TNFAIP6 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -2.46510390155568
p value: 8.51784534280753E-7
refers to: 206026_s_at
refers to: TNFAIP6
t statistic: -21.0001494861877

(Assay) GSM882361
type: Assay
has participant: A-AFFY-44

220542_s_at
type: EFO_0005072
part of: A-AFFY-44

(Assay) GSM882379
type: Assay
has participant: A-AFFY-44

PMEPA1
type: EnsemblDatabaseReference

PMEPA1
type: EnsemblDatabaseReference

203395_s_at
type: EFO_0005072
part of: A-AFFY-44

PMEPA1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.78842066771362
p value: 5.41500683919887E-7
refers to: PMEPA1
refers to: 217875_s_at
t statistic: -22.219536591967

(Factor value) CELL_LINE/H460a
type: EFO_0003044
property type: CELL_LINE
property value: H460a

TM4SF20
type: EnsemblDatabaseReference

(Sample) cell line/A549
type: EFO_0001086
property type: cell line
property value: A549

TM4SF20
type: EnsemblDatabaseReference

(Assay) GSM882357
type: Assay
has participant: A-AFFY-44

Analysis of 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: MicroarrayDifferentialExpressionAnalysis

(Assay) GSM882367
type: Assay
has participant: A-AFFY-44

TNFAIP6
type: EnsemblDatabaseReference

(Factor value) TIME/6
type: EFO_0000001
property type: TIME
property value: 6

203394_s_at
type: EFO_0005072
part of: A-AFFY-44

SPOCK1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.05869031061451
p value: 3.68979958406114E-5
refers to: 202363_at
refers to: SPOCK1
t statistic: -13.0432335461227

(Assay) GSM882372
type: Assay
has participant: A-AFFY-44

(Assay) GSM882362
type: Assay
has participant: A-AFFY-44

SERPINE1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.57857293910658
p value: 1.65766463509434E-6
refers to: SERPINE1
refers to: 202628_s_at
t statistic: -19.2784974161479

EGFR
type: EnsemblDatabaseReference

EGFR
type: EnsemblDatabaseReference

(Assay) GSM882378
type: Assay
has participant: A-AFFY-44

A-AFFY-44
type: EFO_0000269

230813_at
type: EFO_0005072
part of: A-AFFY-44

F2RL1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.02793569675125
p value: 3.10135792529136E-4
refers to: F2RL1
refers to: 206429_at
t statistic: -9.81065460896968

COL1A1
type: EnsemblDatabaseReference

Analysis of 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'H460a' at '6 hour'
type: MicroarrayDifferentialExpressionAnalysis

(Assay) GSM882369
type: Assay
has participant: A-AFFY-44

F2RL1
type: EnsemblDatabaseReference

COL5A1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.00432449274492
p value: 3.28481456278265E-5
refers to: 212488_at
refers to: COL5A1
t statistic: -13.7325779509483

DNAJB9
type: EnsemblDatabaseReference

202843_at
type: EFO_0005072
part of: A-AFFY-44

DNAJB9
type: EnsemblDatabaseReference

KCNJ6
type: EnsemblDatabaseReference

KCNJ6
type: EnsemblDatabaseReference

(Assay) GSM882377
type: Assay
has participant: A-AFFY-44

RBP4
type: EnsemblDatabaseReference

(Assay) GSM882366
type: Assay
has participant: A-AFFY-44

1565483_at
type: EFO_0005072
part of: A-AFFY-44

(Factor value) TIME/24
type: EFO_0000001
property type: TIME
property value: 24

(Factor value) COMPOUND/gamma secretase inhibitor RO4929097
type: EFO_0000001
property type: COMPOUND
property value: gamma secretase inhibitor RO4929097

(Factor value) CELL_LINE/A549
type: EFO_0001086
property type: CELL_LINE
property value: A549

(Assay) GSM882382
type: Assay
has participant: A-AFFY-44

220639_at
type: EFO_0005072
part of: A-AFFY-44

1556499_s_at
type: EFO_0005072
part of: A-AFFY-44

BPIFA1
type: EnsemblDatabaseReference

BPIFA1
type: EnsemblDatabaseReference

(Assay) GSM882376
type: Assay
has participant: A-AFFY-44

(Assay) GSM882370
type: Assay
has participant: A-AFFY-44

(Assay) GSM882359
type: Assay
has participant: A-AFFY-44

TNFAIP6
type: EnsemblDatabaseReference

(Assay) GSM882375
type: Assay
has participant: A-AFFY-44

HES1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.85524100043007
p value: 1.10327118395168E-5
refers to: HES1
refers to: 203395_s_at
t statistic: -15.7744417841036

HES1
type: EnsemblDatabaseReference

HES1
type: EnsemblDatabaseReference

(Assay) GSM882371
type: Assay
has participant: A-AFFY-44

Homo sapiens
type: OBI_0100026

F2RL1
type: EnsemblDatabaseReference

EGFR DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '6 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.15707759511508
p value: 0.0296418983394477
refers to: EGFR
refers to: 1565483_at
t statistic: -4.29324517053293

(Assay) GSM882360
type: Assay
has participant: A-AFFY-44

206924_at
type: EFO_0005072
part of: A-AFFY-44

DNAJB9 UP in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'H460a' at '6 hour'
type: IncreasedDifferentialExpressionRatio
fold change: 1.25351301067259
p value: 0.00382086722216955
refers to: DNAJB9
refers to: 202843_at
t statistic: 10.7149681135094

SERPINE1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.13674099787692
p value: 2.21361849082319E-5
refers to: SERPINE1
refers to: 202627_s_at
t statistic: -14.400988489316

C3orf80
type: EnsemblDatabaseReference

PMEPA1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.73689462143005
p value: 1.40808761335335E-7
refers to: PMEPA1
refers to: 222450_at
t statistic: -29.6957086596359

(Assay) GSM882387
type: Assay
has participant: A-AFFY-44

SPOCK1
type: EnsemblDatabaseReference

SPOCK1
type: EnsemblDatabaseReference

212488_at
type: EFO_0005072
part of: A-AFFY-44

TM4SF20 UP in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: IncreasedDifferentialExpressionRatio
fold change: 1.48087297251337
p value: 2.07464047686159E-5
refers to: TM4SF20
refers to: 220639_at
t statistic: 14.6740235128238

(Sample) cell line/H460a
type: EFO_0003044
property type: cell line
property value: H460a

(Assay) GSM882368
type: Assay
has participant: A-AFFY-44

SERPINE1
type: EnsemblDatabaseReference

LEPREL1
type: EnsemblDatabaseReference

LEPREL1
type: EnsemblDatabaseReference

PMEPA1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.7432558428271
p value: 1.62644629336247E-7
refers to: PMEPA1
refers to: 222449_at
t statistic: -26.7171794416514

IL11 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.12042368036156
p value: 0.0359400917444298
refers to: 206924_at
refers to: IL11
t statistic: -4.08010936172544

LEPREL1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '6 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.53019141111085
p value: 0.0189661565455312
refers to: LEPREL1
refers to: 230813_at
t statistic: -5.55585159150868

(Assay) GSM882373
type: Assay
has participant: A-AFFY-44

(Assay) GSM882358
type: Assay
has participant: A-AFFY-44

TNFAIP6 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.71811431765745
p value: 3.22367427005438E-7
refers to: 206025_s_at
refers to: TNFAIP6
t statistic: -24.2069887621156

206429_at
type: EFO_0005072
part of: A-AFFY-44

(Assay) GSM882386
type: Assay
has participant: A-AFFY-44

COL5A1
type: EnsemblDatabaseReference

COL5A1
type: EnsemblDatabaseReference

COL1A1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.12117670742438
p value: 1.45624925356092E-6
refers to: COL1A1
refers to: 1556499_s_at
t statistic: -19.729075972005

(Assay) GSM882381
type: Assay
has participant: A-AFFY-44

222450_at
type: EFO_0005072
part of: A-AFFY-44

(Assay) GSM882363
type: Assay
has participant: A-AFFY-44

RBP4 UP in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: IncreasedDifferentialExpressionRatio
fold change: 1.27534469301032
p value: 3.60448095655756E-7
refers to: RBP4
refers to: 219140_s_at
t statistic: 23.4679385658978

HES1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -2.18312936260539
p value: 1.62644629336247E-7
refers to: HES1
refers to: 203394_s_at
t statistic: -26.4819641680808

232411_at
type: EFO_0005072
part of: A-AFFY-44

IL11
type: EnsemblDatabaseReference

HES1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '6 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.24537651440435
p value: 3.23678093372602E-5
refers to: HES1
refers to: 203395_s_at
t statistic: -11.6674595375697

IL11
type: EnsemblDatabaseReference

(Assay) GSM882374
type: Assay
has participant: A-AFFY-44

206026_s_at
type: EFO_0005072
part of: A-AFFY-44

C3orf80
type: EnsemblDatabaseReference

KCNJ6 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.02986088762669
p value: 0.00142123857745668
refers to: KCNJ6
refers to: 232411_at
t statistic: -7.55493081515224

(Assay) GSM882385
type: Assay
has participant: A-AFFY-44

206025_s_at
type: EFO_0005072
part of: A-AFFY-44

219140_s_at
type: EFO_0005072
part of: A-AFFY-44

BPIFA1 UP in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: IncreasedDifferentialExpressionRatio
fold change: 1.37826723692688
p value: 9.51924355497692E-5
refers to: BPIFA1
refers to: 220542_s_at
t statistic: 11.4001834660137

202363_at
type: EFO_0005072
part of: A-AFFY-44

Analysis of 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '6 hour'
type: MicroarrayDifferentialExpressionAnalysis

E-GEOD-36176
type: microarray 1colour mrna differential
RO_0002162: Homo sapiens
description: Genes responses in A549 and H460 cells after GSI (RO4929097-001-003 , 2 uM) treatment. Notch signaling is an area of great interest in oncology. RO4929097 is a potent and selective inhibitor of gamma-secretase, producing inhibitory activity of Notch signaling in tumor cells. The RO4929097 IC50 in cell-free and cellular assays is in the low nanomolar range with >100-fold selectivity with respect to 75 other proteins of various types (receptors, ion channels, and enzymes). RO4929097 inhibits Notch processing in tumor cells as measured by the reduction of intracellular Notch expression by Western blot. This leads to reduced expression of the Notch transcriptional target gene Hes1. RO4929097 does not block tumor cell proliferation or induce apoptosis but instead produces a less transformed, flattened, slower-growing phenotype. RO4929097 is active following oral dosing. Antitumor activity was shown in 7 of 8 xenografts tested on an intermittent or daily schedule in the absence of body weight loss or Notch-related toxicities. Importantly, efficacy is maintained after dosing is terminated. Angiogenesis reverse transcription-PCR array data show reduced expression of several key angiogenic genes. In addition, comparative microarray analysis suggests tumor cell differentiation as an additional mode of action. These preclinical results support evaluation of RO4929097 in clinical studies using an intermittent dosing schedule. A multicenter phase I dose escalation study in oncology is under way. A549 and H460 cells were treated in quadruplicate with either vehicle or GSI (RP4929097-001-003, 2 uM) for 6 or 24 hours.
uses data from: http://identifiers.org/pubmed/19773430

202628_s_at
type: EFO_0005072
part of: A-AFFY-44

C3orf80 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '24 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.55126731242343
p value: 4.6512245826639E-4
refers to: 236738_at
refers to: C3orf80
t statistic: -9.13336913625113

(Assay) GSM882388
type: Assay
has participant: A-AFFY-44

217875_s_at
type: EFO_0005072
part of: A-AFFY-44

(Assay) GSM882380
type: Assay
has participant: A-AFFY-44

(Assay) GSM882364
type: Assay
has participant: A-AFFY-44

SERPINE1
type: EnsemblDatabaseReference

202627_s_at
type: EFO_0005072
part of: A-AFFY-44

(Assay) GSM882384
type: Assay
has participant: A-AFFY-44

236738_at
type: EFO_0005072
part of: A-AFFY-44

222449_at
type: EFO_0005072
part of: A-AFFY-44

COL1A1
type: EnsemblDatabaseReference

(Assay) GSM882365
type: Assay
has participant: A-AFFY-44

(Sample) organism/Homo sapiens
type: Homo sapiens
property type: organism
property value: Homo sapiens

HES1 DOWN in 'gamma secretase inhibitor RO4929097' vs 'vehicle' in 'A549' at '6 hour'
type: DecreasedDifferentialExpressionRatio
fold change: -1.63187367489831
p value: 4.376596883355E-7
refers to: HES1
refers to: 203394_s_at
t statistic: -16.4901402334995

RBP4
type: EnsemblDatabaseReference

(Assay) GSM882383
type: Assay
has participant: A-AFFY-44

(Factor value) COMPOUND/vehicle
type: EFO_0000732
property type: COMPOUND
property value: vehicle