AT3G46490 DOWN in 'pkl-1' vs 'wild type'
AT4G39675 DOWN in 'pkl-1' vs 'wild type'
AT1G66100 DOWN in 'pkl-1' vs 'wild type'
AT5G62280 DOWN in 'pkl-1' vs 'wild type'
AT3G51330 DOWN in 'pkl-1' vs 'wild type'
AT5G11230 DOWN in 'pkl-1' vs 'wild type'
AT2G39850 DOWN in 'pkl-1' vs 'wild type'
AT1G02820 DOWN in 'pkl-1' vs 'wild type'
BETA-OHASE 2 DOWN in 'pkl-1' vs 'wild type'
AT3G54500 DOWN in 'pkl-1' vs 'wild type'
AT5G38020 DOWN in 'pkl-1' vs 'wild type'
AT5G64170 DOWN in 'pkl-1' vs 'wild type'
CHD3 proteins are ATP-dependent chromatin remodeling factors that are components of diverse multisubunit complexes that can either repress or activate gene expression. In plants, the CHD3 protein PICKLE (PKL) is necessary for repression of seed-specific genes during germination and promotes deposition of the repressive epigenetic mark trimethylation of histone H3 lysine 27 (H3K27me3). It is unknown, however, if PKL acts directly at H3K27me3-enriched loci. We undertook a microarray analysis of 14-day-old plants and found that PKL continues to play an important role in expression of H3K27me3-enriched genes and in specification of developmental identity after germination. We used microarray to identify genes that are differentialy expressed in 14-day-old pkl seedlings and used chormatin immunoprecipitation to identify genes that are the direct targets of PKL. Wild-type (Col-0) and pkl-1 seedlings were grown on 1/2 MS plates with constant light and harvsted after 14-day growth. Three biological replicates.
AT1G66540 DOWN in 'pkl-1' vs 'wild type'
AT4G01440 DOWN in 'pkl-1' vs 'wild type'
AT1G67865 DOWN in 'pkl-1' vs 'wild type'
AT1G14960 DOWN in 'pkl-1' vs 'wild type'
AT2G39980 DOWN in 'pkl-1' vs 'wild type'
AT2G39030/T7F6.20 UP in 'pkl-1' vs 'wild type'
AT1G31690 DOWN in 'pkl-1' vs 'wild type'
AT3G44970 DOWN in 'pkl-1' vs 'wild type'
AT2G32160 DOWN in 'pkl-1' vs 'wild type'
AT4G11210 DOWN in 'pkl-1' vs 'wild type'
AT1G12080 DOWN in 'pkl-1' vs 'wild type'
AT2G16005 DOWN in 'pkl-1' vs 'wild type'
AT3G15760 DOWN in 'pkl-1' vs 'wild type'
AT1G32220 DOWN in 'pkl-1' vs 'wild type'
AT2G24160 DOWN in 'pkl-1' vs 'wild type'
AT1G22430 DOWN in 'pkl-1' vs 'wild type'
AT2G43590 DOWN in 'pkl-1' vs 'wild type'
AT1G55960 DOWN in 'pkl-1' vs 'wild type'
AT3G60070 DOWN in 'pkl-1' vs 'wild type'
AT3G14760 DOWN in 'pkl-1' vs 'wild type'
AT1G70985 DOWN in 'pkl-1' vs 'wild type'
AT1G26920 DOWN in 'pkl-1' vs 'wild type'
AT1G54890 DOWN in 'pkl-1' vs 'wild type'
AT4G33666 DOWN in 'pkl-1' vs 'wild type'
AT4G07820 DOWN in 'pkl-1' vs 'wild type'
FMO GS-OX4 DOWN in 'pkl-1' vs 'wild type'
AT2G23840 DOWN in 'pkl-1' vs 'wild type'
AT5G44260 DOWN in 'pkl-1' vs 'wild type'
AT4G08300 DOWN in 'pkl-1' vs 'wild type'
AT5G35970 DOWN in 'pkl-1' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
AT4G15620 DOWN in 'pkl-1' vs 'wild type'
AT4G28290 DOWN in 'pkl-1' vs 'wild type'
AT5G22390 DOWN in 'pkl-1' vs 'wild type'