CSF2RA DOWN in 'rosiglitazone' vs 'none'
CDC42EP3 UP in 'rosiglitazone' vs 'none'
KIAA1161 DOWN in 'rosiglitazone' vs 'none'
ATP2B1 DOWN in 'rosiglitazone' vs 'none'
CLEC7A DOWN in 'rosiglitazone' vs 'none'
Our data indicated that activation of the PPARg nuclear receptor induces a retinoid response in human dendritic cells. In order to assess the contribution of retinoid signaling to the PPARg response we decided to use a combination of pharmacological activators and inhibitors of these pathways. Cells were treated with the synthetic PPARg ligand rosiglitazone (RSG), or with RSG along with the RARa antagonist (AGN193109) to block RARa mediated gene expression, or the RARa specific agonists (AM580) alone. This design allows one to determine if retinoid signaling is a downstream event of PPARg activation and what portion of PPARg regulated genes are regulated via induced retinoid signaling. Experiment Overall Design: Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF, cytokine treatment was repeated at day 3. Ligands were added at the beginning of differentiation but AGN193109 (AGN) treatment was repeated at day 3. Cells were obtained from three healthy individuals (three biological replicates) and cells were treated with vehicle (DC), 2.5 uM rosiglitazone (DC RSG), 100 nM AM580 (DC AM). RSG treatment was also combined with 1 uM AGN193109 (DC RSG AGN) in this case two biological replicates was used (RNA was obtained from 2. and 3. individuals).
AC011899.9 UP in 'rosiglitazone' vs 'none'
FILIP1L DOWN in 'rosiglitazone' vs 'none'
MARCKSL1 DOWN in 'rosiglitazone' vs 'none'
MARCKS DOWN in 'rosiglitazone' vs 'none'
TUBB2B DOWN in 'rosiglitazone' vs 'none'
CTTNBP2 DOWN in 'rosiglitazone' vs 'none'
SELPLG DOWN in 'rosiglitazone' vs 'none'
IL1RAP DOWN in 'rosiglitazone' vs 'none'
PRKAR2B DOWN in 'rosiglitazone' vs 'none'
HEXIM1 DOWN in 'rosiglitazone' vs 'none'
SLC7A11 DOWN in 'rosiglitazone' vs 'none'
SLC16A6 DOWN in 'rosiglitazone' vs 'none'
PTGER4 DOWN in 'rosiglitazone' vs 'none'
MAN1C1 DOWN in 'rosiglitazone' vs 'none'
KIAA1671 DOWN in 'rosiglitazone' vs 'none'
PHLDA2 DOWN in 'rosiglitazone' vs 'none'
ARHGEF28 UP in 'rosiglitazone' vs 'none'
TNFRSF21 UP in 'rosiglitazone' vs 'none'
GOLGA8A DOWN in 'rosiglitazone' vs 'none'
RAP1GAP DOWN in 'rosiglitazone' vs 'none'
PSTPIP2 DOWN in 'rosiglitazone' vs 'none'
MAP3K13 DOWN in 'rosiglitazone' vs 'none'
AP001053.11 UP in 'rosiglitazone' vs 'none'
ISYNA1 DOWN in 'rosiglitazone' vs 'none'
WFDC21P DOWN in 'rosiglitazone' vs 'none'
SLC30A4 DOWN in 'rosiglitazone' vs 'none'
TMEM194A DOWN in 'rosiglitazone' vs 'none'
SIGLEC17P UP in 'rosiglitazone' vs 'none'
LIMCH1 DOWN in 'rosiglitazone' vs 'none'
RP11-38P22.2 UP in 'rosiglitazone' vs 'none'
TACSTD2 DOWN in 'rosiglitazone' vs 'none'
TMEM45B DOWN in 'rosiglitazone' vs 'none'
TSPAN33 DOWN in 'rosiglitazone' vs 'none'
AGPAT9 DOWN in 'rosiglitazone' vs 'none'
PXYLP1 DOWN in 'rosiglitazone' vs 'none'
TMEM97 DOWN in 'rosiglitazone' vs 'none'
(Factor value) COMPOUND/rosiglitazone and AGN193109
SLC39A10 DOWN in 'rosiglitazone' vs 'none'
C4orf32 DOWN in 'rosiglitazone' vs 'none'
CLEC10A DOWN in 'rosiglitazone' vs 'none'
CCDC109B UP in 'rosiglitazone' vs 'none'
CEP112 DOWN in 'rosiglitazone' vs 'none'
DOCK10 DOWN in 'rosiglitazone' vs 'none'
SH3BP4 DOWN in 'rosiglitazone' vs 'none'
SLCO5A1 DOWN in 'rosiglitazone' vs 'none'
GABARAPL1 UP in 'rosiglitazone' vs 'none'
ADAM19 DOWN in 'rosiglitazone' vs 'none'
IPCEF1 DOWN in 'rosiglitazone' vs 'none'