(Assay) LacZ treated 0610_Dm_S2_LacZ-treated_1
(Sample) organism/Drosophila melanogaster
CG3303 DOWN in 'NELF-B and NELF-E' vs 'none'
fend DOWN in 'NELF-B and NELF-E' vs 'none'
CG5399 DOWN in 'NELF-B and NELF-E' vs 'none'
NELF-B DOWN in 'NELF-B and NELF-E' vs 'none'
CG1516 DOWN in 'NELF-B and NELF-E' vs 'none'
QC DOWN in 'NELF-B and NELF-E' vs 'none'
dream DOWN in 'NELF-B and NELF-E' vs 'none'
CG14642 DOWN in 'NELF-B and NELF-E' vs 'none'
CG14273 DOWN in 'NELF-B and NELF-E' vs 'none'
CG1358 DOWN in 'NELF-B and NELF-E' vs 'none'
GstD5 UP in 'NELF-B and NELF-E' vs 'none'
Npc2b DOWN in 'NELF-B and NELF-E' vs 'none'
Cyp4d20 DOWN in 'NELF-B and NELF-E' vs 'none'
Tsp42El DOWN in 'NELF-B and NELF-E' vs 'none'
CG8008 DOWN in 'NELF-B and NELF-E' vs 'none'
ClC-a DOWN in 'NELF-B and NELF-E' vs 'none'
Hexo2 DOWN in 'NELF-B and NELF-E' vs 'none'
(Assay) LacZ treated 0610_Dm_S2_LacZ-treated_3
CG6231 DOWN in 'NELF-B and NELF-E' vs 'none'
scaf UP in 'NELF-B and NELF-E' vs 'none'
Tequila DOWN in 'NELF-B and NELF-E' vs 'none'
CG43103 DOWN in 'NELF-B and NELF-E' vs 'none'
CG6776 DOWN in 'NELF-B and NELF-E' vs 'none'
Tl DOWN in 'NELF-B and NELF-E' vs 'none'
CG10026 DOWN in 'NELF-B and NELF-E' vs 'none'
CG30456 DOWN in 'NELF-B and NELF-E' vs 'none'
CG18557 DOWN in 'NELF-B and NELF-E' vs 'none'
Adgf-A DOWN in 'NELF-B and NELF-E' vs 'none'
drpr DOWN in 'NELF-B and NELF-E' vs 'none'
PGRP-SA DOWN in 'NELF-B and NELF-E' vs 'none'
(Assay) Untreated 0610_Dm_S2_untreated_ 2
(Assay) NELF depleted 0610_Dm_S2_NELF-depleted_3
NELF depleted 0610_Dm_S2_NELF-depleted_3
btn DOWN in 'NELF-B and NELF-E' vs 'none'
Cyp12a4 DOWN in 'NELF-B and NELF-E' vs 'none'
Esyt2 DOWN in 'NELF-B and NELF-E' vs 'none'
CG8306 DOWN in 'NELF-B and NELF-E' vs 'none'
Spn77Ba DOWN in 'NELF-B and NELF-E' vs 'none'
numb DOWN in 'NELF-B and NELF-E' vs 'none'
ImpE3 DOWN in 'NELF-B and NELF-E' vs 'none'
CG34328 UP in 'NELF-B and NELF-E' vs 'none'
Nf1 DOWN in 'NELF-B and NELF-E' vs 'none'
Gs1 DOWN in 'NELF-B and NELF-E' vs 'none'
(Assay) NELF depleted 0610_Dm_S2_NELF-depleted_2
NELF depleted 0610_Dm_S2_NELF-depleted_2
GstD1 UP in 'NELF-B and NELF-E' vs 'none'
(Factor value) RNA_INTERFERENCE/NELF-B and NELF-E
CG14291 DOWN in 'NELF-B and NELF-E' vs 'none'
Analysis of 'NELF-B and NELF-E' vs 'none'
CG5888 DOWN in 'NELF-B and NELF-E' vs 'none'
(Assay) LacZ treated 0610_Dm_S2_LacZ-treated_2
cu DOWN in 'NELF-B and NELF-E' vs 'none'
CG14629 DOWN in 'NELF-B and NELF-E' vs 'none'
CG6912 DOWN in 'NELF-B and NELF-E' vs 'none'
Jheh1 DOWN in 'NELF-B and NELF-E' vs 'none'
Ugt35a DOWN in 'NELF-B and NELF-E' vs 'none'
CG1998 DOWN in 'NELF-B and NELF-E' vs 'none'
CG11400 DOWN in 'NELF-B and NELF-E' vs 'none'
Mmp2 DOWN in 'NELF-B and NELF-E' vs 'none'
Ugt58Fa DOWN in 'NELF-B and NELF-E' vs 'none'
Fmo-2 DOWN in 'NELF-B and NELF-E' vs 'none'
CG9008 UP in 'NELF-B and NELF-E' vs 'none'
eater DOWN in 'NELF-B and NELF-E' vs 'none'
MFS10 DOWN in 'NELF-B and NELF-E' vs 'none'
Oat DOWN in 'NELF-B and NELF-E' vs 'none'
pgant2 DOWN in 'NELF-B and NELF-E' vs 'none'
CG34232 DOWN in 'NELF-B and NELF-E' vs 'none'
Cyp12a5 DOWN in 'NELF-B and NELF-E' vs 'none'
CG32521 DOWN in 'NELF-B and NELF-E' vs 'none'
CG2247 UP in 'NELF-B and NELF-E' vs 'none'
mfas UP in 'NELF-B and NELF-E' vs 'none'
CG11739 DOWN in 'NELF-B and NELF-E' vs 'none'
CG15784 UP in 'NELF-B and NELF-E' vs 'none'
mnd DOWN in 'NELF-B and NELF-E' vs 'none'
Kal1 DOWN in 'NELF-B and NELF-E' vs 'none'
CG14523 DOWN in 'NELF-B and NELF-E' vs 'none'
CG2930 DOWN in 'NELF-B and NELF-E' vs 'none'
regucalcin DOWN in 'NELF-B and NELF-E' vs 'none'
Swim UP in 'NELF-B and NELF-E' vs 'none'
CG13907 DOWN in 'NELF-B and NELF-E' vs 'none'
CG13397 DOWN in 'NELF-B and NELF-E' vs 'none'
Hsp70Bc UP in 'NELF-B and NELF-E' vs 'none'
yellow-f DOWN in 'NELF-B and NELF-E' vs 'none'
(Factor value) RNA_INTERFERENCE/beta-galactosidase (mock-treated)
CG11395 DOWN in 'NELF-B and NELF-E' vs 'none'
GNBP3 DOWN in 'NELF-B and NELF-E' vs 'none'
CG34417 DOWN in 'NELF-B and NELF-E' vs 'none'
egr DOWN in 'NELF-B and NELF-E' vs 'none'
CecB DOWN in 'NELF-B and NELF-E' vs 'none'
CG31274 DOWN in 'NELF-B and NELF-E' vs 'none'
Idgf3 DOWN in 'NELF-B and NELF-E' vs 'none'
CG9338 DOWN in 'NELF-B and NELF-E' vs 'none'
(Assay) Untreated 0610_Dm_S2_untreated_3
miple2 DOWN in 'NELF-B and NELF-E' vs 'none'
wun2 DOWN in 'NELF-B and NELF-E' vs 'none'
betaTub97EF DOWN in 'NELF-B and NELF-E' vs 'none'
CG13795 DOWN in 'NELF-B and NELF-E' vs 'none'
pirk DOWN in 'NELF-B and NELF-E' vs 'none'
p38c DOWN in 'NELF-B and NELF-E' vs 'none'
Cyp310a1 DOWN in 'NELF-B and NELF-E' vs 'none'
(Assay) Untreated 0610_Dm_S2_untreated_1
CG10877 DOWN in 'NELF-B and NELF-E' vs 'none'
TepII DOWN in 'NELF-B and NELF-E' vs 'none'
Spn28D DOWN in 'NELF-B and NELF-E' vs 'none'
CG10824 DOWN in 'NELF-B and NELF-E' vs 'none'
CG10962 DOWN in 'NELF-B and NELF-E' vs 'none'
PGRP-SD DOWN in 'NELF-B and NELF-E' vs 'none'
(Assay) NELF depleted 0610_Dm_S2_NELF-depleted_1
NELF depleted 0610_Dm_S2_NELF-depleted_1
CG6310 DOWN in 'NELF-B and NELF-E' vs 'none'
Hsp26 UP in 'NELF-B and NELF-E' vs 'none'
CG12133 DOWN in 'NELF-B and NELF-E' vs 'none'
Syx4 DOWN in 'NELF-B and NELF-E' vs 'none'
fat-spondin DOWN in 'NELF-B and NELF-E' vs 'none'
Eip74EF DOWN in 'NELF-B and NELF-E' vs 'none'
CG16985 DOWN in 'NELF-B and NELF-E' vs 'none'
sdt DOWN in 'NELF-B and NELF-E' vs 'none'
CG11163 DOWN in 'NELF-B and NELF-E' vs 'none'
CG14253 DOWN in 'NELF-B and NELF-E' vs 'none'
CG13117 DOWN in 'NELF-B and NELF-E' vs 'none'
To determine the physiological targets of the NELF complex, and provide insight into the mechanism of NELF activity in vivo. Experiment Overall Design: Drosophila melanogaster S2 cells, obtained from the Drosophila Genomics Resource Center, were untreated, or treated with dsRNA for 72 hours, as described in Armknecht, S. et al. (2005) Methods in Enzymology, 392: pp. 55-73. Total RNA was then extracted using the RNeasy RNA extraction kit, with on column DNAse digestion (Qiagen), according to manufacturerÃÂ¢ÃÂÃÂs protocol. Gene expression analysis was conducted using Drosophila Genome 2.0 GenechipÃÂÃÂ® arrays (Affymetrix, Santa Clara, CA). Starting with 1ug of total RNA, biotin-labeled cRNA was produced using the Affymetrix 3ÃÂ¢ÃÂÃÂ Amplification One-Cycle Target labeling kit according to manufacturerÃÂ¢ÃÂÃÂs protocol. For each array, 10ug of amplified cRNAs were fragmented and hybridized to the array for 16 hours in a rotating hybridization oven using the Affymetrix Eukaryotic Target Hybridization Controls and protocol. Slides were stained and washed as indicated in the Antibody Amplification Stain for Eukaryotic Targets protocol using the Affymetrix Fluidics Station FS450. Arrays were then scanned with an Affymetrix Scanner 3000 and data was obtained using the GenechipÃÂÃÂ® Operating Software (Version 1.2.0.037) and imported into the Rosetta Resolver system (Version 6.0).
CG12868 UP in 'NELF-B and NELF-E' vs 'none'
CG14869 DOWN in 'NELF-B and NELF-E' vs 'none'