Dcun1d4 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ccdc47 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rora DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mad2l2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ncs1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Gas2l3 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ttpal UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
1700007K13Rik UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Anln DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ralgps2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Zdhhc21 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ankrd1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Plk1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tgfbr1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Igf2bp2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Crebzf UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Foxm1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Parm1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mthfd2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cenpp DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Emp2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Parpbp DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mctp2 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Aurka DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tmem170b DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Serpina1b UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Plk4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Slc40a1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ano3 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Slc10a7 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ifitm1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Aurkb DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Gm16039 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Dhrs3 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tgfb2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
(Factor value) RNA_INTERFERENCE/scrambled control
Sesn1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ncapg DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rnf152 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Gys1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cdc20 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Pcmt1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Pik3r3 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Sapcd2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Man2a1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Etv5 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Hmmr DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Frmd6 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cttnbp2nl DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Gpd1l DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
AI464131 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Apmap UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Fut11 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cenpl DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Atoh8 is a transcription factor of the basic-helix-loop-helix (bHLH) family that is expressed in multiple tissues during embryonic development but whose specific functions remain unknown. The gene encoding Atoh8 is induced by various lineage- determining bHLH transcription factors in cell culture, suggesting a possible common role of this gene in multiple bHLH-driven differentiation programs. In the pancreas, the pro-endocrine bHLH factors Neurogenin3 (Neurog3) and NeuroD1 activate Atoh8 expression. Moreover, Atoh8 is expressed in the embryonic pancreas confirming its participation in the pancreatic transcriptional cascade. This work aims at gaining insight into the molecular function of Atoh8 during the endocrine differentiation program initiated by Neurog3 in the pancreas. To this aim, we have generated a recombinant adenovirus encoding an Atoh8-specific shRNA (Ad-shAtoh8) and used it to down-regulate expression of the Atoh8 gene in Neurog3-expressing pancreatic ductal cells (mPAC), a cellular model of endocrine cell differentiation. Thus, we have compared global changes in gene expression profiles between cells treated with Ad-Neurog3+shControl and cells treated with Ad-Neurog3+shAtoh8 using Affymetrix microarrays. Our results show that Atoh8 silencing significantly affects the expression of 293 genes in Neurog3-expressing mPAC cells. Gene Ontology analysis has revealed cell cycle as the biological function most significantly represented among the modified genes. These results uncover a potential function of Math6 as a regulator of cell cycle progression and provide novel insights into the link between Neurog3 and the regulation of the cell cycle. mPAC cells (mouse pancreatic duct cells) overexpressing Neurogenin3 were divided in 2 groups according to the shRNA encoded by the recombinant adenoviruses used: 1) Scramble shRNA (control for the infection effect), 2) Atonal Homolog 8-specific shRNA. Experiment was performed three independent times (3 independent biological replicates).
Knstrn DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tmcc2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tmem9b DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Nrm DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cnn2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mfsd1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ccnb2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Iqgap3 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Prc1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Arl4a UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ak4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Slc26a2 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cxcl15 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ero1l DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cenpi DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Blmh DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cep55 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cck UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Asah1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Psat1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ldlr DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Lrg1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Serpina1c UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cenpa DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Epha7 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Fstl3 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Sec24a UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Atf1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Dbi DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Hmgcs1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ckap4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Atad2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Bub1b DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mreg UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Afap1l2 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Smarce1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Znfx1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Sprr2h UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Crem UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tapbp DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Itgb6 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Sertad4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Capn2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mis18bp1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Abhd5 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mpzl2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Bub1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Snap23 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Lifr DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Trp53i11 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Asb13 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Nuf2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Fam60a DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Marveld2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cenpf DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Sgol1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Snx6 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Slk UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Snx4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Analysis of 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Erich2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Pign UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Iapp DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Neat1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Yipf6 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Sphkap UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ube2t DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Dennd1b DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rab8b DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rcn1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
P4ha2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ndc80 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Arl5b DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Celf5 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Uhrf1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tpx2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Aspm DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ttk DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Psph DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Trib3 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mastl DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Plekhg1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Pfkp DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tacc3 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Serpine1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Spag5 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Pglyrp1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Glipr2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cenpe DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Racgap1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Nedd4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Reg4 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ncapd2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Bambi UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Farsb DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Kif22 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Sgms2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Dpyd UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Egr1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ube3a DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Kif20a DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Trp53inp1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Maf DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Gdf10 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Depdc1a DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Lig4 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Eif3k DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ccna2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
1810026J23Rik UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Abcc4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Asns DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Slc1a4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Kif23 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rwdd4a DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Malat1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Fam64a DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Shisa2 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Pcgf3 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Glrb DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Setd7 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cdk1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rnase1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Lancl2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
9230114K14Rik UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ube2m UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tbc1d20 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Dmkn DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Gpt2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ncapg2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mitf UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Tk1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Prrx2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Chac1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rad51 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Hsbp1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mgarp DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cdca8 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rcan2 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ppp1ca DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Agpat5 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rnf181 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Fam101b DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Nap1l3 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Abcc1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ms4a10 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Scamp1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
(Factor value) RNA_INTERFERENCE/Atonal Homolog 8 shRNA
Slc5a3 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Nusap1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Hdac4 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
B230219D22Rik DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Alg10b DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Stbd1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Nrarp DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Mki67 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Slc35g1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Lonrf1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Prune2 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cand1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Ostm1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cdca2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Phtf2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Kif11 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Rock2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Bdh2 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cpped1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Kntc1 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Top2a DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
D030056L22Rik DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Crnkl1 UP in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Stard3nl DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Cfl2 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'
Slc7a11 DOWN in 'Atonal Homolog 8 shRNA' vs 'scrambled control'