Slc4a1 DOWN in 'Lkb1 knock out' vs 'wild type'
Ccl3 DOWN in 'Lkb1 knock out' vs 'wild type'
S100a8 DOWN in 'Lkb1 knock out' vs 'wild type'
Mmp8 DOWN in 'Lkb1 knock out' vs 'wild type'
Fn1 DOWN in 'Lkb1 knock out' vs 'wild type'
1100001G20Rik DOWN in 'Lkb1 knock out' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
Vnn1 DOWN in 'Lkb1 knock out' vs 'wild type'
(Sample) organism part/bone marrow from femoral and tibial bones
bone marrow from femoral and tibial bones
Pdcd1 UP in 'Lkb1 knock out' vs 'wild type'
Trib3 DOWN in 'Lkb1 knock out' vs 'wild type'
Greb1 DOWN in 'Lkb1 knock out' vs 'wild type'
F13a1 UP in 'Lkb1 knock out' vs 'wild type'
Chil3 DOWN in 'Lkb1 knock out' vs 'wild type'
Camp DOWN in 'Lkb1 knock out' vs 'wild type'
Col6a3 DOWN in 'Lkb1 knock out' vs 'wild type'
Slfn4 DOWN in 'Lkb1 knock out' vs 'wild type'
Npr2 DOWN in 'Lkb1 knock out' vs 'wild type'
Serpine2 UP in 'Lkb1 knock out' vs 'wild type'
Ngp DOWN in 'Lkb1 knock out' vs 'wild type'
Ifitm6 DOWN in 'Lkb1 knock out' vs 'wild type'
Mmp9 DOWN in 'Lkb1 knock out' vs 'wild type'
Spsb4 UP in 'Lkb1 knock out' vs 'wild type'
Ltf DOWN in 'Lkb1 knock out' vs 'wild type'
(Sample) cell type/LSK (Lin-, Sca-1+, c-Kit+) cells
Inadl DOWN in 'Lkb1 knock out' vs 'wild type'
Lgals3 DOWN in 'Lkb1 knock out' vs 'wild type'
Analysis of 'Lkb1 knock out' vs 'wild type'
Lgals1 UP in 'Lkb1 knock out' vs 'wild type'
Chdh UP in 'Lkb1 knock out' vs 'wild type'
Fpr2 DOWN in 'Lkb1 knock out' vs 'wild type'
Ccl6 DOWN in 'Lkb1 knock out' vs 'wild type'
Anxa3 UP in 'Lkb1 knock out' vs 'wild type'
Ermap DOWN in 'Lkb1 knock out' vs 'wild type'
Sumf1 UP in 'Lkb1 knock out' vs 'wild type'
Ly86 UP in 'Lkb1 knock out' vs 'wild type'
Pou2af1 DOWN in 'Lkb1 knock out' vs 'wild type'
Lcn2 DOWN in 'Lkb1 knock out' vs 'wild type'
Cdkn1a UP in 'Lkb1 knock out' vs 'wild type'
Nrp1 UP in 'Lkb1 knock out' vs 'wild type'
Cnn3 UP in 'Lkb1 knock out' vs 'wild type'
Car1 DOWN in 'Lkb1 knock out' vs 'wild type'
H2-Aa DOWN in 'Lkb1 knock out' vs 'wild type'
Chil1 DOWN in 'Lkb1 knock out' vs 'wild type'
LKB1 encodes a Ser/Thr kinase and acts as an evolutionarily conserved sensor of cellular energy status in eukaryotic cells. LKB1 functions as the major upstream kinase to phosphorylate AMPK and 12 other AMPK-related kinases, which is required for their activation in many cellular contexts. Once activated, AMPK and AMPK-related kinases phosphorylate a diverse array of downstream effectors to switch on ATP-generating catabolic processes and switch off ATP-consuming anabolic processes, thus restoring energy balance during periods of energetic stress. To study the role and mechanisms of Lkb1 in the regulation of hematopoietic stem cell (HSC) biology, we performed transcriptome analysis of sorted LSK (Lin-, Sca-1+, c-Kit+) cells from Lkb1 WT and KO bone marrows at 1 day post-completing tamoxifen injection (DPI). To identify more proximal molecular effects, we chose 1 DPI due to the modest phenotypes in Lkb1 KO mice, yet documentation of efficient Lkb1 deletion in LSK cells at this very early time point. We treated Lkb1 L/L rosa26CreERT2 and Lkb1 L/L mice (C57BL/Ka-CD45.2:Thy-1.1 background) with Tamoxifen for 5 days to somatically delete Lkb1 in adult mice, and generated Lkb1 WT and KO mice. At 1 DPI, we prepared single-cell suspensions from bone marrow (from femoral and tibial bones), and stained and sorted LSK populations using FACSAria (Becton Dickinson, Mountain View, CA). The RNA was extracted from sorted LSK cells, amplified and subjected to gene profiling. The samples include 3 Lkb1 WT (Lkb1 WT 5-7) and 4 Lkb1 KO (Lkb1 KO 4-7) replicates.
Fscn1 DOWN in 'Lkb1 knock out' vs 'wild type'
Cd177 DOWN in 'Lkb1 knock out' vs 'wild type'
Ryr3 DOWN in 'Lkb1 knock out' vs 'wild type'
S100a9 DOWN in 'Lkb1 knock out' vs 'wild type'
Dmkn UP in 'Lkb1 knock out' vs 'wild type'
Ceacam10 DOWN in 'Lkb1 knock out' vs 'wild type'
Sdc1 DOWN in 'Lkb1 knock out' vs 'wild type'