Pmepa1 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Evc DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Slc27a3 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Actc1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Kif5c UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Enho UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Rnd3 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Sln UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Junos DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Mstn DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Galr2 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Crip3 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Prima1 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Srrm4os UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Lmcd1 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Adamtsl1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Analysis of 'active Mstn exon 3 floxed' vs 'active wild type'
Gm21807 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Otud1 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Mss51 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Tmem100 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Panx1 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
RP24-481M4.1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Gm15564 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Nipa1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Aldh18a1 UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Gm11716 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Vgll2 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Atcay DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Grem2 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Mettl21e UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Il12a UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Katnal2 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
2610018G03Rik UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Gm4841 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
2310014F06Rik DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Analysis of 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Gprc5a UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Kcnab1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Gm21750 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Tmem86a DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Wnk2 UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Casr UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Cyp1a1 UP in 'active Mstn exon 3 floxed' vs 'active wild type'
BC023105 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Dkk3 DOWN in 'active Mstn exon 3 floxed' vs 'active wild type'
Habp2 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Myl10 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Yam1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Gm23935 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Per1 UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Gm12185 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
(Factor value) PHYSICAL_ACTIVITY/sedentary
Ddah1 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Mss51 DOWN in 'active Mstn exon 3 floxed' vs 'active wild type'
Gm830 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Panx1 DOWN in 'active Mstn exon 3 floxed' vs 'active wild type'
The purpose of this study was to determine whether postdevelopmental myostatin depletion influenced the changes in skeletal muscle gene expression profiles induced by a long-term increase in physical activity. Myostatin levels in muscles of adult male mice with floxed myostatin genes were reduced ~85% by activating Cre recombinase. Control mice with normal myostatin genes had the same Cre-activating treatment. Some of the mice were housed in ordinary cages throughout the study, limiting their physical activity. Other mice were given free access to running wheels for the final 12 weeks of the study. At the end of the study, comprehensive gene expression profiles of triceps brachii muscles were determined by RNA sequencing (RNA-Seq), with muscles from mice selected for similarity of running behavior throughout the period of wheel access. Wheel running increased expression of hundreds of mRNAs encoding proteins involved in oxidative energy metabolism, and this response was not affected by myostatin deficiency. The running-induced increase in the ratio of Myh1 mRNA (which encodes myosin heavy chain type 2x) to Myh4 mRNA (which encodes myosin heavy chain type 2b) also was not affected by myostatin depletion. At every threshold of P (computed by analysis of variance), the number of transcripts with interactions between activity level and myostatin level was fewer than the number expected by chance. These data suggest that myostatin is not required for transcriptional adaptations to moderate-intensity exercise. 12 samples, 6 from sedentary mice and 6 from active (wheel running) mice. 3 control and 3 myostatin-deficient mice within each activity level.
Dok5 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Katnal2 UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Mstn DOWN in 'active Mstn exon 3 floxed' vs 'active wild type'
Il12a UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Mir6236 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Ddah1 DOWN in 'active Mstn exon 3 floxed' vs 'active wild type'
Cyp26b1 UP in 'active Mstn exon 3 floxed' vs 'active wild type'
RP24-481M4.1 UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Tph1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Vash2 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Gm21769 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Hsd17b7 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Cdh4 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Lancl1 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Sln UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Dkk3 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
(Factor value) GENOTYPE/Mstn exon 3 floxed
Mettl21e UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
AI118078 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Vmn1r65 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Sdk1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Actc1 UP in 'active Mstn exon 3 floxed' vs 'active wild type'
Igfbp5 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Gm15662 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Fam19a5 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Lancl1 DOWN in 'active Mstn exon 3 floxed' vs 'active wild type'
Nr4a2 DOWN in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'
Ankrd1 DOWN in 'active Mstn exon 3 floxed' vs 'active wild type'
(Factor value) GENOTYPE/wild type genotype
Pak1 UP in 'sedentary Mstn exon 3 floxed' vs 'sedentary wild type'