Rgs16 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Insl5 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Mucl1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
(Sample) cell type/intestinal epithelial cell
Retnlb DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ppy UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Snrpn UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Wee1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Pyy UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Fxyd3 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Calcb DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Myof DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Dlg1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Anapc1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Thbs1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Timp3 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Rhoq DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Plet1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Bend7 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gjb3 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ano1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Lpo UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cxcl13 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Kit DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gchfr UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Sycn DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gml UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
A4gnt DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ccl9 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
The development and severity of inflammatory bowel diseases (IBD) and other chronic inflammatory conditions can be influenced by host genetic and environmental factors, including signals derived from commensal bacteria. However, the mechanisms that integrate these diverse cues remain undefined. Here we demonstrate that intestinal epithelial cells (IECs) isolated from IBD patients exhibit decreased expression of the epigenome-modifying enzyme histone deacetylase 3 (HDAC3). Further, genome-wide analyses of murine IECs that lack HDAC3 (HDAC3ÃÂIEC) revealed that HDAC3 deficiency resulted in dysregulated gene expression coupled with alterations in histone acetylation. Critically, conventionally-housed HDAC3ÃÂIEC mice demonstrated loss of Paneth cells, impaired IEC function and alterations in the composition of intestinal commensal bacteria. In addition, HDAC3ÃÂIEC mice exhibited significantly increased susceptibility to intestinal damage and inflammation, indicating that epithelial expression of HDAC3 plays a central role in maintaining intestinal homeostasis. Strikingly, rederivation of HDAC3ÃÂIEC mice into germ-free conditions revealed that dysregulated IEC gene expression, Paneth cell homeostasis, and intestinal barrier function were largely restored in the absence of commensal bacteria. Collectively, these data indicate that the HDAC3 is a critical factor that integrates commensal bacteria-derived signals to calibrate epithelial cell responses required to establish normal host-commensal relationships and maintain intestinal homeostasis. In this study, we performed gene expression profiling to examine how the transcriptional profiles in primary live, EpCAM+ IECs from the large intestine differed between germ-free control HDAC3FF mice (3 biological replicates) and germ-free IEC-intrinsic knockout HDAC3ÃÂIEC mice (3 biological replicates).
Capg UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Tmem176b DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Prap1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ttr DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Zfp239 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ces1g DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Mttp UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gramd1b DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Wfdc18 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cmtm8 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
(Factor value) GENOTYPE/floxed HDAC3 (wild type)
Srd5a2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
4931406C07Rik UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Aadac DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Tbl1xr1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
1700057G04Rik UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gpt UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Sema7a UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Analysis of 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Slc25a45 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Phlda1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Mettl7a1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Slc10a2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cpq DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Slc9a3 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gm14137 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gjb5 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Lgals2 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gdf15 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Me1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Anxa13 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cbr3 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Pdk4 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Coq5 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Itch DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Iapp UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
St3gal5 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Hnf4g DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Fgfr2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Lpcat4 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Sptssb UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Il1rn UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Hpd DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
1700016C15Rik UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gbe1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ddah1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Mgll UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gm10680 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Il1r1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gm21847 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ces1d DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
S100a14 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
St6gal1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
C1qa DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Eno3 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Pdia5 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Areg UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Muc4 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ndrg1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gch1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
St3gal4 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Creb3l3 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Vnn1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Akr1c14 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Fam57a UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Pnliprp2 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Mptx1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Pbp2 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cyp4f16 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
(Sample) genotype/IEC-specific HDAC3 deletion
Hsd11b2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gsdmc2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Scd1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
(Factor value) GENOTYPE/IEC-specific HDAC3 deletion
Vsig2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gm1123 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Sst UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
2210407C18Rik UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Hpgd DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Apoc2 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gm10561 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
BC021614 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cytip DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cdc42ep2 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gm6086 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Mal DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cyp2c68 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ero1l UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Cers4 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ttyh1 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Slfn4 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Dusp6 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Sdc2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Bche DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Sult1c2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Eid1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Slc37a2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Gm8074 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ephx2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
H2-T3 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Epha2 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Sult1a1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Pik3r3 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ubd UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Plcb1 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Rbp7 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Depdc7 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Akr1b8 UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Pls3 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Mboat2 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Ckb UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Apol10a DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Dfna5 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
Mier3 DOWN in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'
(Sample) genotype/floxed HDAC3 (wild type)
Ereg UP in 'IEC-specific HDAC3 deletion' vs 'floxed HDAC3 (wild type)'