Epas1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Adamts9 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Gpr133 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Trem1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Serpinb2 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
(Factor value) GENOTYPE/KLF2âÂÂ/âÂÂ
Rdh10 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Gene expression profile of LysMCre/Cre and KLF2âÂÂ/âÂÂ primary peritoneal macrophages following 6 hours of LPS treatment. We used microarrays to detail the global program of gene expression following LPS stimulation of LysMCre/Cre and KLF2âÂÂ/âÂÂ primary peritoneal macrophages. We identified distinct classes of genes that were altered following LPS stimulation. We injected 3 pairs of LysMCre/Cre (n=3) and KLF2âÂÂ/âÂÂ (n=3) mice with 2 ml of thioglycolate broth intraperitoneally. After 72 hours of thioglycolate injection, total cell suspension from peritoneal cavity were collected. These cells were seeded on 100mm tissue culture plates. Cells were allowed to attach for 4 hours and unattached cells were removed by washing with cell culture medium. These cells were placed in humidified incubator for additional 24 hours before LPS treatment. LPS was diluted to final concentration of 100ng/ml in culture medium and this medium was placed on peritoneal macrophages derived from LysM Cre/Cre and KLF2âÂÂ/âÂÂ mice for 6hours. Cells were lysed and total RNA was isolated and subjected to hybridization on Affymetrix microarrays.
Nckap1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Ptgs1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Ccdc80 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
(Sample) cell type/Thioglycolate elucidated primary peritoneal macrophage
Thioglycolate elucidated primary peritoneal macrophage
Cyr61 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Steap2 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Cxcl5 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Gfpt2 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Tead1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Cxcl13 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Vsig4 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Cyp1b1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Timp3 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Efna5 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Kdelr3 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Slit3 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Upk1b DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Steap4 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Nedd4 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Pdgfra DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Gm25360 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Cyp7b1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Ptgis DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Phldb2 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Clec4a3 UP in 'KLF2∆/∆' vs 'LysMCre/Cre'
Cxcl3 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Cxcl1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Slc16a2 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Fads2 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Cks1b DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Il1r1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Antxr1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
n-R5s88 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Steap1 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Lrrn4 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Wdr83os DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Clca2 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Fermt2 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Clec4a1 UP in 'KLF2∆/∆' vs 'LysMCre/Cre'
Igkv1-117 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Gabra3 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Mki67 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Igkv4-91 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Serpina3n DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Epha4 DOWN in 'KLF2∆/∆' vs 'LysMCre/Cre'
Slc13a3 UP in 'KLF2∆/∆' vs 'LysMCre/Cre'