Cyb5b UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Hsdl2 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Hmox1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Asns UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Car3 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Atf5 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Gas2 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Ces2a UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Egr1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Igf1 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp26a1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Shmt1 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Hsph1 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Dhcr7 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Pgd UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Rpgrip1 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Scd1 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Ethe1 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Chpt1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
As3mt DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
(Sample) strain/mixed C57BL/6 and 129/Sv
Cyp2c55 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
BC089597 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Cd36 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cldn3 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Dbp DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyb5b UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Saa1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Car3 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Acot1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Gadd45g DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Tcea3 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Gamt UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Atp1a1 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gstm1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Grn DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Hsd17b11 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp2b10 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Aqp8 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
(Sample) genotype/hypomorphic Cpr (Cpr low )
Nudt7 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Hmgcs1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gstm3 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Elovl5 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Hmgcs1 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Abhd1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Tgm2 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
(Factor value) GENOTYPE/hypomorphic Cpr (Cpr low )
Gsta2 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Cyp7b1 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Ethe1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Ccrn4l DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Aldh1a7 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Aqp8 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Sbk1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Apoa4 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Fgl1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Fabp2 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Papss2 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
4931406C07Rik UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Akr1d1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Ces1g UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Usp2 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Fabp2 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Rgs16 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Sdc4 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gdpd3 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Fasn UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
(Sample) genotype/Liver-Cpr-null deletion
Sqle UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Sds DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Msmo1 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
NADPH-cytochrome P450 reductase (CPR) is important for the functions of many enzymes, such as microsomal cytochrome P450 (P450) monooxygenases and heme oxygenases. Two mouse models with deficient CPR expression in adults were recently generated in this laboratory: liver-Cpr-null (with liver-specific Cpr deletion) (Gu et al., J. Biol. Chem., 278, 25895-25901, 2003) and Cpr-low (with reduced CPR expression in all organs examined) (Wu et al. J. Pharmacol. Expt. Ther. 312, 35-43, 2005). The phenotypes included a reduced serum cholesterol level and an induction of hepatic P450 in both models, and hepatomegaly and fatty liver in the liver-Cpr-null mouse alone. Our aim was to identify hepatic gene-expression changes related to these phenotypes. Cpr-lox mice, which have normal CPR expression (Wu et al., Genesis, 36, 177-181, 2003.), were used as the control in microarray analysis. A detailed analysis of the gene-expression changes in lipid metabolism and transport pathways revealed potential mechanisms, such as an increased activation of constitutive androstane receptor (CAR) and a decreased activation of peroxisomal proliferators activated receptor alpha (PPARÃÂ¯ÃÂÃÂ¡) by precursors of cholesterol biosynthesis, that underlie common changes (e.g., induction of multiple P450s and inhibition of genes for fatty acids metabolism) in response to CPR-loss in the two mouse models. Moreover, we also uncovered model-specific gene-expression changes, such as the induction of a lipid translocase (CD36 antigen) and the suppression of carnitine O-palmitoyltransferase 1 (CPT1a) and acyl-CoA synthetase long-chain family member 1 (Acsl1), that are potentially responsible for the severe hepatic lipidosis observed in liver-Cpr-null, but not Cpr-low mice.
Hsd3b5 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp51 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
(Factor value) GENOTYPE/Liver-Cpr-null deletion
Nr0b2 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Bdh1 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp4a10 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Gstm6 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Idi1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Rab30 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Tubb2a UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp2b10 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Entpd5 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Tmem176a UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gstm3 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp7a1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Asic5 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gsta3 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Gadd45b UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp51 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Analysis of 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Fgf1 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Analysis of 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Vnn1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Hsd17b10 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Acss2 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Csad UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Slco1a4 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gck UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Ahr UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Lgals1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Idi1 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Peg3 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Trp53inp1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Rhbg UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Dclre1a UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp4a14 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
AW112010 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Anxa5 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Antxr2 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp17a1 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Prnp UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Mtap DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Cyp2c55 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Gamt UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Pnpla7 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Retsat DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Ehhadh DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Serpina3k DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Lpl UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Sqle UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Lgalsl UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Ugt2b34 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Mafb DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gstm2 UP in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Gstm6 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Sult1d1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Msmo1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Ugdh UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Apoa4 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp7b1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Ehhadh DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Hsd3b2 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Cyp2c39 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Mt2 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Rab30 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Hsph1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Cyp17a1 DOWN in 'hypomorphic Cpr (Cpr low)' vs 'Cpr-lox control'
Scd1 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Dhcr24 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gsta2 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gstm2 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Gstt3 UP in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'
Phlda1 DOWN in 'Liver-Cpr-null deletion' vs 'Cpr-lox control'