AT2G47200 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G07690 DOWN in 'cngc4 mutant' vs 'wild type'
ASN1 UP in 'cngc4 mutant' vs 'wild type'
AT3G59370 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G80440 UP in 'cngc4 mutant' vs 'wild type'
ATAGP30 DOWN in 'cngc4 mutant' vs 'wild type'
AT5G64080 DOWN in 'cngc4 mutant' vs 'wild type'
PAP7 DOWN in 'cngc4 mutant' vs 'wild type'
PER10 UP in 'cngc1 mutant' vs 'wild type'
XTH11 UP in 'cngc4 mutant' vs 'wild type'
AT1G14890 UP in 'cngc4 mutant' vs 'wild type'
AT1G80180 UP in 'cngc4 mutant' vs 'wild type'
CYP71B3 UP in 'cngc4 mutant' vs 'wild type'
AT1G33055 UP in 'cngc4 mutant' vs 'wild type'
ATST2A UP in 'cngc4 mutant' vs 'wild type'
ATJ11 UP in 'cngc4 mutant' vs 'wild type'
AT1G54740 UP in 'cngc4 mutant' vs 'wild type'
AT4G03500 DOWN in 'cngc4 mutant' vs 'wild type'
(Assay) CH001_ATH1_A009-Hampt-wsc_repeat
AT3G29240 UP in 'cngc4 mutant' vs 'wild type'
XTR8 DOWN in 'cngc4 mutant' vs 'wild type'
AT4G24140 DOWN in 'cngc4 mutant' vs 'wild type'
AT2G15960 UP in 'cngc4 mutant' vs 'wild type'
AT1G21680 UP in 'cngc4 mutant' vs 'wild type'
MAX2 UP in 'cngc4 mutant' vs 'wild type'
SKP2A UP in 'cngc4 mutant' vs 'wild type'
CLB6 UP in 'cngc4 mutant' vs 'wild type'
BGAL8 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G72230 DOWN in 'cngc4 mutant' vs 'wild type'
ATCSLE1 UP in 'cngc4 mutant' vs 'wild type'
DIN4 UP in 'cngc4 mutant' vs 'wild type'
XTH9 DOWN in 'cngc4 mutant' vs 'wild type'
ATMKK9 UP in 'cngc4 mutant' vs 'wild type'
BCDH BETA1 UP in 'cngc4 mutant' vs 'wild type'
AT2G46750 UP in 'cngc4 mutant' vs 'wild type'
MCCB UP in 'cngc4 mutant' vs 'wild type'
AT2G05540 UP in 'cngc4 mutant' vs 'wild type'
AGP13 DOWN in 'cngc4 mutant' vs 'wild type'
AT4G33710 UP in 'cngc4 mutant' vs 'wild type'
RAP2.3 UP in 'cngc4 mutant' vs 'wild type'
AGT3 UP in 'cngc4 mutant' vs 'wild type'
ATTPS8 UP in 'cngc4 mutant' vs 'wild type'
C2H2 UP in 'cngc4 mutant' vs 'wild type'
AT1G05340 UP in 'cngc4 mutant' vs 'wild type'
AT1G76590 UP in 'cngc4 mutant' vs 'wild type'
MEE14 UP in 'cngc4 mutant' vs 'wild type'
ATL8 UP in 'cngc4 mutant' vs 'wild type'
SEN1 UP in 'cngc4 mutant' vs 'wild type'
XTH7 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G12080 DOWN in 'cngc4 mutant' vs 'wild type'
TMT2 DOWN in 'cngc1 mutant' vs 'wild type'
DIN11 UP in 'cngc4 mutant' vs 'wild type'
AT1G27090 DOWN in 'cngc1 mutant' vs 'wild type'
ARI5 UP in 'cngc4 mutant' vs 'wild type'
ATTPS11 UP in 'cngc4 mutant' vs 'wild type'
AT2G35820 UP in 'cngc4 mutant' vs 'wild type'
RAP2.2 UP in 'cngc4 mutant' vs 'wild type'
AT1G23390 UP in 'cngc4 mutant' vs 'wild type'
FAR4 DOWN in 'cngc4 mutant' vs 'wild type'
AT2G33830 UP in 'cngc4 mutant' vs 'wild type'
ADF9 UP in 'cngc4 mutant' vs 'wild type'
AT2G01610 DOWN in 'cngc4 mutant' vs 'wild type'
AT2G16005 DOWN in 'cngc1 mutant' vs 'wild type'
Analysis of 'cngc4 mutant' vs 'wild type'
PEX11D UP in 'cngc4 mutant' vs 'wild type'
VAS DOWN in 'cngc4 mutant' vs 'wild type'
AT4G36610 DOWN in 'cngc4 mutant' vs 'wild type'
PER20 DOWN in 'cngc4 mutant' vs 'wild type'
WRKY26 UP in 'cngc4 mutant' vs 'wild type'
ALDH7B4 UP in 'cngc4 mutant' vs 'wild type'
SAG20 UP in 'cngc4 mutant' vs 'wild type'
PER11 DOWN in 'cngc4 mutant' vs 'wild type'
AT4G11190 DOWN in 'cngc4 mutant' vs 'wild type'
ATOSM34 UP in 'cngc4 mutant' vs 'wild type'
AT3G25790 DOWN in 'cngc4 mutant' vs 'wild type'
AT5G04120 DOWN in 'cngc4 mutant' vs 'wild type'
LKR/SDH UP in 'cngc4 mutant' vs 'wild type'
TMT2 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G64590 DOWN in 'cngc4 mutant' vs 'wild type'
AAP3 UP in 'cngc4 mutant' vs 'wild type'
GH3.17 DOWN in 'cngc4 mutant' vs 'wild type'
CRRSP5 UP in 'cngc4 mutant' vs 'wild type'
HSPRO2 UP in 'cngc4 mutant' vs 'wild type'
PER70 UP in 'cngc4 mutant' vs 'wild type'
LAC7 UP in 'cngc4 mutant' vs 'wild type'
(Sample) developmental stage/Boyes key 3.9
PR-1-LIKE DOWN in 'cngc4 mutant' vs 'wild type'
AT5G12420 DOWN in 'cngc4 mutant' vs 'wild type'
AT2G19970 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G80160 UP in 'cngc4 mutant' vs 'wild type'
PGP21 UP in 'cngc4 mutant' vs 'wild type'
AtOCT4 UP in 'cngc4 mutant' vs 'wild type'
AT3G10020 UP in 'cngc4 mutant' vs 'wild type'
SDR4 DOWN in 'cngc4 mutant' vs 'wild type'
ATCTH UP in 'cngc4 mutant' vs 'wild type'
GRP3S UP in 'cngc4 mutant' vs 'wild type'
AT4G37180 UP in 'cngc4 mutant' vs 'wild type'
RD26 UP in 'cngc4 mutant' vs 'wild type'
STP14 UP in 'cngc4 mutant' vs 'wild type'
WRKY45 UP in 'cngc4 mutant' vs 'wild type'
AT4G28040 UP in 'cngc4 mutant' vs 'wild type'
KCS2 DOWN in 'cngc4 mutant' vs 'wild type'
AT2G46220 UP in 'cngc4 mutant' vs 'wild type'
ATBCAT-2 UP in 'cngc4 mutant' vs 'wild type'
AT1G78990 DOWN in 'cngc4 mutant' vs 'wild type'
PER10 UP in 'cngc4 mutant' vs 'wild type'
AT4G15610 UP in 'cngc4 mutant' vs 'wild type'
ATBCA6 UP in 'cngc4 mutant' vs 'wild type'
ATPRP1 UP in 'cngc1 mutant' vs 'wild type'
SKIP20 UP in 'cngc4 mutant' vs 'wild type'
UGE1 UP in 'cngc4 mutant' vs 'wild type'
AT2G31945 UP in 'cngc4 mutant' vs 'wild type'
AT1G09750 DOWN in 'cngc4 mutant' vs 'wild type'
ATPSK4 UP in 'cngc4 mutant' vs 'wild type'
GASA5 DOWN in 'cngc4 mutant' vs 'wild type'
AT3G05950 UP in 'cngc4 mutant' vs 'wild type'
AT2G33850 DOWN in 'cngc4 mutant' vs 'wild type'
AT2G21560 UP in 'cngc4 mutant' vs 'wild type'
Analysis of 'cngc1 mutant' vs 'wild type'
AT2G22880 UP in 'cngc4 mutant' vs 'wild type'
AT1G56220 UP in 'cngc4 mutant' vs 'wild type'
CYP72A15 UP in 'cngc4 mutant' vs 'wild type'
ATMGL UP in 'cngc4 mutant' vs 'wild type'
(Assay) CH001_ATH1_A003-Hampt-c4a_repeat
AT1G19530 UP in 'cngc4 mutant' vs 'wild type'
CP12-1 UP in 'cngc4 mutant' vs 'wild type'
PPDK UP in 'cngc4 mutant' vs 'wild type'
ATNAC3 UP in 'cngc4 mutant' vs 'wild type'
AtbZIP5 DOWN in 'cngc4 mutant' vs 'wild type'
DYL1 UP in 'cngc4 mutant' vs 'wild type'
AT4G38470 UP in 'cngc4 mutant' vs 'wild type'
AT4G33980 UP in 'cngc4 mutant' vs 'wild type'
AT3G16150 UP in 'cngc4 mutant' vs 'wild type'
HIS1-3 UP in 'cngc4 mutant' vs 'wild type'
CYP72A13 UP in 'cngc4 mutant' vs 'wild type'
PER12 UP in 'cngc4 mutant' vs 'wild type'
UGT73B1 UP in 'cngc4 mutant' vs 'wild type'
AT4G27450 UP in 'cngc4 mutant' vs 'wild type'
AT4G29190 UP in 'cngc4 mutant' vs 'wild type'
AT2G15695 UP in 'cngc4 mutant' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
SCPL7 UP in 'cngc4 mutant' vs 'wild type'
AT4G33550 DOWN in 'cngc4 mutant' vs 'wild type'
ATPSK2 UP in 'cngc4 mutant' vs 'wild type'
ATSCO1 UP in 'cngc4 mutant' vs 'wild type'
ATKTI1 UP in 'cngc4 mutant' vs 'wild type'
AT3G22620 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G21100 UP in 'cngc4 mutant' vs 'wild type'
SRK2C UP in 'cngc4 mutant' vs 'wild type'
AGP9 DOWN in 'cngc4 mutant' vs 'wild type'
XTH8 DOWN in 'cngc4 mutant' vs 'wild type'
DIN2 UP in 'cngc4 mutant' vs 'wild type'
ATNRT2:1 DOWN in 'cngc4 mutant' vs 'wild type'
PETC UP in 'cngc4 mutant' vs 'wild type'
AT3G05950 UP in 'cngc1 mutant' vs 'wild type'
(Assay) CH001_ATH1_A005-Hampt-wsb_repeat
GLTP3 UP in 'cngc4 mutant' vs 'wild type'
AT1G05575 UP in 'cngc4 mutant' vs 'wild type'
AT2G45210 UP in 'cngc4 mutant' vs 'wild type'
ATPP2-A9 DOWN in 'cngc4 mutant' vs 'wild type'
LAC3 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G25400 UP in 'cngc4 mutant' vs 'wild type'
ATEXLB1 UP in 'cngc4 mutant' vs 'wild type'
SOUL-1 UP in 'cngc4 mutant' vs 'wild type'
AGP31 DOWN in 'cngc4 mutant' vs 'wild type'
AT4G38540 UP in 'cngc4 mutant' vs 'wild type'
LCR78 UP in 'cngc4 mutant' vs 'wild type'
AT3G15650 DOWN in 'cngc4 mutant' vs 'wild type'
CASP2 DOWN in 'cngc4 mutant' vs 'wild type'
MCCA UP in 'cngc4 mutant' vs 'wild type'
SNG1 UP in 'cngc4 mutant' vs 'wild type'
AT3G05150 UP in 'cngc4 mutant' vs 'wild type'
pEARLI 1 UP in 'cngc4 mutant' vs 'wild type'
AT2G22510 DOWN in 'cngc4 mutant' vs 'wild type'
ATHCHIB UP in 'cngc4 mutant' vs 'wild type'
AT1G64160 UP in 'cngc4 mutant' vs 'wild type'
THA1 UP in 'cngc4 mutant' vs 'wild type'
AT1G74460 DOWN in 'cngc4 mutant' vs 'wild type'
PLP2 UP in 'cngc4 mutant' vs 'wild type'
NIA1 DOWN in 'cngc4 mutant' vs 'wild type'
AT4G16260 UP in 'cngc4 mutant' vs 'wild type'
Background: Release of the caesium radioisotope 137Cs during weapons testing and industrial activity has contaminated thousands of hectares of agricultural land. Ingesting 137Cs has damaging and, sometimes, fatal effects. Most Cs enters the food chain through plants. The generation of _safe_ crops that exclude Cs and can be cultivated on contaminated land requires knowledge about the mechanisms for Cs uptake. Caesium is chemically similar to potassium (K) and might enter plants through K+ transporters in the plasma membrane of root cells. To determine which transporters mediate Cs entry to plants, we have compared the accumulation of Cs and K by wildtype Arabidopsis with mutants lacking specific K+ transporters. Preliminary results showed that Cs concentration in the shoots of akt1-1, cngc1 and cngc4 (obtained from the Wisconsin T-DNA knockout facility) differed significantly from the Wassilewskija wildtype (Ws-2). A cursory investigation of their transcriptome, using the Affymetrix Arabidopsis 8K GeneChip, showed that the expression of several genes encoding K+ transporters differed between mutants and wildtype plants. The aim of this GarNet project is to confirm the previous observations and to identify further genes that are differentially expressed in mutant and wildtype plants and which might impact on Cs accumulation. Methods: Arabidopsis mutants akt1-1 (N3762), cngc1 and cngc4 and their parental ecotype Wassilewskija -2 (N1601) will be sown on MS agar and transferred to hydroponics 21 days after germination. Seedlings will be grown for a further 7 days on full nutrient solution under continuous light in a Saxcil growth cabinet. RNA will be extracted from roots of mutant and parent (control) plants at the same growth stage and twelve complete-genome Affymetrix GeneChips (3 biological replicates of material from wildtype and 3 mutants) are requested to determine the differences in their transcriptome under comparable environmental conditions. Experiment Overall Design: Number of plants pooled:20-40
SVB DOWN in 'cngc4 mutant' vs 'wild type'
AT1G19960 UP in 'cngc4 mutant' vs 'wild type'
AT5G66815 DOWN in 'cngc4 mutant' vs 'wild type'
ATEXPB2 DOWN in 'cngc4 mutant' vs 'wild type'
ORA59 UP in 'cngc4 mutant' vs 'wild type'
BCE2 UP in 'cngc4 mutant' vs 'wild type'
GAPA UP in 'cngc4 mutant' vs 'wild type'
GDU1 UP in 'cngc4 mutant' vs 'wild type'
UGE3 UP in 'cngc4 mutant' vs 'wild type'
AT1G20070 DOWN in 'cngc4 mutant' vs 'wild type'
AT4G25250 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G15040 UP in 'cngc4 mutant' vs 'wild type'
PER38 UP in 'cngc4 mutant' vs 'wild type'
AtbZIP UP in 'cngc4 mutant' vs 'wild type'
ATJRG21 UP in 'cngc4 mutant' vs 'wild type'
SDR4 DOWN in 'cngc1 mutant' vs 'wild type'
MERI5B UP in 'cngc4 mutant' vs 'wild type'
CCOAMT UP in 'cngc4 mutant' vs 'wild type'
AT3G27400 DOWN in 'cngc4 mutant' vs 'wild type'
MT2A UP in 'cngc4 mutant' vs 'wild type'
AT2G20670 UP in 'cngc4 mutant' vs 'wild type'
AT1G76520 UP in 'cngc4 mutant' vs 'wild type'
AT1G03820 DOWN in 'cngc4 mutant' vs 'wild type'
AT1G10140 UP in 'cngc4 mutant' vs 'wild type'
LOX1 UP in 'cngc4 mutant' vs 'wild type'
AT3G26510 UP in 'cngc4 mutant' vs 'wild type'
PDS1 UP in 'cngc4 mutant' vs 'wild type'
AT2G38600 DOWN in 'cngc4 mutant' vs 'wild type'
HIR3 UP in 'cngc4 mutant' vs 'wild type'
AT5G14120 UP in 'cngc4 mutant' vs 'wild type'
GUT15 UP in 'cngc4 mutant' vs 'wild type'
ATBCB UP in 'cngc4 mutant' vs 'wild type'
AT3G13310 UP in 'cngc4 mutant' vs 'wild type'
ATEXPA17 DOWN in 'cngc4 mutant' vs 'wild type'