HDT4 UP in 'apum23 mutant' vs 'wild type'
CYC1 UP in 'apum23 mutant' vs 'wild type'
AT4G28450 UP in 'apum23 mutant' vs 'wild type'
AT1G13160 UP in 'apum23 mutant' vs 'wild type'
ATPAP29 DOWN in 'apum23 mutant' vs 'wild type'
RPB5D DOWN in 'apum23 mutant' vs 'wild type'
AT5G15550 UP in 'apum23 mutant' vs 'wild type'
AT1G72230 DOWN in 'apum23 mutant' vs 'wild type'
AT2G43780 UP in 'apum23 mutant' vs 'wild type'
ATCCS DOWN in 'apum23 mutant' vs 'wild type'
PNP2 UP in 'apum23 mutant' vs 'wild type'
AT1G17665 UP in 'apum23 mutant' vs 'wild type'
AT3G12860 UP in 'apum23 mutant' vs 'wild type'
IRT3 UP in 'apum23 mutant' vs 'wild type'
AT3G23880 DOWN in 'apum23 mutant' vs 'wild type'
WRKY25 UP in 'apum23 mutant' vs 'wild type'
AT2G18520 UP in 'apum23 mutant' vs 'wild type'
AT4G25730 UP in 'apum23 mutant' vs 'wild type'
RPL19B UP in 'apum23 mutant' vs 'wild type'
AT4G19430 DOWN in 'apum23 mutant' vs 'wild type'
AT5G57280 UP in 'apum23 mutant' vs 'wild type'
CRF6 UP in 'apum23 mutant' vs 'wild type'
AT5G59330 UP in 'apum23 mutant' vs 'wild type'
XTR2 UP in 'apum23 mutant' vs 'wild type'
AT1G13470 UP in 'apum23 mutant' vs 'wild type'
HLL UP in 'apum23 mutant' vs 'wild type'
AT5G14050 UP in 'apum23 mutant' vs 'wild type'
AT5G50450 UP in 'apum23 mutant' vs 'wild type'
PLP2 UP in 'apum23 mutant' vs 'wild type'
AT4G04940 UP in 'apum23 mutant' vs 'wild type'
AT2G15020 DOWN in 'apum23 mutant' vs 'wild type'
AT3G24780 UP in 'apum23 mutant' vs 'wild type'
AT1G69070 UP in 'apum23 mutant' vs 'wild type'
AT5G62440 UP in 'apum23 mutant' vs 'wild type'
AT2G40780 UP in 'apum23 mutant' vs 'wild type'
NRPA2 UP in 'apum23 mutant' vs 'wild type'
EIF3G2 UP in 'apum23 mutant' vs 'wild type'
TOM7-2 UP in 'apum23 mutant' vs 'wild type'
AT3G14700 UP in 'apum23 mutant' vs 'wild type'
AT2G05540 DOWN in 'apum23 mutant' vs 'wild type'
FKBP16-2 DOWN in 'apum23 mutant' vs 'wild type'
emb2742 UP in 'apum23 mutant' vs 'wild type'
ATCP1 UP in 'apum23 mutant' vs 'wild type'
TRFL3 UP in 'apum23 mutant' vs 'wild type'
AT5G20160 UP in 'apum23 mutant' vs 'wild type'
ECA2 UP in 'apum23 mutant' vs 'wild type'
REM21 DOWN in 'apum23 mutant' vs 'wild type'
AT5G46710 UP in 'apum23 mutant' vs 'wild type'
AT2G47780 UP in 'apum23 mutant' vs 'wild type'
AT1G56720 DOWN in 'apum23 mutant' vs 'wild type'
AT3G19850 DOWN in 'apum23 mutant' vs 'wild type'
AT1G30240 UP in 'apum23 mutant' vs 'wild type'
AT3G01160 UP in 'apum23 mutant' vs 'wild type'
ATASE UP in 'apum23 mutant' vs 'wild type'
SEN1 DOWN in 'apum23 mutant' vs 'wild type'
AT2G03130 UP in 'apum23 mutant' vs 'wild type'
AT1G12080 DOWN in 'apum23 mutant' vs 'wild type'
AT1G75670 UP in 'apum23 mutant' vs 'wild type'
PQL1 DOWN in 'apum23 mutant' vs 'wild type'
BXL2 DOWN in 'apum23 mutant' vs 'wild type'
AT5G49170 DOWN in 'apum23 mutant' vs 'wild type'
XTR7 DOWN in 'apum23 mutant' vs 'wild type'
AT2G47680 UP in 'apum23 mutant' vs 'wild type'
AGL19 UP in 'apum23 mutant' vs 'wild type'
AT3G15720 UP in 'apum23 mutant' vs 'wild type'
AT1G30160 UP in 'apum23 mutant' vs 'wild type'
AT4G21140 UP in 'apum23 mutant' vs 'wild type'
WRKY38 UP in 'apum23 mutant' vs 'wild type'
AT2G21640 UP in 'apum23 mutant' vs 'wild type'
RPL26B UP in 'apum23 mutant' vs 'wild type'
ATPRMT3 UP in 'apum23 mutant' vs 'wild type'
JAL23 DOWN in 'apum23 mutant' vs 'wild type'
COL9 UP in 'apum23 mutant' vs 'wild type'
ATGSTF12 UP in 'apum23 mutant' vs 'wild type'
HD2A UP in 'apum23 mutant' vs 'wild type'
AT1G03660 UP in 'apum23 mutant' vs 'wild type'
AT1G10640 DOWN in 'apum23 mutant' vs 'wild type'
AT3G58660 UP in 'apum23 mutant' vs 'wild type'
ATDI21 UP in 'apum23 mutant' vs 'wild type'
CYP78A8 DOWN in 'apum23 mutant' vs 'wild type'
AT1G10050 UP in 'apum23 mutant' vs 'wild type'
RRP41 UP in 'apum23 mutant' vs 'wild type'
ATNAF1 UP in 'apum23 mutant' vs 'wild type'
RPS27AA UP in 'apum23 mutant' vs 'wild type'
AT3G16310 UP in 'apum23 mutant' vs 'wild type'
DTXL1 UP in 'apum23 mutant' vs 'wild type'
EDA18 UP in 'apum23 mutant' vs 'wild type'
COL2 DOWN in 'apum23 mutant' vs 'wild type'
AT4G01700 UP in 'apum23 mutant' vs 'wild type'
AT3G03920 UP in 'apum23 mutant' vs 'wild type'
AT2G28600 UP in 'apum23 mutant' vs 'wild type'
POLD4 UP in 'apum23 mutant' vs 'wild type'
AT3G46490 DOWN in 'apum23 mutant' vs 'wild type'
WRKY53 UP in 'apum23 mutant' vs 'wild type'
AT3G07050 UP in 'apum23 mutant' vs 'wild type'
AT1G67120 UP in 'apum23 mutant' vs 'wild type'
CYP71B22 DOWN in 'apum23 mutant' vs 'wild type'
AT3G15080 UP in 'apum23 mutant' vs 'wild type'
AT3G11964 UP in 'apum23 mutant' vs 'wild type'
AT1G79150 UP in 'apum23 mutant' vs 'wild type'
AT4G23540 UP in 'apum23 mutant' vs 'wild type'
AT1G06720 UP in 'apum23 mutant' vs 'wild type'
TSO2 UP in 'apum23 mutant' vs 'wild type'
PMAT1 UP in 'apum23 mutant' vs 'wild type'
AT3G21540 UP in 'apum23 mutant' vs 'wild type'
CYP71A13 UP in 'apum23 mutant' vs 'wild type'
AT3G57000 UP in 'apum23 mutant' vs 'wild type'
GRXC13 DOWN in 'apum23 mutant' vs 'wild type'
ATMRU1 UP in 'apum23 mutant' vs 'wild type'
UBC17 DOWN in 'apum23 mutant' vs 'wild type'
AT2G20870 DOWN in 'apum23 mutant' vs 'wild type'
PAP1 UP in 'apum23 mutant' vs 'wild type'
anac053 UP in 'apum23 mutant' vs 'wild type'
AT2G41120 DOWN in 'apum23 mutant' vs 'wild type'
AT4G30990 UP in 'apum23 mutant' vs 'wild type'
AT3G26730 DOWN in 'apum23 mutant' vs 'wild type'
AT3G45730 UP in 'apum23 mutant' vs 'wild type'
WRKY57 UP in 'apum23 mutant' vs 'wild type'
AT2G45460 UP in 'apum23 mutant' vs 'wild type'
FMO1 UP in 'apum23 mutant' vs 'wild type'
ATPMEPCRB UP in 'apum23 mutant' vs 'wild type'
AOX1C UP in 'apum23 mutant' vs 'wild type'
AT1G24095 UP in 'apum23 mutant' vs 'wild type'
AT4G19130 UP in 'apum23 mutant' vs 'wild type'
FIB2 UP in 'apum23 mutant' vs 'wild type'
AT3G10530 UP in 'apum23 mutant' vs 'wild type'
NOP56 UP in 'apum23 mutant' vs 'wild type'
UGT76F2 DOWN in 'apum23 mutant' vs 'wild type'
AT1G49150 UP in 'apum23 mutant' vs 'wild type'
ATCSLA15 DOWN in 'apum23 mutant' vs 'wild type'
BG3 UP in 'apum23 mutant' vs 'wild type'
AT1G21520 UP in 'apum23 mutant' vs 'wild type'
NAP57 UP in 'apum23 mutant' vs 'wild type'
RCE2 UP in 'apum23 mutant' vs 'wild type'
AT4G05370 UP in 'apum23 mutant' vs 'wild type'
AT4G04180 UP in 'apum23 mutant' vs 'wild type'
AT1G17960 UP in 'apum23 mutant' vs 'wild type'
COPT2 UP in 'apum23 mutant' vs 'wild type'
AT5G45310 DOWN in 'apum23 mutant' vs 'wild type'
RPL27A UP in 'apum23 mutant' vs 'wild type'
AT2G19385 UP in 'apum23 mutant' vs 'wild type'
CHR34 UP in 'apum23 mutant' vs 'wild type'
AT3G13230 UP in 'apum23 mutant' vs 'wild type'
anac103 UP in 'apum23 mutant' vs 'wild type'
SWA1 UP in 'apum23 mutant' vs 'wild type'
AT2G18220 UP in 'apum23 mutant' vs 'wild type'
AT3G60290 DOWN in 'apum23 mutant' vs 'wild type'
UGT74E2 UP in 'apum23 mutant' vs 'wild type'
AT3G55920 UP in 'apum23 mutant' vs 'wild type'
ELF4 UP in 'apum23 mutant' vs 'wild type'
IMPDH UP in 'apum23 mutant' vs 'wild type'
RH17 UP in 'apum23 mutant' vs 'wild type'
AT4G27010 UP in 'apum23 mutant' vs 'wild type'
NDB2 UP in 'apum23 mutant' vs 'wild type'
AT1G50920 UP in 'apum23 mutant' vs 'wild type'
AHP2 UP in 'apum23 mutant' vs 'wild type'
STZ UP in 'apum23 mutant' vs 'wild type'
AT3G06530 UP in 'apum23 mutant' vs 'wild type'
AT2G32280 UP in 'apum23 mutant' vs 'wild type'
AT3G13940 UP in 'apum23 mutant' vs 'wild type'
CSD1 DOWN in 'apum23 mutant' vs 'wild type'
WRKY47 UP in 'apum23 mutant' vs 'wild type'
APUM24 UP in 'apum23 mutant' vs 'wild type'
CHI UP in 'apum23 mutant' vs 'wild type'
ROC2 UP in 'apum23 mutant' vs 'wild type'
AT3G15357 UP in 'apum23 mutant' vs 'wild type'
AT3G53210 UP in 'apum23 mutant' vs 'wild type'
AT1G23280 UP in 'apum23 mutant' vs 'wild type'
AT2G35570 UP in 'apum23 mutant' vs 'wild type'
GMI1 UP in 'apum23 mutant' vs 'wild type'
APP UP in 'apum23 mutant' vs 'wild type'
RPL35B UP in 'apum23 mutant' vs 'wild type'
AT1G62730 UP in 'apum23 mutant' vs 'wild type'
ATPGP1 UP in 'apum23 mutant' vs 'wild type'
AT4G02220 UP in 'apum23 mutant' vs 'wild type'
PANB2 UP in 'apum23 mutant' vs 'wild type'
GRXS13 UP in 'apum23 mutant' vs 'wild type'
PRMT10 UP in 'apum23 mutant' vs 'wild type'
KCS17 UP in 'apum23 mutant' vs 'wild type'
Analysis of 'apum23 mutant' vs 'wild type'
AT3G12510 UP in 'apum23 mutant' vs 'wild type'
ATTIM17-1 UP in 'apum23 mutant' vs 'wild type'
ATBRCA1 UP in 'apum23 mutant' vs 'wild type'
AT1G20070 DOWN in 'apum23 mutant' vs 'wild type'
AT2G43590 UP in 'apum23 mutant' vs 'wild type'
ATCSLG3 UP in 'apum23 mutant' vs 'wild type'
RPL37A UP in 'apum23 mutant' vs 'wild type'
AT3G59820 UP in 'apum23 mutant' vs 'wild type'
APUM23 DOWN in 'apum23 mutant' vs 'wild type'
AT2G40360 UP in 'apum23 mutant' vs 'wild type'
AT1G12250 DOWN in 'apum23 mutant' vs 'wild type'
CER1 UP in 'apum23 mutant' vs 'wild type'
PWP2 UP in 'apum23 mutant' vs 'wild type'
PYRB UP in 'apum23 mutant' vs 'wild type'
AT2G39650 UP in 'apum23 mutant' vs 'wild type'
BT2 UP in 'apum23 mutant' vs 'wild type'
TIF3C2 UP in 'apum23 mutant' vs 'wild type'
ATAAH DOWN in 'apum23 mutant' vs 'wild type'
AT3G02400 UP in 'apum23 mutant' vs 'wild type'
DTXL2 UP in 'apum23 mutant' vs 'wild type'
EMB2777 UP in 'apum23 mutant' vs 'wild type'
LTP2 UP in 'apum23 mutant' vs 'wild type'
GPT2 UP in 'apum23 mutant' vs 'wild type'
TRM11 UP in 'apum23 mutant' vs 'wild type'
MDR4 UP in 'apum23 mutant' vs 'wild type'
DREB26 UP in 'apum23 mutant' vs 'wild type'
COL7 DOWN in 'apum23 mutant' vs 'wild type'
chr31 UP in 'apum23 mutant' vs 'wild type'
CSD2 DOWN in 'apum23 mutant' vs 'wild type'
AT1G02870 UP in 'apum23 mutant' vs 'wild type'
PNP-A UP in 'apum23 mutant' vs 'wild type'
AT1G61640 UP in 'apum23 mutant' vs 'wild type'
ATCOX17 UP in 'apum23 mutant' vs 'wild type'
ATPK1 UP in 'apum23 mutant' vs 'wild type'
AT5G61340 DOWN in 'apum23 mutant' vs 'wild type'
ATNHX6 UP in 'apum23 mutant' vs 'wild type'
ATTI2 DOWN in 'apum23 mutant' vs 'wild type'
EDA25 UP in 'apum23 mutant' vs 'wild type'
AT2G34260 UP in 'apum23 mutant' vs 'wild type'
RPP0A UP in 'apum23 mutant' vs 'wild type'
AT4G22960 UP in 'apum23 mutant' vs 'wild type'
RH51 UP in 'apum23 mutant' vs 'wild type'
ZIP5 UP in 'apum23 mutant' vs 'wild type'
XRI UP in 'apum23 mutant' vs 'wild type'
COR414-TM1 DOWN in 'apum23 mutant' vs 'wild type'
AT1G66100 DOWN in 'apum23 mutant' vs 'wild type'
ATMES7 DOWN in 'apum23 mutant' vs 'wild type'
TRXF2 DOWN in 'apum23 mutant' vs 'wild type'
IMP4 UP in 'apum23 mutant' vs 'wild type'
AT1G49050 UP in 'apum23 mutant' vs 'wild type'
RXF26 DOWN in 'apum23 mutant' vs 'wild type'
BETA-OHASE 2 DOWN in 'apum23 mutant' vs 'wild type'
ARAC7 UP in 'apum23 mutant' vs 'wild type'
AT1G04945 UP in 'apum23 mutant' vs 'wild type'
AT2G24255 UP in 'apum23 mutant' vs 'wild type'
RPP2C UP in 'apum23 mutant' vs 'wild type'
RPS2D UP in 'apum23 mutant' vs 'wild type'
FKBP53 UP in 'apum23 mutant' vs 'wild type'
AT1G20390 UP in 'apum23 mutant' vs 'wild type'
CYP82G1 UP in 'apum23 mutant' vs 'wild type'
DREB19 UP in 'apum23 mutant' vs 'wild type'
AT1G23000 UP in 'apum23 mutant' vs 'wild type'
AZF2 UP in 'apum23 mutant' vs 'wild type'
TBL6 DOWN in 'apum23 mutant' vs 'wild type'
YAO UP in 'apum23 mutant' vs 'wild type'
EDA14 UP in 'apum23 mutant' vs 'wild type'
AT1G07050 UP in 'apum23 mutant' vs 'wild type'
PARLL1 UP in 'apum23 mutant' vs 'wild type'
ACC2 UP in 'apum23 mutant' vs 'wild type'
AT3G54500 DOWN in 'apum23 mutant' vs 'wild type'
AT1G52930 UP in 'apum23 mutant' vs 'wild type'
AT3G10930 UP in 'apum23 mutant' vs 'wild type'
AT1G67760 UP in 'apum23 mutant' vs 'wild type'
AT5G03350 UP in 'apum23 mutant' vs 'wild type'
AT1G22550 DOWN in 'apum23 mutant' vs 'wild type'
NOV UP in 'apum23 mutant' vs 'wild type'
RPL7A UP in 'apum23 mutant' vs 'wild type'
AT2G18720 UP in 'apum23 mutant' vs 'wild type'
AT3G22660 UP in 'apum23 mutant' vs 'wild type'
ARF31 UP in 'apum23 mutant' vs 'wild type'
TAR2 DOWN in 'apum23 mutant' vs 'wild type'
AT2G18180 UP in 'apum23 mutant' vs 'wild type'
AT2G44860 UP in 'apum23 mutant' vs 'wild type'
RH57 UP in 'apum23 mutant' vs 'wild type'
TUB1 DOWN in 'apum23 mutant' vs 'wild type'
AT4G15770 UP in 'apum23 mutant' vs 'wild type'
CYP81F2 UP in 'apum23 mutant' vs 'wild type'
AT3G13150 UP in 'apum23 mutant' vs 'wild type'
AT4G38950 DOWN in 'apum23 mutant' vs 'wild type'
PGL5 DOWN in 'apum23 mutant' vs 'wild type'
SIB1 UP in 'apum23 mutant' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
TIM13 UP in 'apum23 mutant' vs 'wild type'
AT4G02400 UP in 'apum23 mutant' vs 'wild type'
AT3G61920 UP in 'apum23 mutant' vs 'wild type'
AT2G41730 UP in 'apum23 mutant' vs 'wild type'
AT1G03440 UP in 'apum23 mutant' vs 'wild type'
AT4G29270 DOWN in 'apum23 mutant' vs 'wild type'
AT2G18190 UP in 'apum23 mutant' vs 'wild type'
TRFL10 UP in 'apum23 mutant' vs 'wild type'
AT5G11460 UP in 'apum23 mutant' vs 'wild type'
AT3G52170 UP in 'apum23 mutant' vs 'wild type'
AT3G46370 DOWN in 'apum23 mutant' vs 'wild type'
RH34 UP in 'apum23 mutant' vs 'wild type'
WSD1 UP in 'apum23 mutant' vs 'wild type'
Gene expression analyses of three-week old soil grown wild-type (Col-3) and apum23-1 mutant. Expression profiling was conducted as an attempt to identify the genes differentially regulated in apum23-1 mutant This study was under taken to characterize a member of pumilio family, APUM23 in Arabidopsis. Pumilio, an RNA-binding protein (RBP) containing tandem repeat PUF domains, has been known to repress translational activity in early embryogenesis and polarized cells of non-plant species. Although Pumilio proteins have been characterized in many eukaryotes, their roles in plants are unknown. We detail the characterization of an Arabidopsis Pumilio gene, APUM23. APUM23 is constitutively expressed with higher level in metabolically active tissues, and upregulated in the presence of either glucose or sucrose. The T-DNA insertion mutants apum23-1 and apum23-2 showed slow growth with serrated and scrunched leaves, abnormal venation pattern, and distorted organization of the palisade parenchyma cells, a phenotype reminiscent of nucleolin and ribosomal protein gene mutants. Intracellular localization studies indicate that APUM23 predominantly localizes to the nucleolus. Based on the localization, rRNA processing was examined in apum23 mutant. In apum23, 35S pre-rRNA and unprocessed 18S and 5.8S poly(A) rRNAs were accumulated without affecting steady-state levels of mature rRNAs, indicating that APUM23 participates in the degradation of rRNA by-products. The apum23 mutant showed increased levels of 18S rRNA biogenesis-related U3 and U14 snoRNAs, and accumulated RNAs within nucleolus. Our results suggest that APUM23 plays an important role in plant development via rRNA processing and ribosome biogenesis. To identify the genes affected in apum23-1 as compared to wild-type (Col-3) under normal growth conditions using Arabidopsis ATH1 Genome arrays (Affymetrix). RNA samples from these plants were used to generate biotin labelled (cDNA) probes, which were then hybridized to the microarray. For data set generation, three independent samples were used, three from Col-3 and three from apum23-1 plants grown on soil for 21 days.
ATALN DOWN in 'apum23 mutant' vs 'wild type'
AT3G05600 DOWN in 'apum23 mutant' vs 'wild type'
AT1G31660 UP in 'apum23 mutant' vs 'wild type'
AT3G25940 UP in 'apum23 mutant' vs 'wild type'
ATRL5 DOWN in 'apum23 mutant' vs 'wild type'
tic20-IV UP in 'apum23 mutant' vs 'wild type'
PAD3 UP in 'apum23 mutant' vs 'wild type'
AT2G34357 UP in 'apum23 mutant' vs 'wild type'
QQS DOWN in 'apum23 mutant' vs 'wild type'
GRF11 DOWN in 'apum23 mutant' vs 'wild type'
ATPPT2 DOWN in 'apum23 mutant' vs 'wild type'
ARR15 DOWN in 'apum23 mutant' vs 'wild type'
AZF3 UP in 'apum23 mutant' vs 'wild type'
AT3G05170 UP in 'apum23 mutant' vs 'wild type'