Mllt11 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Cdh6 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Slit2 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Pax6 DOWN in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Sulf1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Nefl UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Analysis of '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
(Sample) developmental stage/embryoid bodies
Sfrp2 DOWN in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Spon1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Elavl4 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Gja1 DOWN in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Insm1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
(Factor value) COMPOUND/500 nanomolar retinoic acid
Fam102b UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Ndnf DOWN in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Ntn1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Nefm UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Isl1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Nkx2-9 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Nnat UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Gap43 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
(Factor value) COMPOUND/500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist
500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist
Stmn2 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Olig1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Dbx1 DOWN in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Chgb UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
This experiment was specifically designed to measure neural targets of Shh signaling, we sought to profile the genes upregulated by Hh signaling in the ventral neural tube to obtain a valid dataset. To obtain ventral-specific markers, we generated retinoic acid-treated EBs grown in the presence or absence of HH-Ag. We did not observe induction of ventral Hh markers in RA-treated constitutive Gli1FLAG EBs and used these for the control, baseline set. The presence of FoxA2, Nkx2.9 and Nkx6.1 amongst the top 10 genes based on expression levels suggests that profiling significantly enriches for Hh-dependent cell types. As expected, the benchmark standard Gli1 was not up-regulated in our array, since it is constitutively expressed in the control as well. Experiment Overall Design: There are a total of 8 samples. Four biological replicates of Retinoic-acid treated EBs (Baseline) and 4 additional biological samples of retinoic acid + Hh-Ag treated EBs (induced sample).
Irx3 DOWN in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Hoxb5os DOWN in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Crmp1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Prnp UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Nkx6-1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Arg1 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Rgs4 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Foxa2 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'
Tubb3 UP in '500 nanomloar retinoic acid and 1 micromolar Hedgehog agonist' vs '500 nanomolar retinoic acid'