UGT85A1 UP in 'pho1 mutant' vs 'wild type'
MI-1-P SYNTHASE DOWN in 'pho1 mutant' vs 'wild type'
WRKY25 UP in 'pho1 mutant' vs 'wild type'
CYP81D8 UP in 'pho1 mutant' vs 'wild type'
AT1G22170 UP in 'pho1 mutant' vs 'wild type'
AtRLP23 UP in 'pho1 mutant' vs 'wild type'
AT3G07350 UP in 'pho1 mutant' vs 'wild type'
AT1G13470 UP in 'pho1 mutant' vs 'wild type'
CER4 DOWN in 'pho1 mutant' vs 'wild type'
AT4G19880 UP in 'pho1 mutant' vs 'wild type'
AT3G04350 UP in 'pho1 mutant' vs 'wild type'
AtPPa5 UP in 'pho1 mutant' vs 'wild type'
AT3G59300 UP in 'pho1 mutant' vs 'wild type'
CIPK9 UP in 'pho1 mutant' vs 'wild type'
Background: The UK horticultural and agricultural industries routinely apply large amounts of inorganic fertiliser to maintain crop yield and quality, since chemical assays of soil nutrients are unreliable. Excessive fertiliser applications are costly and can lead to unnecessary pollution. A possible solution is to use sensor (GM or non-GM) technologies that exploit the changes in plant gene expression under incipient nutrient deficiency. Aim: The aim of this project is to use mutants with reduced leaf phosphate contents to identify genes upregulated in response to phosphate stress. Preliminary gene expression analysis has identified several phosphate responsive genes to be upregulated in the pho1 mutant. However, further replicates of the experiment are required to confirm these changes. Methods: Arabidopsis mutant pho1 (N8507) and its parent ecotype Columbia 2 (N907) will be grown on MS agar under identical conditions. RNA will be extracted from the rosette leaves of both parent and mutant and the same growth stage. By comparing the expression profiles, we will be able to differentiate between genes that are upregulated in leaves experiencing phosphate stress. Previously, two GeneChips have been used (mutant and parent) to provide preliminary data. A further two biological replicates are now required to confirm these results. Promoters and transcripts of these genes will underpin the development of novel sensor technologies, and knowledge of the gene expression profiles will improve our understanding of the physiology of plant mineral nutrition. Experimenter name = John Hammond; Experimenter phone = 01789 470382; Experimenter fax = 01789 470552; Experimenter address = Plant Sciences Division; Experimenter address = School of Biosciences; Experimenter address = University of Nottingham; Experimenter address = Sutton Bonington Campus; Experimenter address = Loughborough; Experimenter zip/postal_code = LE12 5RD; Experimenter country = UK Experiment Overall Design: 6 samples were used in this experiment
AT1G74440 UP in 'pho1 mutant' vs 'wild type'
AT3G16660 UP in 'pho1 mutant' vs 'wild type'
ATPAP14 UP in 'pho1 mutant' vs 'wild type'
ATDGK3 UP in 'pho1 mutant' vs 'wild type'
Analysis of 'pho1 mutant' vs 'wild type'
AT2G24160 UP in 'pho1 mutant' vs 'wild type'
AT2G18690 UP in 'pho1 mutant' vs 'wild type'
PLDP2 UP in 'pho1 mutant' vs 'wild type'
ATMRP7 UP in 'pho1 mutant' vs 'wild type'
AT4G28040 UP in 'pho1 mutant' vs 'wild type'
CNGC12 UP in 'pho1 mutant' vs 'wild type'
AT2G30550 UP in 'pho1 mutant' vs 'wild type'
AT4G31240 UP in 'pho1 mutant' vs 'wild type'
PME17 UP in 'pho1 mutant' vs 'wild type'
T2E22.34 UP in 'pho1 mutant' vs 'wild type'
AT1G07440 DOWN in 'pho1 mutant' vs 'wild type'
CYP81F2 UP in 'pho1 mutant' vs 'wild type'
(Assay) Hammond_2-2_pho1mutant_Rep1_ATH1
AT4G23000 UP in 'pho1 mutant' vs 'wild type'
ATMRP14 UP in 'pho1 mutant' vs 'wild type'
AT3G19850 DOWN in 'pho1 mutant' vs 'wild type'
ATPAP22 UP in 'pho1 mutant' vs 'wild type'
GRXS1 UP in 'pho1 mutant' vs 'wild type'
CDKC2 UP in 'pho1 mutant' vs 'wild type'
AT3G05730 UP in 'pho1 mutant' vs 'wild type'
NF-YA7 UP in 'pho1 mutant' vs 'wild type'
ATACP5 UP in 'pho1 mutant' vs 'wild type'
CYP71B23 UP in 'pho1 mutant' vs 'wild type'
UGT84A2 UP in 'pho1 mutant' vs 'wild type'
AT1G68670 UP in 'pho1 mutant' vs 'wild type'
CDKB2;2 UP in 'pho1 mutant' vs 'wild type'
AT3G22850 UP in 'pho1 mutant' vs 'wild type'
CRK14 UP in 'pho1 mutant' vs 'wild type'
AT3G60420 UP in 'pho1 mutant' vs 'wild type'
ATSR1 UP in 'pho1 mutant' vs 'wild type'
AT1G76520 UP in 'pho1 mutant' vs 'wild type'
AT2G38740 UP in 'pho1 mutant' vs 'wild type'
WRKY45 UP in 'pho1 mutant' vs 'wild type'
GRXS4 UP in 'pho1 mutant' vs 'wild type'
ATPLT5 UP in 'pho1 mutant' vs 'wild type'
AT3G27150 UP in 'pho1 mutant' vs 'wild type'
WRKY46 UP in 'pho1 mutant' vs 'wild type'
RSW10 UP in 'pho1 mutant' vs 'wild type'
PAP10 UP in 'pho1 mutant' vs 'wild type'
AT1G26390 UP in 'pho1 mutant' vs 'wild type'
TIFY10A UP in 'pho1 mutant' vs 'wild type'
BGLU11 UP in 'pho1 mutant' vs 'wild type'
AT1G13340 UP in 'pho1 mutant' vs 'wild type'
PDF2.3 UP in 'pho1 mutant' vs 'wild type'
MERI5B UP in 'pho1 mutant' vs 'wild type'
CML47 UP in 'pho1 mutant' vs 'wild type'
AT1G57630 UP in 'pho1 mutant' vs 'wild type'
(Assay) Hammond_2-4_pho1mutant_Rep2_ATH1
HIPP22 UP in 'pho1 mutant' vs 'wild type'
AT2G22080 UP in 'pho1 mutant' vs 'wild type'
AtOCT4 UP in 'pho1 mutant' vs 'wild type'
AT4G03960 UP in 'pho1 mutant' vs 'wild type'
NHL12 DOWN in 'pho1 mutant' vs 'wild type'
AT3G22600 UP in 'pho1 mutant' vs 'wild type'
AT1G08310 UP in 'pho1 mutant' vs 'wild type'
AT4G38540 UP in 'pho1 mutant' vs 'wild type'
AT1G67600 UP in 'pho1 mutant' vs 'wild type'
AT1G72070 UP in 'pho1 mutant' vs 'wild type'
BMY5 DOWN in 'pho1 mutant' vs 'wild type'
AT4G20860 UP in 'pho1 mutant' vs 'wild type'
WRKY70 UP in 'pho1 mutant' vs 'wild type'
AT1G17710 UP in 'pho1 mutant' vs 'wild type'
AT4G32870 UP in 'pho1 mutant' vs 'wild type'
AT3G04000 UP in 'pho1 mutant' vs 'wild type'
AT3G54150 UP in 'pho1 mutant' vs 'wild type'
WRKY54 UP in 'pho1 mutant' vs 'wild type'
AT1G74360 UP in 'pho1 mutant' vs 'wild type'
ATHCHIB UP in 'pho1 mutant' vs 'wild type'
AT4G11890 UP in 'pho1 mutant' vs 'wild type'
PUB24 UP in 'pho1 mutant' vs 'wild type'
AT2G46220 UP in 'pho1 mutant' vs 'wild type'
AT3G14700 UP in 'pho1 mutant' vs 'wild type'
AT3G48080 UP in 'pho1 mutant' vs 'wild type'
SHA1 DOWN in 'pho1 mutant' vs 'wild type'
CML41 UP in 'pho1 mutant' vs 'wild type'
AT1G73805 UP in 'pho1 mutant' vs 'wild type'
XTR8 DOWN in 'pho1 mutant' vs 'wild type'
AtGolS1 UP in 'pho1 mutant' vs 'wild type'
GRXS9 UP in 'pho1 mutant' vs 'wild type'
ATSPX3 UP in 'pho1 mutant' vs 'wild type'
ATBCB UP in 'pho1 mutant' vs 'wild type'
AT5G67600 UP in 'pho1 mutant' vs 'wild type'
ATJRG21 UP in 'pho1 mutant' vs 'wild type'
AT1G67920 UP in 'pho1 mutant' vs 'wild type'
COR414-TM1 DOWN in 'pho1 mutant' vs 'wild type'
AT2G24600 UP in 'pho1 mutant' vs 'wild type'
ATPAP23 UP in 'pho1 mutant' vs 'wild type'
GRX480 UP in 'pho1 mutant' vs 'wild type'
ATEXPA11 DOWN in 'pho1 mutant' vs 'wild type'
LSF2 DOWN in 'pho1 mutant' vs 'wild type'
PHT3;2 UP in 'pho1 mutant' vs 'wild type'
AT3G11230 UP in 'pho1 mutant' vs 'wild type'
AT1G53035 DOWN in 'pho1 mutant' vs 'wild type'
AT3G13910 UP in 'pho1 mutant' vs 'wild type'
AT1G23140 UP in 'pho1 mutant' vs 'wild type'
CRRSP38 UP in 'pho1 mutant' vs 'wild type'
AT3G19970 UP in 'pho1 mutant' vs 'wild type'
ATTRX5 UP in 'pho1 mutant' vs 'wild type'
AT3G23870 UP in 'pho1 mutant' vs 'wild type'
ATVAMP713 DOWN in 'pho1 mutant' vs 'wild type'
AT5G02760 DOWN in 'pho1 mutant' vs 'wild type'
AT4G34950 UP in 'pho1 mutant' vs 'wild type'
AT5G03350 UP in 'pho1 mutant' vs 'wild type'
AT1G35710 UP in 'pho1 mutant' vs 'wild type'
ATEXPB3 DOWN in 'pho1 mutant' vs 'wild type'
ATAAH DOWN in 'pho1 mutant' vs 'wild type'
AT5G63130 UP in 'pho1 mutant' vs 'wild type'
ATMES9 UP in 'pho1 mutant' vs 'wild type'
CRK37 UP in 'pho1 mutant' vs 'wild type'
GSTL1 UP in 'pho1 mutant' vs 'wild type'
AT4G27280 UP in 'pho1 mutant' vs 'wild type'
AT5G41050 DOWN in 'pho1 mutant' vs 'wild type'
AT1G29500 DOWN in 'pho1 mutant' vs 'wild type'
ATPAP25 UP in 'pho1 mutant' vs 'wild type'
AT1G19020 UP in 'pho1 mutant' vs 'wild type'
PNP-A UP in 'pho1 mutant' vs 'wild type'
ATEXO70B2 UP in 'pho1 mutant' vs 'wild type'
FKBP16-2 DOWN in 'pho1 mutant' vs 'wild type'
AT5G63680 UP in 'pho1 mutant' vs 'wild type'
COR314-TM2 DOWN in 'pho1 mutant' vs 'wild type'
ATPS2 UP in 'pho1 mutant' vs 'wild type'
FMO GS-OX2 UP in 'pho1 mutant' vs 'wild type'
ALIS1 UP in 'pho1 mutant' vs 'wild type'
HSPRO2 UP in 'pho1 mutant' vs 'wild type'
AT2G21830 DOWN in 'pho1 mutant' vs 'wild type'
UGT86A1 UP in 'pho1 mutant' vs 'wild type'
AT1G21520 UP in 'pho1 mutant' vs 'wild type'
AT4G12490 UP in 'pho1 mutant' vs 'wild type'
AIG2L UP in 'pho1 mutant' vs 'wild type'
AT2G41250 UP in 'pho1 mutant' vs 'wild type'
KUP11 UP in 'pho1 mutant' vs 'wild type'
ATPAP16 UP in 'pho1 mutant' vs 'wild type'
AT1G66880 UP in 'pho1 mutant' vs 'wild type'
AT5G25440 UP in 'pho1 mutant' vs 'wild type'
ATSPX2 UP in 'pho1 mutant' vs 'wild type'
ATFMN/FHY UP in 'pho1 mutant' vs 'wild type'
AT2G20670 UP in 'pho1 mutant' vs 'wild type'
GH3.6 UP in 'pho1 mutant' vs 'wild type'
AT1G72900 UP in 'pho1 mutant' vs 'wild type'
IP5PII UP in 'pho1 mutant' vs 'wild type'
AT4G03450 UP in 'pho1 mutant' vs 'wild type'
AT3G22750 UP in 'pho1 mutant' vs 'wild type'
WRKY40 UP in 'pho1 mutant' vs 'wild type'
AT1G52800 UP in 'pho1 mutant' vs 'wild type'
BAS1 DOWN in 'pho1 mutant' vs 'wild type'
AT2G39210 UP in 'pho1 mutant' vs 'wild type'
WSD1 DOWN in 'pho1 mutant' vs 'wild type'
AGL87 DOWN in 'pho1 mutant' vs 'wild type'
AT1G24575 UP in 'pho1 mutant' vs 'wild type'
AT4G17790 UP in 'pho1 mutant' vs 'wild type'
ERF070 UP in 'pho1 mutant' vs 'wild type'
AT3G02910 DOWN in 'pho1 mutant' vs 'wild type'
AT1G24140 UP in 'pho1 mutant' vs 'wild type'
ACA.l UP in 'pho1 mutant' vs 'wild type'
AtRLP19 UP in 'pho1 mutant' vs 'wild type'
AT4G02360 UP in 'pho1 mutant' vs 'wild type'
BETA-VPE UP in 'pho1 mutant' vs 'wild type'
CAF1-9 UP in 'pho1 mutant' vs 'wild type'
AT5G24210 UP in 'pho1 mutant' vs 'wild type'
AT2G16660 UP in 'pho1 mutant' vs 'wild type'
AT4G00500 UP in 'pho1 mutant' vs 'wild type'
AT3G18250 UP in 'pho1 mutant' vs 'wild type'
AT1G05575 UP in 'pho1 mutant' vs 'wild type'
AT3G12830 UP in 'pho1 mutant' vs 'wild type'
AT4G12830 DOWN in 'pho1 mutant' vs 'wild type'
AT4G25150 UP in 'pho1 mutant' vs 'wild type'
AT1G30250 UP in 'pho1 mutant' vs 'wild type'
KUP10 UP in 'pho1 mutant' vs 'wild type'
SCPL51 DOWN in 'pho1 mutant' vs 'wild type'
AT2G34210 UP in 'pho1 mutant' vs 'wild type'
AT1G22410 UP in 'pho1 mutant' vs 'wild type'
AT3G19010 UP in 'pho1 mutant' vs 'wild type'
NUDT7 UP in 'pho1 mutant' vs 'wild type'
SYP122 UP in 'pho1 mutant' vs 'wild type'
AtRLP41 UP in 'pho1 mutant' vs 'wild type'
ATMES18 DOWN in 'pho1 mutant' vs 'wild type'
AT1G19200 UP in 'pho1 mutant' vs 'wild type'
AT1G23120 UP in 'pho1 mutant' vs 'wild type'
AT3G61410 UP in 'pho1 mutant' vs 'wild type'
AT5G19240 UP in 'pho1 mutant' vs 'wild type'
CER1 DOWN in 'pho1 mutant' vs 'wild type'
AT4G17470 UP in 'pho1 mutant' vs 'wild type'
PSK1 DOWN in 'pho1 mutant' vs 'wild type'
AT3G19660 UP in 'pho1 mutant' vs 'wild type'
AT1G22160 DOWN in 'pho1 mutant' vs 'wild type'
ATPAP24 UP in 'pho1 mutant' vs 'wild type'
AtOCT1 UP in 'pho1 mutant' vs 'wild type'
ATGSTU11 UP in 'pho1 mutant' vs 'wild type'
AT3G21620 UP in 'pho1 mutant' vs 'wild type'
ATERF-1 UP in 'pho1 mutant' vs 'wild type'
AT1G72890 UP in 'pho1 mutant' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
ATGSTU10 UP in 'pho1 mutant' vs 'wild type'
AT3G09050 DOWN in 'pho1 mutant' vs 'wild type'
EIF-5A DOWN in 'pho1 mutant' vs 'wild type'
AT4G35750 UP in 'pho1 mutant' vs 'wild type'
AT1G34640 UP in 'pho1 mutant' vs 'wild type'
AT4G09350 DOWN in 'pho1 mutant' vs 'wild type'
AT3G14067 DOWN in 'pho1 mutant' vs 'wild type'
AT2G15042 UP in 'pho1 mutant' vs 'wild type'
ATNUDT6 UP in 'pho1 mutant' vs 'wild type'
anac036 UP in 'pho1 mutant' vs 'wild type'
AT3G43110 UP in 'pho1 mutant' vs 'wild type'
AT2G25735 UP in 'pho1 mutant' vs 'wild type'
ITPK3 UP in 'pho1 mutant' vs 'wild type'
AT4G19810 UP in 'pho1 mutant' vs 'wild type'
AT3G04640 UP in 'pho1 mutant' vs 'wild type'
GRXS13 UP in 'pho1 mutant' vs 'wild type'
ATPAH1 UP in 'pho1 mutant' vs 'wild type'
AT4G01700 UP in 'pho1 mutant' vs 'wild type'
ATTI4 UP in 'pho1 mutant' vs 'wild type'
AT1G74210 UP in 'pho1 mutant' vs 'wild type'
ATMPK3 UP in 'pho1 mutant' vs 'wild type'
WRKY38 UP in 'pho1 mutant' vs 'wild type'
AT2G17280 UP in 'pho1 mutant' vs 'wild type'
(Assay) Hammond_2-6_pho1mutant_Rep3_ATH1
AT4G12500 UP in 'pho1 mutant' vs 'wild type'
PME12 UP in 'pho1 mutant' vs 'wild type'
AT2G45210 UP in 'pho1 mutant' vs 'wild type'
PHT1;9 UP in 'pho1 mutant' vs 'wild type'
PAP3 DOWN in 'pho1 mutant' vs 'wild type'
AT1G78020 DOWN in 'pho1 mutant' vs 'wild type'
ATPPC1 UP in 'pho1 mutant' vs 'wild type'
AT3G04210 UP in 'pho1 mutant' vs 'wild type'
CRK36 UP in 'pho1 mutant' vs 'wild type'
EDA39 UP in 'pho1 mutant' vs 'wild type'
ATCWINV1 UP in 'pho1 mutant' vs 'wild type'
ATPK19 UP in 'pho1 mutant' vs 'wild type'
(Assay) Hammond_2-3_Col2wildtype_Rep2_ATH1
PPCK2 UP in 'pho1 mutant' vs 'wild type'
XBAT34 UP in 'pho1 mutant' vs 'wild type'
CYP83B1 UP in 'pho1 mutant' vs 'wild type'
AT1G17830 UP in 'pho1 mutant' vs 'wild type'
ATCHX17 UP in 'pho1 mutant' vs 'wild type'
SOBIR1 UP in 'pho1 mutant' vs 'wild type'
AT2G23680 UP in 'pho1 mutant' vs 'wild type'
ATPS3 UP in 'pho1 mutant' vs 'wild type'
AT1G72060 UP in 'pho1 mutant' vs 'wild type'
COPT2 DOWN in 'pho1 mutant' vs 'wild type'
AT2G15020 DOWN in 'pho1 mutant' vs 'wild type'
ATEXT3 UP in 'pho1 mutant' vs 'wild type'
(Assay) Hammond_2-5_Col2wildtype_Rep3_ATH1
AT4G00700 UP in 'pho1 mutant' vs 'wild type'
AT3G44510 UP in 'pho1 mutant' vs 'wild type'
AT1G05000 UP in 'pho1 mutant' vs 'wild type'
AT4G28260 UP in 'pho1 mutant' vs 'wild type'
AT1G70900 UP in 'pho1 mutant' vs 'wild type'
SYP121 UP in 'pho1 mutant' vs 'wild type'
ATMPK11 UP in 'pho1 mutant' vs 'wild type'
AT1G53620 UP in 'pho1 mutant' vs 'wild type'
AT3G51660 UP in 'pho1 mutant' vs 'wild type'
AT4G38550 UP in 'pho1 mutant' vs 'wild type'
EDS16 UP in 'pho1 mutant' vs 'wild type'
ATLCAT3 UP in 'pho1 mutant' vs 'wild type'
AT5G17340 UP in 'pho1 mutant' vs 'wild type'
AT1G13750 UP in 'pho1 mutant' vs 'wild type'
AT4G26620 UP in 'pho1 mutant' vs 'wild type'
BEE2 DOWN in 'pho1 mutant' vs 'wild type'
AtPPa4 UP in 'pho1 mutant' vs 'wild type'
ALA10 UP in 'pho1 mutant' vs 'wild type'
AT1G15040 UP in 'pho1 mutant' vs 'wild type'
ATMYB48-1 DOWN in 'pho1 mutant' vs 'wild type'
CML40 UP in 'pho1 mutant' vs 'wild type'
AT3G48650 UP in 'pho1 mutant' vs 'wild type'
SDR3A UP in 'pho1 mutant' vs 'wild type'
AT1G17615 UP in 'pho1 mutant' vs 'wild type'
NIMIN1 UP in 'pho1 mutant' vs 'wild type'
PHO1;H1 UP in 'pho1 mutant' vs 'wild type'
(Sample) developmental stage/Boyes key 3.90
AT3G03790 UP in 'pho1 mutant' vs 'wild type'
AT5G12050 DOWN in 'pho1 mutant' vs 'wild type'
ATPSK4 UP in 'pho1 mutant' vs 'wild type'
NIR1 DOWN in 'pho1 mutant' vs 'wild type'
AT1G25550 UP in 'pho1 mutant' vs 'wild type'
CIPK12 UP in 'pho1 mutant' vs 'wild type'
WRKY18 UP in 'pho1 mutant' vs 'wild type'
WRKY33 UP in 'pho1 mutant' vs 'wild type'
BGLU44 UP in 'pho1 mutant' vs 'wild type'
PPCK1 UP in 'pho1 mutant' vs 'wild type'
AT2G37890 UP in 'pho1 mutant' vs 'wild type'
AT3G51440 UP in 'pho1 mutant' vs 'wild type'
(Assay) Hammond_2-1_Col2wildtype_Rep1_ATH1
AT-HSFA4A UP in 'pho1 mutant' vs 'wild type'
AT1G10340 UP in 'pho1 mutant' vs 'wild type'
ANNAT1 UP in 'pho1 mutant' vs 'wild type'
AT1G52870 DOWN in 'pho1 mutant' vs 'wild type'
AT1G78410 UP in 'pho1 mutant' vs 'wild type'
AT3G45160 DOWN in 'pho1 mutant' vs 'wild type'
AT5G13190 UP in 'pho1 mutant' vs 'wild type'
AT3G26510 UP in 'pho1 mutant' vs 'wild type'
SAG21 UP in 'pho1 mutant' vs 'wild type'