F41E6.11 DOWN in 'gold nanoparticles' vs 'control'
lpr-3 DOWN in 'gold nanoparticles' vs 'control'
pqn-75 DOWN in 'gold nanoparticles' vs 'control'
F33E11.2 DOWN in 'gold nanoparticles' vs 'control'
ZC123.1 DOWN in 'gold nanoparticles' vs 'control'
xpo-2 DOWN in 'gold nanoparticles' vs 'control'
glh-1 DOWN in 'gold nanoparticles' vs 'control'
let-805 DOWN in 'gold nanoparticles' vs 'control'
cdc-37 DOWN in 'gold nanoparticles' vs 'control'
ell-1 DOWN in 'gold nanoparticles' vs 'control'
H11E01.3 DOWN in 'gold nanoparticles' vs 'control'
nos-3 DOWN in 'gold nanoparticles' vs 'control'
dnj-13 DOWN in 'gold nanoparticles' vs 'control'
Y17G7B.17 DOWN in 'gold nanoparticles' vs 'control'
myo-1 DOWN in 'gold nanoparticles' vs 'control'
Y37H9A.3 DOWN in 'gold nanoparticles' vs 'control'
dyn-1 DOWN in 'gold nanoparticles' vs 'control'
sca-1 DOWN in 'gold nanoparticles' vs 'control'
daf-5 DOWN in 'gold nanoparticles' vs 'control'
ppw-2 DOWN in 'gold nanoparticles' vs 'control'
ncl-1 DOWN in 'gold nanoparticles' vs 'control'
hil-4 DOWN in 'gold nanoparticles' vs 'control'
F11E6.3 DOWN in 'gold nanoparticles' vs 'control'
F52G3.1 DOWN in 'gold nanoparticles' vs 'control'
F57F4.4 DOWN in 'gold nanoparticles' vs 'control'
npp-8 DOWN in 'gold nanoparticles' vs 'control'
Y47D3B.6 DOWN in 'gold nanoparticles' vs 'control'
K10C2.1 DOWN in 'gold nanoparticles' vs 'control'
C45B2.1 UP in 'gold nanoparticles' vs 'control'
ani-1 DOWN in 'gold nanoparticles' vs 'control'
T22B11.4 DOWN in 'gold nanoparticles' vs 'control'
sec-23 DOWN in 'gold nanoparticles' vs 'control'
W08E12.3 DOWN in 'gold nanoparticles' vs 'control'
rsr-1 DOWN in 'gold nanoparticles' vs 'control'
unc-89 DOWN in 'gold nanoparticles' vs 'control'
ZK180.5 DOWN in 'gold nanoparticles' vs 'control'
F20B10.3 DOWN in 'gold nanoparticles' vs 'control'
C34F6.1 DOWN in 'gold nanoparticles' vs 'control'
tsp-14 DOWN in 'gold nanoparticles' vs 'control'
mcm-2 DOWN in 'gold nanoparticles' vs 'control'
sea-2 DOWN in 'gold nanoparticles' vs 'control'
Y69H2.3 DOWN in 'gold nanoparticles' vs 'control'
gbf-1 DOWN in 'gold nanoparticles' vs 'control'
fip-1 UP in 'gold nanoparticles' vs 'control'
Y8A9A.2 DOWN in 'gold nanoparticles' vs 'control'
zfh-2 DOWN in 'gold nanoparticles' vs 'control'
R07B1.9 DOWN in 'gold nanoparticles' vs 'control'
snrp-200 DOWN in 'gold nanoparticles' vs 'control'
col-41 DOWN in 'gold nanoparticles' vs 'control'
R03C1.1 DOWN in 'gold nanoparticles' vs 'control'
H18N23.2 DOWN in 'gold nanoparticles' vs 'control'
Y57G11C.9 DOWN in 'gold nanoparticles' vs 'control'
hecd-1 DOWN in 'gold nanoparticles' vs 'control'
K10D3.4 DOWN in 'gold nanoparticles' vs 'control'
eef-1A.2 DOWN in 'gold nanoparticles' vs 'control'
hlh-30 DOWN in 'gold nanoparticles' vs 'control'
lec-1 DOWN in 'gold nanoparticles' vs 'control'
gei-4 DOWN in 'gold nanoparticles' vs 'control'
F14H3.12 UP in 'gold nanoparticles' vs 'control'
unc-51 DOWN in 'gold nanoparticles' vs 'control'
larp-5 DOWN in 'gold nanoparticles' vs 'control'
npp-11 DOWN in 'gold nanoparticles' vs 'control'
ZC204.1 UP in 'gold nanoparticles' vs 'control'
ketn-1 DOWN in 'gold nanoparticles' vs 'control'
Y38C9A.1 DOWN in 'gold nanoparticles' vs 'control'
T22F3.3 DOWN in 'gold nanoparticles' vs 'control'
imb-3 DOWN in 'gold nanoparticles' vs 'control'
pqn-54 DOWN in 'gold nanoparticles' vs 'control'
K04H4.2 DOWN in 'gold nanoparticles' vs 'control'
pha-4 DOWN in 'gold nanoparticles' vs 'control'
tfg-1 DOWN in 'gold nanoparticles' vs 'control'
nep-17 DOWN in 'gold nanoparticles' vs 'control'
Y47D7A.13 DOWN in 'gold nanoparticles' vs 'control'
F14F9.6 UP in 'gold nanoparticles' vs 'control'
T23E7.2 DOWN in 'gold nanoparticles' vs 'control'
F35A5.1 DOWN in 'gold nanoparticles' vs 'control'
sup-26 DOWN in 'gold nanoparticles' vs 'control'
rsa-2 DOWN in 'gold nanoparticles' vs 'control'
K08E3.4 DOWN in 'gold nanoparticles' vs 'control'
pqn-71 DOWN in 'gold nanoparticles' vs 'control'
lin-41 DOWN in 'gold nanoparticles' vs 'control'
Y55B1AR.2 DOWN in 'gold nanoparticles' vs 'control'
F49E2.5 DOWN in 'gold nanoparticles' vs 'control'
pqn-87 DOWN in 'gold nanoparticles' vs 'control'
F08D12.1 DOWN in 'gold nanoparticles' vs 'control'
F53F1.4 DOWN in 'gold nanoparticles' vs 'control'
C12D12.1 DOWN in 'gold nanoparticles' vs 'control'
gpb-1 DOWN in 'gold nanoparticles' vs 'control'
atx-2 DOWN in 'gold nanoparticles' vs 'control'
acs-2 DOWN in 'gold nanoparticles' vs 'control'
(Factor value) GROWTH_CONDITION/gold nanoparticles
ins-22 UP in 'gold nanoparticles' vs 'control'
unc-11 DOWN in 'gold nanoparticles' vs 'control'
We used Au nanoparticles (Au-NPs) as a model for studying particle specific effects of manufactured nanomaterials (MNMs) by examining the toxicogenomic responses in a model soil organism, free living nematode Caenorhabditis elegans. Global genome expression for nematodes exposed to 4-nm citrate-coated Au-NPs at the LC10 (5.9 mg L-1) revealed significant differential expression of 797 genes. The levels of expression for five genes (apl-1, dyn-1, act-5, abu-11, and hsp-4) were confirmed independently with qRT-PCR. Seven common biological pathways associated with 38 of these genes were identified. Activation of 26 pqn/abu genes from noncanonical Unfolded Protein Response (UPR) pathway and up-regulation of molecular chaperones (hsp-16.1, hsp-70, hsp-3 and hsp-4) were observed and are likely indicative of endoplasmic reticulum stress. Inhibition of abu-11 with RNAi showed increase in mortality in Au-NP exposed nematodes suggesting possible involvement of abu-11 (a gene associated with specific to C. elegans UPR) in a protective mechanism against Au-NPs. Exposure to Au-NPs also caused activation of genes involved in apoptosis and necrosis and resulted ultimately in 10% mortality. These results demonstrate that Au-NPs are bioavailable and cause adverse effects to a model ecoreceptor which activate both general and specific biological pathways. Age-synchronized L3 nematodes (about 2000 nematodes per replicate with 3 replicates per treatment) were exposed to LC10 (5.9 mgL-1 ) of 4-nm citrate-coated Au-NP using 6 cm petri-dishes with 4 ml of exposure solution per dish. The N2 nematodes at L3 stageused were used and they were exposed in 50% K-Medium (31.68 mM KCl, 51.37 mM NaCl) for 12h. At 12 h all solutions were replaced with 50% K-Medium and left for another 12 h. After 24 h RNA was extracted from each of the replicates .
tpxl-1 DOWN in 'gold nanoparticles' vs 'control'
mcm-4 DOWN in 'gold nanoparticles' vs 'control'
unc-52 DOWN in 'gold nanoparticles' vs 'control'
wsp-1 DOWN in 'gold nanoparticles' vs 'control'
ppk-2 DOWN in 'gold nanoparticles' vs 'control'
lin-66 DOWN in 'gold nanoparticles' vs 'control'
Analysis of 'gold nanoparticles' vs 'control'
cbd-1 DOWN in 'gold nanoparticles' vs 'control'
tln-1 DOWN in 'gold nanoparticles' vs 'control'
lea-1 DOWN in 'gold nanoparticles' vs 'control'
Y54G2A.26 DOWN in 'gold nanoparticles' vs 'control'
copb-1 DOWN in 'gold nanoparticles' vs 'control'
car-1 DOWN in 'gold nanoparticles' vs 'control'
oac-14 DOWN in 'gold nanoparticles' vs 'control'
grl-16 DOWN in 'gold nanoparticles' vs 'control'
gsa-1 DOWN in 'gold nanoparticles' vs 'control'
R04E5.8 DOWN in 'gold nanoparticles' vs 'control'
fbxa-163 UP in 'gold nanoparticles' vs 'control'
let-2 DOWN in 'gold nanoparticles' vs 'control'
gld-1 DOWN in 'gold nanoparticles' vs 'control'
vit-2 DOWN in 'gold nanoparticles' vs 'control'
pqn-32 DOWN in 'gold nanoparticles' vs 'control'
mig-6 DOWN in 'gold nanoparticles' vs 'control'
pod-2 DOWN in 'gold nanoparticles' vs 'control'
Y39E4A.3 DOWN in 'gold nanoparticles' vs 'control'
D1005.1 DOWN in 'gold nanoparticles' vs 'control'
par-2 DOWN in 'gold nanoparticles' vs 'control'
unc-73 DOWN in 'gold nanoparticles' vs 'control'
W04B5.3 DOWN in 'gold nanoparticles' vs 'control'
icl-1 DOWN in 'gold nanoparticles' vs 'control'
F49B2.6 DOWN in 'gold nanoparticles' vs 'control'
ZK669.3 UP in 'gold nanoparticles' vs 'control'
grl-4 DOWN in 'gold nanoparticles' vs 'control'
eel-1 DOWN in 'gold nanoparticles' vs 'control'
abu-11 DOWN in 'gold nanoparticles' vs 'control'
hsp-110 DOWN in 'gold nanoparticles' vs 'control'
T21B6.3 DOWN in 'gold nanoparticles' vs 'control'
(Sample) organism/Caenorhabditis elegans
hsp-60 DOWN in 'gold nanoparticles' vs 'control'
mex-5 DOWN in 'gold nanoparticles' vs 'control'
mlt-11 DOWN in 'gold nanoparticles' vs 'control'
zfp-3 DOWN in 'gold nanoparticles' vs 'control'
Y39B6A.1 DOWN in 'gold nanoparticles' vs 'control'
grd-6 DOWN in 'gold nanoparticles' vs 'control'
wwp-1 DOWN in 'gold nanoparticles' vs 'control'
ZK470.2 DOWN in 'gold nanoparticles' vs 'control'
sdha-1 DOWN in 'gold nanoparticles' vs 'control'
hcp-1 DOWN in 'gold nanoparticles' vs 'control'
pqn-74 DOWN in 'gold nanoparticles' vs 'control'
F45D11.1 UP in 'gold nanoparticles' vs 'control'
E01G6.1 DOWN in 'gold nanoparticles' vs 'control'
lin-42 DOWN in 'gold nanoparticles' vs 'control'
wip-1 DOWN in 'gold nanoparticles' vs 'control'
kin-2 DOWN in 'gold nanoparticles' vs 'control'
abu-14 DOWN in 'gold nanoparticles' vs 'control'
cpg-1 DOWN in 'gold nanoparticles' vs 'control'
noah-2 DOWN in 'gold nanoparticles' vs 'control'
cnc-1 UP in 'gold nanoparticles' vs 'control'
F31C3.3 DOWN in 'gold nanoparticles' vs 'control'
T24B8.4 DOWN in 'gold nanoparticles' vs 'control'
pod-1 DOWN in 'gold nanoparticles' vs 'control'
C05D12.3 DOWN in 'gold nanoparticles' vs 'control'
asns-1 DOWN in 'gold nanoparticles' vs 'control'
F09C8.2 DOWN in 'gold nanoparticles' vs 'control'
sqt-3 DOWN in 'gold nanoparticles' vs 'control'
nas-15 DOWN in 'gold nanoparticles' vs 'control'
ncx-2 DOWN in 'gold nanoparticles' vs 'control'
copb-2 DOWN in 'gold nanoparticles' vs 'control'
Y75B8A.24 DOWN in 'gold nanoparticles' vs 'control'
dpy-8 DOWN in 'gold nanoparticles' vs 'control'
myo-2 DOWN in 'gold nanoparticles' vs 'control'
R02F2.1 DOWN in 'gold nanoparticles' vs 'control'
spat-2 DOWN in 'gold nanoparticles' vs 'control'
F30H5.3 DOWN in 'gold nanoparticles' vs 'control'
pqn-13 DOWN in 'gold nanoparticles' vs 'control'
F32A7.5 DOWN in 'gold nanoparticles' vs 'control'
Y48G1C.7 DOWN in 'gold nanoparticles' vs 'control'
W05B5.1 DOWN in 'gold nanoparticles' vs 'control'
tts-2 DOWN in 'gold nanoparticles' vs 'control'
emb-9 DOWN in 'gold nanoparticles' vs 'control'
feh-1 DOWN in 'gold nanoparticles' vs 'control'
Y48E1B.2 DOWN in 'gold nanoparticles' vs 'control'
F38E9.5 DOWN in 'gold nanoparticles' vs 'control'
acdh-7 DOWN in 'gold nanoparticles' vs 'control'
C50F7.5 DOWN in 'gold nanoparticles' vs 'control'
tkt-1 DOWN in 'gold nanoparticles' vs 'control'
klp-20 DOWN in 'gold nanoparticles' vs 'control'
abu-10 DOWN in 'gold nanoparticles' vs 'control'
cut-2 DOWN in 'gold nanoparticles' vs 'control'
xpc-1 DOWN in 'gold nanoparticles' vs 'control'
rnp-8 DOWN in 'gold nanoparticles' vs 'control'
alg-2 DOWN in 'gold nanoparticles' vs 'control'