ATSUC7 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
CRRSP4 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATSBT5.2 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G76800 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATOCT3 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AtRLP7 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G21890 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G59480 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ACS7 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATGDU2 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
DOT1 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATMES13 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G62480 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G48670 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G08990 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
MIOX2 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G66100 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AtSIP2 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
WAK1 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
SUC1 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G29450 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G41810 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AGL14 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
CYP81D8 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G05160 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
Analysis of 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G31860 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
WRKY18 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G32480 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT5G18840 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G51890 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G26420 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
UVR3 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G08410 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G29120 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G24980 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
MIOX4 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G27670 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G17300 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G11450 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
Rapamycin-sensitive transgenic Arabidopsis lines (BP12) expressing yeast FK506 Binding Protein12 (FKBP12) were developed. Inhibition of TOR in BP12 plants by rapamycin resulted in slower overall root, leaf and shoot growth and development leading to poor nutrient uptake and light energy utilization. Genetic and physiological studies together with RNA-Seq and metabolite analysis of TOR-suppressed lines revealed that TOR regulates development and lifespan in Arabidopsis by restructuring cell growth, carbon and nitrogen metabolism, gene expression, ribosomal RNA and protein synthesis. Arabidopsis WT (Col)and BP12-2 (TOR knockdown line) seedlings at 15 DAG were treated with rapamycin for 3 days by transferring from 0.5 MS medium to 0.5 MS+10 ug/ml rapamycin. Triplicate samples of rapamycin treated WT and BP12-2 seedlings were used for RNA-Seq analysis (Illumina Hiseq 2000). Paired-end alignments were obtained through aligning short reads onto the reference Arabidopsis Genome (TAIR9) using Bowtie. More than 80% of the reads mapped onto the genome. Htseq-count was used to count the reads from the Bowtie derived output files. Differential expressed genes were identified using edgeR. The FDR-corrected P value for differential expression was set to be <=0.05.
AT1G32928 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
TPS04 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
CYP705A20 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G27020 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
DTXL2 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATC UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G26380 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AtRLP24 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
CML11 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G13710 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATMES8 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
HR4 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATEXPB3 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
(Sample) developmental stage/15-day old seedling
AT3G26760 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ERD5 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
LAC2 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G08400 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G19460 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G61340 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G34520 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G28420 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
JAL22 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G01110 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
MDHAR UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G28990 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G21520 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
WRKY70 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AtbZIP48 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
RALFL27 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G11890 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G63055 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G53980 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G04500 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
XBAT34 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G12490 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
MAKR4 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G51820 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G25190 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ZEU1 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT5G24200 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G17740 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
(Sample) genotype/Rapamycin-sensitive TOR knockdown (BP12-2)
Rapamycin-sensitive TOR knockdown (BP12-2)
AT3G46490 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ICS2 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G14160 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
(Factor value) GENOTYPE/Rapamycin-sensitive TOR knockdown (BP12-2)
AT3G28540 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G04220 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G37870 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G62770 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
BHLH129 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G22540 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G40250 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G27590 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G28900 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G26390 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G01345 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
GH3.3 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
HB-5 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
LOG2 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
CYP82C4 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
UCC3 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G38560 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G39320 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G26610 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G14780 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATGSTF7 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G03700 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
RHS15 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G18200 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G25400 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
ATRL5 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
(Factor value) GENOTYPE/wild type genotype
AT4G08093 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
PAD3 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AtRLP22 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G73805 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT5G67430 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
BAP1 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
UGT76E4 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT3G59250 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G14190 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G65500 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
GAD4 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G25150 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
SP1L5 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G37900 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT4G37220 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
PGL5 DOWN in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT1G32920 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'
AT2G44370 UP in 'Rapamycin-sensitive TOR knockdown (BP12-2)' vs 'wild type genotype'