AT1G24140 UP in 'fumonisin B1' vs 'control'
AT1G19020 UP in 'fumonisin B1' vs 'control'
AT3G22600 UP in 'fumonisin B1' vs 'control'
AT2G32190 UP in 'fumonisin B1' vs 'control'
ATHPP2C5 UP in 'fumonisin B1' vs 'control'
AT1G56060 UP in 'fumonisin B1' vs 'control'
AT3G53600 UP in 'fumonisin B1' vs 'control'
AT4G01700 DOWN in 'fumonisin B1' vs 'control'
AT3G48640 DOWN in 'fumonisin B1' vs 'control'
AT3G13650/MMM17_4 UP in 'fumonisin B1' vs 'control'
ATSAR1 UP in 'fumonisin B1' vs 'control'
AT1G50590 UP in 'fumonisin B1' vs 'control'
CYP71B22 UP in 'fumonisin B1' vs 'control'
AT2G28710 UP in 'fumonisin B1' vs 'control'
AT2G39400 UP in 'fumonisin B1' vs 'control'
BGLU11 DOWN in 'fumonisin B1' vs 'control'
AT1G63220 UP in 'fumonisin B1' vs 'control'
PLA-I{beta]2 UP in 'fumonisin B1' vs 'control'
AT3G60540 UP in 'fumonisin B1' vs 'control'
WRKY54 DOWN in 'fumonisin B1' vs 'control'
ATACA2 UP in 'fumonisin B1' vs 'control'
AT2G22620 UP in 'fumonisin B1' vs 'control'
AT1G29310 UP in 'fumonisin B1' vs 'control'
F6'H1 DOWN in 'fumonisin B1' vs 'control'
AT1G26380 UP in 'fumonisin B1' vs 'control'
ATERF6 UP in 'fumonisin B1' vs 'control'
UGT89C1 DOWN in 'fumonisin B1' vs 'control'
AT1G51850 DOWN in 'fumonisin B1' vs 'control'
WRKY70 DOWN in 'fumonisin B1' vs 'control'
AT3G60420 DOWN in 'fumonisin B1' vs 'control'
anac042 UP in 'fumonisin B1' vs 'control'
AT2G23910 DOWN in 'fumonisin B1' vs 'control'
(Factor value) GROWTH_CONDITION/fumonisin B1
PCD is a highly organised process that is involved in development and in an organisms response to biotic stresses (toxins and avirulent pathogens) and abiotic stresses (such as temperature, water availability, etc.). It is a genetically regulated form of cellular suicide, however in plants the underlying process is poorly understood. Although PCD may occur in response to different stimuli; we believe once it is triggered, one core mechanism is responsible for the cellular demise. It is our aim to identify the elements of this mechanism. We will do this by expanding on the work of a previous user of GARNet's GeneChip microarray facility, Dr. Jodi Swidzinski. She utilised an Arabidopsis cell suspension system; performing microarray analysis on both senescing, and heat shock induced PCD samples. This resulted in data showing that a large number of genes were upregulated (or downregulated) in response to both treatments. We are working under the premise that some of the genes that were similarly regulated under both treatments must be core PCD genes. However because of the large amount of data generated it is difficult to choose appropriate candidate genes (with any degree of confidence that they are core genes) for further study. We propose to use a third PCD-inducing treatment, involving a mycotoxin, to generate another population of microarray results. The mycotoxin we intend to use is Fumonisin B1 (FB1). This is an extremely potent compound that induces PCD by disrupting ceramide synthesis. We have found Arabidopsis protoplasts to be much more sensitive than cells to the toxin at low concentrations. Protoplasts are treated with 20mM FB1 and RNA is extracted at time points when 0%, 20% and 40% of protoplasts have died. This RNA is then pooled. RNA from methanol treated protoplasts is used as a control. We intend for these RNA samples to be subjected to GeneChip microarray analysis. This would identify genes differentially regulated due to FB1 treatment, which would be interesting in itself. However, by combining this data with that from previous work by Swidzinski (2002) we will be able to decrease the pool of possible core genes further, and increase the chances of selecting an appropriate candidate gene. This will both improve upon existing work and add value to an existing GARNet data set. Experimenter name = Mark Diamond; Experimenter phone = 017162251; Experimenter fax = 017161153; Experimenter address = Botany Department; Experimenter address = University College Dublin; Experimenter address = Belfield; Experimenter zip/postal_code = Dublin 4; Experimenter country = Ireland Experiment Overall Design: 8 samples were used in this experiment
JAZ7 DOWN in 'fumonisin B1' vs 'control'