(Assay) PGA-Pi3k_dn_1451v2
type: Assay
has participant: A-AFFY-6

(Assay) PGA-Pi3k_ntg_3119v2
type: Assay
has participant: A-AFFY-6

Cpxm2
type: EnsemblDatabaseReference

E-GEOD-558
type: microarray 1colour mrna differential
RO_0002162: Mus musculus
description: The insulin/IGF-IRS-PI3K-Akt pathway is highly conserved in evolution. To test whether this signaling cascade determines organ size in vertebrates, we have recently created mouse lines transgenic for constitutively active (caPI3K) and dominant-negative forms of PI3K (dnPI3K) (Shioi et al., 2000). Transgene expression is driven by the cardiac-specific a -myosin heavy chain (MHC) promoter. Both transgenic lines are characterized by moderate but highly consistent changes in heart size, despite the fact that body weight and the size of other organs are completely normal. caPI3K transgenic animals have hearts 20% larger than those of wild type littermates, whereas dnPI3K mice have hearts that are 17% smaller. The observed changes in heart size correlate with an appropriate increase or decrease in the size of transgenic cardiomyocytes. These hearts do not have cardiomyopathic changes, since the contractile function of transgenic hearts is normal, and signs of necrosis, apoptosis, or interstitial fibrosis are absent (see Physiology). These data suggest an autonomous role for PI3K in cell size regulation. Our goal is now to determine how activation of this system translates into changes in cell size by identifying the trigger and components of this signaling cascade. Heterozygous caPI3K (constitutively active PI3K) and dnPI3K (dominant-negative PI3K) transgenic female mice (5-14 months) compared with non-transgenic littermates. Transgene expression driven by the a -myosin heavy chain (a -MHC) promoter.
uses data from: pubmed

Rab10
type: EnsemblDatabaseReference

(Sample) phenotype/normal
type: EFO_0000761
property type: phenotype
property value: normal

Nppa UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.58814366666667
p value: 0.0241113052553411
refers to: Nppa
refers to: 103593_at
t statistic: 3.66568692760012

102227_g_at
type: EFO_0005072
part of: A-AFFY-6

98616_f_at
type: EFO_0005072
part of: A-AFFY-6

160614_at
type: EFO_0005072
part of: A-AFFY-6

Rbm39 DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.17340023333333
p value: 1.00468520761795E-5
refers to: 94689_at
refers to: Rbm39
t statistic: -19.0086485101434

Tpm2
type: EnsemblDatabaseReference

(Sample) organism/Mus musculus
type: Mus musculus
property type: organism
property value: Mus musculus

Pah UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.19999376666667
p value: 1.00468520761795E-5
refers to: 95407_at
refers to: Pah
t statistic: 19.6892238195836

Ppara
type: EnsemblDatabaseReference

Ctgf
type: EnsemblDatabaseReference

Tpm2
type: EnsemblDatabaseReference

Yam1
type: EnsemblDatabaseReference

Myl7
type: EnsemblDatabaseReference

Pah
type: EnsemblDatabaseReference

Pik3r1 DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.8660565
p value: 1.80876118161514E-5
refers to: Pik3r1
refers to: 96592_at
t statistic: -15.4489163823299

Myh7 UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.061164
p value: 3.62214898743261E-4
refers to: Myh7
refers to: 98616_f_at
t statistic: 9.52974888609752

95407_at
type: EFO_0005072
part of: A-AFFY-6

(Factor value) GENOTYPE/wild type genotype
type: EFO_0000001
property type: GENOTYPE
property value: wild type genotype

Phlda1 UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.3024898
p value: 9.47749428685317E-5
refers to: 160829_at
refers to: Phlda1
t statistic: 11.9372880864412

Ctgf UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.38660483333333
p value: 1.66830872807092E-4
refers to: 93294_at
refers to: Ctgf
t statistic: 10.7880340745679

Bex1 UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.5047287
p value: 1.05304781005115E-5
refers to: Bex1
refers to: 93020_at
t statistic: 17.8831014680798

94192_at
type: EFO_0005072
part of: A-AFFY-6

Bex1
type: EnsemblDatabaseReference

(Assay) PGA-Pi3k_ca_2817v2
type: Assay
has participant: A-AFFY-6

96592_at
type: EFO_0005072
part of: A-AFFY-6

101933_at
type: EFO_0005072
part of: A-AFFY-6

Myl1 DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.6197905
p value: 2.323809992491E-5
refers to: Myl1
refers to: 92541_at
t statistic: -14.6257434028481

Ppara DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.28504603333333
p value: 1.65216568581257E-5
refers to: Ppara
refers to: 102668_at
t statistic: -15.9513711259299

Dynll2
type: EnsemblDatabaseReference

Wnk1 DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.740659
p value: 3.72412818515127E-5
refers to: 160739_at
refers to: Wnk1
t statistic: -13.7707159617459

Analysis of 'dominant-negative caPI3K transgenic' vs 'wild type'
type: MicroarrayDifferentialExpressionAnalysis

Casq1
type: EnsemblDatabaseReference

Cpxm2
type: EnsemblDatabaseReference

100605_at
type: EFO_0005072
part of: A-AFFY-6

Phlda1
type: EnsemblDatabaseReference

Myh7
type: EnsemblDatabaseReference

Pten DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.0373844
p value: 1.65216568581257E-5
refers to: Pten
refers to: 160614_at
t statistic: -16.1156519527271

(Sample) organism part/ventricle
type: UBERON_0002082
property type: organism part
property value: ventricle

Mus musculus
type: OBI_0100026

100403_at
type: EFO_0005072
part of: A-AFFY-6

Gdap10
type: EnsemblDatabaseReference

(Sample) genotype/constitutively active caPI3K transgenic
type: EFO_0000001
property type: genotype
property value: constitutively active caPI3K transgenic

A-AFFY-6
type: EFO_0000269

Cpxm2 UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.30437856666667
p value: 2.323809992491E-5
refers to: 102227_g_at
refers to: Cpxm2
t statistic: 14.6754665718758

Yam1 UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 2.32193133333334
p value: 4.94295150441918E-4
refers to: Yam1
refers to: AFFX-18SRNAMur/X00686_3_at
t statistic: 8.76010283975501

Acta1
type: EnsemblDatabaseReference

Acta1 UP in 'constitutively active caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.25042516666667
p value: 0.0053635924540898
refers to: Acta1
refers to: 100381_at
t statistic: 10.7019296378933

Dynll2 UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.16742283333333
p value: 0.00255316848708981
refers to: Dynll2
refers to: 101929_at
t statistic: 6.515673903373

(Sample) genotype/dominant-negative caPI3K transgenic
type: EFO_0000001
property type: genotype
property value: dominant-negative caPI3K transgenic

Myl1
type: EnsemblDatabaseReference

(Assay) PGA-Pi3k_dn_1294v2
type: Assay
has participant: A-AFFY-6

(Factor value) GENOTYPE/constitutively active caPI3K transgenic
type: EFO_0000001
property type: GENOTYPE
property value: constitutively active caPI3K transgenic

Rab10 UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.0414547
p value: 4.39030116719849E-4
refers to: Rab10
refers to: 101933_at
t statistic: 8.9521262552077

Pah DOWN in 'constitutively active caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.17156163333333
p value: 0.0124682101906311
refers to: Pah
refers to: 95407_at
t statistic: -6.75921762005152

101929_at
type: EFO_0005072
part of: A-AFFY-6

Wnk1
type: EnsemblDatabaseReference

Pik3r1
type: EnsemblDatabaseReference

(Factor value) GENOTYPE/dominant-negative caPI3K transgenic
type: EFO_0000001
property type: GENOTYPE
property value: dominant-negative caPI3K transgenic

100381_at
type: EFO_0005072
part of: A-AFFY-6

Pik3r1
type: EnsemblDatabaseReference

Myl4
type: EnsemblDatabaseReference

(Sample) phenotype/heart hypotrophy
type: EFO_0000001
property type: phenotype
property value: heart hypotrophy

103593_at
type: EFO_0005072
part of: A-AFFY-6

93294_at
type: EFO_0005072
part of: A-AFFY-6

Nppa
type: EnsemblDatabaseReference

Casq1
type: EnsemblDatabaseReference

160487_at
type: EFO_0005072
part of: A-AFFY-6

Psap
type: EnsemblDatabaseReference

160739_at
type: EFO_0005072
part of: A-AFFY-6

Phlda1
type: EnsemblDatabaseReference

Myh7
type: EnsemblDatabaseReference

Rbm39
type: EnsemblDatabaseReference

Nppa
type: EnsemblDatabaseReference

Pten
type: EnsemblDatabaseReference

(Assay) PGA-Pi3k_dn_1449v2
type: Assay
has participant: A-AFFY-6

Analysis of 'constitutively active caPI3K transgenic' vs 'wild type'
type: MicroarrayDifferentialExpressionAnalysis

(Assay) PGA-Pi3k_ca_3120v2
type: Assay
has participant: A-AFFY-6

Gdap10
type: EnsemblDatabaseReference

Rbm39
type: EnsemblDatabaseReference

Acta1
type: EnsemblDatabaseReference

Gdap10 DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.7880949
p value: 4.98598867711949E-6
refers to: Gdap10
refers to: 94192_at
t statistic: -23.1292451383253

Pten
type: EnsemblDatabaseReference

Rab10
type: EnsemblDatabaseReference

Psap UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.0574036
p value: 8.676058840655E-5
refers to: Psap
refers to: 97560_at
t statistic: 12.1240261574266

Myl4 DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -3.57269783333333
p value: 0.0150157094624482
refers to: Myl4
refers to: 160487_at
t statistic: -4.17928832607563

Myl1
type: EnsemblDatabaseReference

Tpm2 UP in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.10447616666667
p value: 1.65216568581257E-5
refers to: Tpm2
refers to: 100605_at
t statistic: 15.9872354236471

160829_at
type: EFO_0005072
part of: A-AFFY-6

92541_at
type: EFO_0005072
part of: A-AFFY-6

Ppara
type: EnsemblDatabaseReference

Wnk1
type: EnsemblDatabaseReference

97560_at
type: EFO_0005072
part of: A-AFFY-6

(Assay) PGA-Pi3k_ntg_2820v2
type: Assay
has participant: A-AFFY-6

Ctgf
type: EnsemblDatabaseReference

Yam1
type: EnsemblDatabaseReference

93020_at
type: EFO_0005072
part of: A-AFFY-6

(Sample) genotype/wild type genotype
type: EFO_0000001
property type: genotype
property value: wild type genotype

Hsph1
type: EnsemblDatabaseReference

98111_at
type: EFO_0005072
part of: A-AFFY-6

Myl7
type: EnsemblDatabaseReference

Pah
type: EnsemblDatabaseReference

Myl4
type: EnsemblDatabaseReference

AFFX-18SRNAMur/X00686_3_at
type: EFO_0005072
part of: A-AFFY-6

Hsph1
type: EnsemblDatabaseReference

Hsph1 UP in 'constitutively active caPI3K transgenic' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.03949196666667
p value: 0.006408087020838
refers to: Hsph1
refers to: 98111_at
t statistic: 9.59108732597905

94689_at
type: EFO_0005072
part of: A-AFFY-6

102426_at
type: EFO_0005072
part of: A-AFFY-6

102668_at
type: EFO_0005072
part of: A-AFFY-6

(Assay) PGA-Pi3k_ntg_2819v2
type: Assay
has participant: A-AFFY-6

Bex1
type: EnsemblDatabaseReference

(Sample) phenotype/heart hypertrophy
type: EFO_0002503
property type: phenotype
property value: heart hypertrophy

Psap
type: EnsemblDatabaseReference

(Assay) PGA-Pi3k_ca_2818v2
type: Assay
has participant: A-AFFY-6

Myl7 DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -3.4069929
p value: 0.0134312799084362
refers to: 100403_at
refers to: Myl7
t statistic: -4.31076952976518

Casq1 DOWN in 'dominant-negative caPI3K transgenic' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.29955213333333
p value: 2.31778465434929E-4
refers to: Casq1
refers to: 102426_at
t statistic: -10.2703879245646

Dynll2
type: EnsemblDatabaseReference