w UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
TpnC4 DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
Analysis of 'HEXIM knockdown mutant' vs 'wild type genotype'
Hsp23 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
Met DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
Cpr92F DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
CG33468 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
We address the function of HEXIM, an inhibitor of the general transcriptional elongation regulator P-TEFb which regulates the transcriptional status of many developmental genes, during Drosophila development. We showed that HEXIM knockdown mutants display organs development failure. In the wing disc, it induces apoptosis and affects Hh signaling. The continuous death of proliferative cells is compensated by apoptosis-induced cell proliferation, in a manner similar to that of differentiated cells, together with high levels of Hh and Ci. We completed this analysis with microarrays to characterize the molecular phenotype of HEXIM knockdown during eye differentiation. HEXIM knockdown during eye differentiation was carried out with a UAS-Hexim-RNAi strain crossed with a GMR-Gal4 driver strain (Bloomington stock center)). Adult GMR>RNAi Hexim flies display slightly smaller, rough and often necrosed eyes. Total RNA were collected from isolated heads. We then used microarray to compare the transcriptome of HEXIM knockdown mutant with that of wild type flies.
CG10407 DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
Rala UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
Ady43A DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
CG32553 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
CG13101 DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
(Factor value) GENOTYPE/wild type genotype
rst DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
Osi6 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
AttA UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
CG10924 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
(Sample) genotype/HEXIM knockdown mutant
Cpr49Af DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
(Factor value) GENOTYPE/HEXIM knockdown mutant
CG9150 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
Obp56f DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
mre11 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
CG15117 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
(Sample) organism/Drosophila melanogaster
tilB UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
RFeSP DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'
CG7906 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
PGRP-SA UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
CecC UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
CG8620 UP in 'HEXIM knockdown mutant' vs 'wild type genotype'
Rh5 DOWN in 'HEXIM knockdown mutant' vs 'wild type genotype'