ATF3 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
SIPA1L2 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
CYP1B1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
CXCL3 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
NFATC2 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
ROR1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
PTGS2 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
DLG1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
SHISA2 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
NPTX1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
CCL2 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
SELE UP in 'cultured with ultrafine air pollutant particles' vs 'control'
CXCL8 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
F3 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
(Assay) endothelial cell_control_rep3(55)
SERPINB2 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
TIPARP UP in 'cultured with ultrafine air pollutant particles' vs 'control'
SECTM1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
(Assay) endothelial cell_control_rep1(53)
ACKR3 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
CYP1A1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
Analysis of 'cultured with ultrafine air pollutant particles' vs 'control'
(Assay) endothelial cell_UFP treatment_rep4(60)
(Assay) endothelial cell_control_rep4(56)
Epidemiology studies have linked exposure to pollutant particles to increased cardiovascular mortality and morbidity, however, the mechanism remains unknown. In this study, we hypothesized that the ultrafine fraction of ambient pollutant particles would cause endothelial cells dysfunction. We profiled gene expression of human pulmonary artery endothelial cells (HPAEC) exposed to ultrafine Chapel Hill particles (UFP) (100ÃÂÃÂ¼g/ml) or vehicle for 4h with Affymetrix HG U133 Plus 2.0 chips (N = 4 each). Using an unpaired t-test (p <0.01, 5% false discovery rate) we found 426 unique genes to be differentially expressed with 320 upregulated genes and 106 downregulated genes. Among these genes, we noted upregulation of genes related to coagulation-inflammation circuitry including tissue factor (F3), coagulation factor II receptor-like 2 (F2RL2, PAR3), interleukin (IL)-6 and IL-8. Upregulation of these genes were independently confirmed by RT-PCR and/or protein release. Genes related to the CXC chemokine family that have been implicated in the pathogenesis of vascular disease were upregulated, including MCP-1 (2.60 fold), IL-8 (2.47 fold), CXCL1 (1.41 fold), CXCL2 (1.95 fold), CXCL3 (2.28 fold) and CXCR4 (1.30 fold). In addition, genes related to clotting independent signaling of F3 were also differentially expressed, including FOS, JUN and NFKBIA. Treatment of HPAEC with UFP for 16 hours increased the release of IL6 and IL8 by 1.9-fold and 1.8-fold respectively. Pretreatment of HPAEC with a blocking antibody against F3 attenuated IL6 and IL8 release by 30% and 70% respectively. Thus using gene profiling, we uncovered that UFP may induce vascular endothelial cells to express genes related to clotting and angiogenesis. These results provide a novel hypothesis that PM may cause cardiovascular adverse health effects via induction of tissue factor in vascular endothelial cells which then triggers clotting dependent and independent downstream signaling. Experiment Overall Design: Human pulmonary artery endothelial cell cultures were treated with Chapel Hill Ultrafine particles or with vehicle control for 4h. 4 bological replicates each for treatment (100ug/ml) and control. 8 affy chips total.
(Assay) endothelial cell_UFP treatment_rep1(57)
(Assay) endothelial cell_UFP treatment_rep3(59)
SLC30A1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
GCLM UP in 'cultured with ultrafine air pollutant particles' vs 'control'
TNFRSF11A UP in 'cultured with ultrafine air pollutant particles' vs 'control'
SOCS3 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
(Assay) endothelial cell_control_rep2(54)
CEBPD UP in 'cultured with ultrafine air pollutant particles' vs 'control'
APOLD1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
AHRR UP in 'cultured with ultrafine air pollutant particles' vs 'control'
SLC16A6 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
(Assay) endothelial cell_UFP treatment_rep2(58)
CXCL2 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
(Factor value) GROWTH_CONDITION/cultured with ultrafine air pollutant particles
cultured with ultrafine air pollutant particles
IL6 UP in 'cultured with ultrafine air pollutant particles' vs 'control'
HGF UP in 'cultured with ultrafine air pollutant particles' vs 'control'
HMOX1 UP in 'cultured with ultrafine air pollutant particles' vs 'control'