1c DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Psmc6 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Sgpl1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Wbp1l DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Gna12 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Zfp931 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pex5 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ndufaf5 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Tmsb4x UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Igf2r DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Gdpd3 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Errfi1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ndufaf6 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Cyp2c70 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Bud31 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
(Sample) organism part/quadriceps femoris
Cyp4a10 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Mtus1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Cnpy2 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Fbxw11 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Epas1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ncoa2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Rnf4 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pfdn5 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Igfbp4 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Mbnl2 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Tgoln1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Dnajc15 UP in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Cyp7a1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Twsg1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Lman1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Cnnm3 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Pck1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Prlr DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Slco2a1 DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'
Ptprk DOWN in 'PGC-1beta mutant' vs 'wild type genotype' in 'liver'