Eh UP in 'CrebA null mutant' vs 'wild type'
membrin DOWN in 'CrebA null mutant' vs 'wild type'
CG17738 UP in 'CrebA null mutant' vs 'wild type'
CG32276 DOWN in 'CrebA null mutant' vs 'wild type'
CG6106 UP in 'CrebA null mutant' vs 'wild type'
Obp18a DOWN in 'CrebA null mutant' vs 'wild type'
CG18814 UP in 'CrebA null mutant' vs 'wild type'
GstE9 UP in 'CrebA null mutant' vs 'wild type'
CG8620 UP in 'CrebA null mutant' vs 'wild type'
dro2 UP in 'CrebA null mutant' vs 'wild type'
Cpr78Cb DOWN in 'CrebA null mutant' vs 'wild type'
CG42822 DOWN in 'CrebA null mutant' vs 'wild type'
CG7432 UP in 'CrebA null mutant' vs 'wild type'
CG14317 UP in 'CrebA null mutant' vs 'wild type'
(Sample) organism/Drosophila melanogaster
sel DOWN in 'CrebA null mutant' vs 'wild type'
IM3 UP in 'CrebA null mutant' vs 'wild type'
CG14302 UP in 'CrebA null mutant' vs 'wild type'
bai DOWN in 'CrebA null mutant' vs 'wild type'
CG10086 UP in 'CrebA null mutant' vs 'wild type'
GstE2 UP in 'CrebA null mutant' vs 'wild type'
alphaTry UP in 'CrebA null mutant' vs 'wild type'
Cyp9b2 UP in 'CrebA null mutant' vs 'wild type'
CG8628 UP in 'CrebA null mutant' vs 'wild type'
MtnD UP in 'CrebA null mutant' vs 'wild type'
CG16959 UP in 'CrebA null mutant' vs 'wild type'
SrpRbeta DOWN in 'CrebA null mutant' vs 'wild type'
Dh31-R1 UP in 'CrebA null mutant' vs 'wild type'
SsRbeta DOWN in 'CrebA null mutant' vs 'wild type'
Victoria DOWN in 'CrebA null mutant' vs 'wild type'
KaiRIA DOWN in 'CrebA null mutant' vs 'wild type'
CG33474 UP in 'CrebA null mutant' vs 'wild type'
Sec61gamma DOWN in 'CrebA null mutant' vs 'wild type'
CG9084 UP in 'CrebA null mutant' vs 'wild type'
CG5397 UP in 'CrebA null mutant' vs 'wild type'
CG11825 UP in 'CrebA null mutant' vs 'wild type'
RhoGAP71E DOWN in 'CrebA null mutant' vs 'wild type'
CG15043 UP in 'CrebA null mutant' vs 'wild type'
Analysis of 'CrebA null mutant' vs 'wild type'
GstE1 UP in 'CrebA null mutant' vs 'wild type'
pip DOWN in 'CrebA null mutant' vs 'wild type'
CG10911 UP in 'CrebA null mutant' vs 'wild type'
Cpr76Bc UP in 'CrebA null mutant' vs 'wild type'
CG17669 UP in 'CrebA null mutant' vs 'wild type'
CG34301 UP in 'CrebA null mutant' vs 'wild type'
CG18343 DOWN in 'CrebA null mutant' vs 'wild type'
CG18367 UP in 'CrebA null mutant' vs 'wild type'
CG32302 DOWN in 'CrebA null mutant' vs 'wild type'
CG34449 DOWN in 'CrebA null mutant' vs 'wild type'
Ddc UP in 'CrebA null mutant' vs 'wild type'
loj DOWN in 'CrebA null mutant' vs 'wild type'
CG5656 DOWN in 'CrebA null mutant' vs 'wild type'
CG8249 DOWN in 'CrebA null mutant' vs 'wild type'
CG10799 UP in 'CrebA null mutant' vs 'wild type'
C901 DOWN in 'CrebA null mutant' vs 'wild type'
CG13075 UP in 'CrebA null mutant' vs 'wild type'
CG34166 UP in 'CrebA null mutant' vs 'wild type'
Edem1 DOWN in 'CrebA null mutant' vs 'wild type'
CG8157 UP in 'CrebA null mutant' vs 'wild type'
mex1 DOWN in 'CrebA null mutant' vs 'wild type'
LysX DOWN in 'CrebA null mutant' vs 'wild type'
mle DOWN in 'CrebA null mutant' vs 'wild type'
Grasp65 DOWN in 'CrebA null mutant' vs 'wild type'
CG9989 DOWN in 'CrebA null mutant' vs 'wild type'
CG8960 UP in 'CrebA null mutant' vs 'wild type'
CG16799 DOWN in 'CrebA null mutant' vs 'wild type'
msta UP in 'CrebA null mutant' vs 'wild type'
CG6067 UP in 'CrebA null mutant' vs 'wild type'
CG1749 DOWN in 'CrebA null mutant' vs 'wild type'
PGRP-LA UP in 'CrebA null mutant' vs 'wild type'
Mtp DOWN in 'CrebA null mutant' vs 'wild type'
yellow-e2 DOWN in 'CrebA null mutant' vs 'wild type'
pnt DOWN in 'CrebA null mutant' vs 'wild type'
CG13116 UP in 'CrebA null mutant' vs 'wild type'
Lcp3 DOWN in 'CrebA null mutant' vs 'wild type'
Drs UP in 'CrebA null mutant' vs 'wild type'
CG10912 UP in 'CrebA null mutant' vs 'wild type'
CG13658 UP in 'CrebA null mutant' vs 'wild type'
Odc1 UP in 'CrebA null mutant' vs 'wild type'
CG31706 UP in 'CrebA null mutant' vs 'wild type'
CG15068 UP in 'CrebA null mutant' vs 'wild type'
Spn28Da UP in 'CrebA null mutant' vs 'wild type'
CG7290 UP in 'CrebA null mutant' vs 'wild type'
CG11034 UP in 'CrebA null mutant' vs 'wild type'
CG15743 DOWN in 'CrebA null mutant' vs 'wild type'
CG1673 UP in 'CrebA null mutant' vs 'wild type'
CG1077 DOWN in 'CrebA null mutant' vs 'wild type'
Syt12 UP in 'CrebA null mutant' vs 'wild type'
CG14866 UP in 'CrebA null mutant' vs 'wild type'
Rh50 UP in 'CrebA null mutant' vs 'wild type'
CG13868 UP in 'CrebA null mutant' vs 'wild type'
CG33169 DOWN in 'CrebA null mutant' vs 'wild type'
CG12384 DOWN in 'CrebA null mutant' vs 'wild type'
CG11550 UP in 'CrebA null mutant' vs 'wild type'
CG18661 DOWN in 'CrebA null mutant' vs 'wild type'
CG13876 UP in 'CrebA null mutant' vs 'wild type'
igl UP in 'CrebA null mutant' vs 'wild type'
RFeSP UP in 'CrebA null mutant' vs 'wild type'
CG1698 UP in 'CrebA null mutant' vs 'wild type'
CG43386 UP in 'CrebA null mutant' vs 'wild type'
Eip63F-1 UP in 'CrebA null mutant' vs 'wild type'
CG42335 DOWN in 'CrebA null mutant' vs 'wild type'
CG31235 UP in 'CrebA null mutant' vs 'wild type'
PGRP-LB UP in 'CrebA null mutant' vs 'wild type'
CG34005 UP in 'CrebA null mutant' vs 'wild type'
CG12540 UP in 'CrebA null mutant' vs 'wild type'
CG14850 DOWN in 'CrebA null mutant' vs 'wild type'
CG7433 UP in 'CrebA null mutant' vs 'wild type'
CG11905 DOWN in 'CrebA null mutant' vs 'wild type'
CG31789 UP in 'CrebA null mutant' vs 'wild type'
CG11370 UP in 'CrebA null mutant' vs 'wild type'
Prosap DOWN in 'CrebA null mutant' vs 'wild type'
CG7300 DOWN in 'CrebA null mutant' vs 'wild type'
Muc18B UP in 'CrebA null mutant' vs 'wild type'
CG43164 DOWN in 'CrebA null mutant' vs 'wild type'
CG10365 UP in 'CrebA null mutant' vs 'wild type'
mthl8 DOWN in 'CrebA null mutant' vs 'wild type'
CG30272 DOWN in 'CrebA null mutant' vs 'wild type'
CG6912 DOWN in 'CrebA null mutant' vs 'wild type'
CG16926 UP in 'CrebA null mutant' vs 'wild type'
CrebA DOWN in 'CrebA null mutant' vs 'wild type'
CG14105 DOWN in 'CrebA null mutant' vs 'wild type'
Spn5 UP in 'CrebA null mutant' vs 'wild type'
CG43117 DOWN in 'CrebA null mutant' vs 'wild type'
Npc2h UP in 'CrebA null mutant' vs 'wild type'
CG1468 UP in 'CrebA null mutant' vs 'wild type'
Arc1 UP in 'CrebA null mutant' vs 'wild type'
Bace UP in 'CrebA null mutant' vs 'wild type'
CG14453 DOWN in 'CrebA null mutant' vs 'wild type'
ftz-f1 UP in 'CrebA null mutant' vs 'wild type'
Jon44E DOWN in 'CrebA null mutant' vs 'wild type'
alphaCop DOWN in 'CrebA null mutant' vs 'wild type'
CG8100 DOWN in 'CrebA null mutant' vs 'wild type'
mst UP in 'CrebA null mutant' vs 'wild type'
scb UP in 'CrebA null mutant' vs 'wild type'
CG6188 UP in 'CrebA null mutant' vs 'wild type'
CG15362 DOWN in 'CrebA null mutant' vs 'wild type'
CG13659 UP in 'CrebA null mutant' vs 'wild type'
CG6084 UP in 'CrebA null mutant' vs 'wild type'
ergic53 DOWN in 'CrebA null mutant' vs 'wild type'
CG34437 UP in 'CrebA null mutant' vs 'wild type'
CG14636 UP in 'CrebA null mutant' vs 'wild type'
w DOWN in 'CrebA null mutant' vs 'wild type'
CG14756 DOWN in 'CrebA null mutant' vs 'wild type'
CG15394 UP in 'CrebA null mutant' vs 'wild type'
CG32483 DOWN in 'CrebA null mutant' vs 'wild type'
Cpr67Fa2 DOWN in 'CrebA null mutant' vs 'wild type'
GstD10 UP in 'CrebA null mutant' vs 'wild type'
CG4293 DOWN in 'CrebA null mutant' vs 'wild type'
CG6403 UP in 'CrebA null mutant' vs 'wild type'
CG31313 DOWN in 'CrebA null mutant' vs 'wild type'
CG16713 UP in 'CrebA null mutant' vs 'wild type'
CG14257 UP in 'CrebA null mutant' vs 'wild type'
CG9737 UP in 'CrebA null mutant' vs 'wild type'
CG15530 UP in 'CrebA null mutant' vs 'wild type'
S-Lap1 DOWN in 'CrebA null mutant' vs 'wild type'
ImpL1 DOWN in 'CrebA null mutant' vs 'wild type'
Fbxl4 UP in 'CrebA null mutant' vs 'wild type'
zetaCOP DOWN in 'CrebA null mutant' vs 'wild type'
CG10910 UP in 'CrebA null mutant' vs 'wild type'
Spase25 DOWN in 'CrebA null mutant' vs 'wild type'
CG13324 UP in 'CrebA null mutant' vs 'wild type'
Tapdelta DOWN in 'CrebA null mutant' vs 'wild type'
Cpr64Ac UP in 'CrebA null mutant' vs 'wild type'
Spp DOWN in 'CrebA null mutant' vs 'wild type'
CG8774 UP in 'CrebA null mutant' vs 'wild type'
CG17244 UP in 'CrebA null mutant' vs 'wild type'
CG17110 DOWN in 'CrebA null mutant' vs 'wild type'
Cyp49a1 UP in 'CrebA null mutant' vs 'wild type'
CG18136 UP in 'CrebA null mutant' vs 'wild type'
Jafrac2 DOWN in 'CrebA null mutant' vs 'wild type'
CG18547 UP in 'CrebA null mutant' vs 'wild type'
CG32281 DOWN in 'CrebA null mutant' vs 'wild type'
CG11131 UP in 'CrebA null mutant' vs 'wild type'
CG16898 UP in 'CrebA null mutant' vs 'wild type'
Dh31 UP in 'CrebA null mutant' vs 'wild type'
(Factor value) GENOTYPE/CrebA null mutant
Tsp42Ec UP in 'CrebA null mutant' vs 'wild type'
mod(mdg4) UP in 'CrebA null mutant' vs 'wild type'
CG17271 DOWN in 'CrebA null mutant' vs 'wild type'
CG7299 UP in 'CrebA null mutant' vs 'wild type'
CG12011 DOWN in 'CrebA null mutant' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
CG13813 UP in 'CrebA null mutant' vs 'wild type'
CG6738 UP in 'CrebA null mutant' vs 'wild type'
TRAM DOWN in 'CrebA null mutant' vs 'wild type'
CG18088 DOWN in 'CrebA null mutant' vs 'wild type'
CG2177 DOWN in 'CrebA null mutant' vs 'wild type'
CG18765 UP in 'CrebA null mutant' vs 'wild type'
CG7567 DOWN in 'CrebA null mutant' vs 'wild type'
CG5697 UP in 'CrebA null mutant' vs 'wild type'
CAH2 UP in 'CrebA null mutant' vs 'wild type'
CG12858 UP in 'CrebA null mutant' vs 'wild type'
CG7992 UP in 'CrebA null mutant' vs 'wild type'
CG8160 UP in 'CrebA null mutant' vs 'wild type'
CG14957 UP in 'CrebA null mutant' vs 'wild type'
path DOWN in 'CrebA null mutant' vs 'wild type'
CG10035 UP in 'CrebA null mutant' vs 'wild type'
CG18641 UP in 'CrebA null mutant' vs 'wild type'
CG14183 UP in 'CrebA null mutant' vs 'wild type'
Rala UP in 'CrebA null mutant' vs 'wild type'
Sry-alpha UP in 'CrebA null mutant' vs 'wild type'
nimB5 UP in 'CrebA null mutant' vs 'wild type'
v UP in 'CrebA null mutant' vs 'wild type'
Esyt2 UP in 'CrebA null mutant' vs 'wild type'
CG42565 UP in 'CrebA null mutant' vs 'wild type'
CG34025 UP in 'CrebA null mutant' vs 'wild type'
CG1969 DOWN in 'CrebA null mutant' vs 'wild type'
CG9186 DOWN in 'CrebA null mutant' vs 'wild type'
CG32073 DOWN in 'CrebA null mutant' vs 'wild type'
kek5 UP in 'CrebA null mutant' vs 'wild type'
CR31781 UP in 'CrebA null mutant' vs 'wild type'
CG4783 UP in 'CrebA null mutant' vs 'wild type'
CG32368 DOWN in 'CrebA null mutant' vs 'wild type'
CG5768 DOWN in 'CrebA null mutant' vs 'wild type'
CG7945 DOWN in 'CrebA null mutant' vs 'wild type'
CG7201 UP in 'CrebA null mutant' vs 'wild type'
CG13738 DOWN in 'CrebA null mutant' vs 'wild type'
mthl5 DOWN in 'CrebA null mutant' vs 'wild type'
Csat DOWN in 'CrebA null mutant' vs 'wild type'
IM4 UP in 'CrebA null mutant' vs 'wild type'
SNCF UP in 'CrebA null mutant' vs 'wild type'
Ilp8 UP in 'CrebA null mutant' vs 'wild type'
IM23 UP in 'CrebA null mutant' vs 'wild type'
CG18585 DOWN in 'CrebA null mutant' vs 'wild type'
CG3835 UP in 'CrebA null mutant' vs 'wild type'
MtnA UP in 'CrebA null mutant' vs 'wild type'
CG2023 DOWN in 'CrebA null mutant' vs 'wild type'
Cyp310a1 DOWN in 'CrebA null mutant' vs 'wild type'
CG16743 UP in 'CrebA null mutant' vs 'wild type'
CG31676 UP in 'CrebA null mutant' vs 'wild type'
CG1418 DOWN in 'CrebA null mutant' vs 'wild type'
Fst UP in 'CrebA null mutant' vs 'wild type'
Lcp65Af DOWN in 'CrebA null mutant' vs 'wild type'
CHOp24 DOWN in 'CrebA null mutant' vs 'wild type'
obst-H DOWN in 'CrebA null mutant' vs 'wild type'
CG10116 DOWN in 'CrebA null mutant' vs 'wild type'
yellow-d UP in 'CrebA null mutant' vs 'wild type'
CG11659 UP in 'CrebA null mutant' vs 'wild type'
CG5402 DOWN in 'CrebA null mutant' vs 'wild type'
CG34224 DOWN in 'CrebA null mutant' vs 'wild type'
CCHa2 UP in 'CrebA null mutant' vs 'wild type'
alpha-Est2 UP in 'CrebA null mutant' vs 'wild type'
CG14022 UP in 'CrebA null mutant' vs 'wild type'
CG6503 UP in 'CrebA null mutant' vs 'wild type'
Abstract: Secretion occurs in all cells, with relatively low levels in most cells and extremely high levels in specialized secretory cells, such as those of the pancreas, salivary and mammary glands. Here, we report that the CrebA/Creb3-like family of bZip transcription factors functions to upregulate expression of both the general protein machinery required in all cells for secretion and of cell-type specific secreted proteins. Drosophila CrebA directly binds the enhancers of secretory pathway genes and is both necessary and sufficient to activate expression of every secretory pathway component gene examined thus far. Microarray profiling reveals that CrebA also upregulates expression of genes encoding cell type specific secreted components. Finally, we find that the human CrebA orthologues, Creb3L1 and Creb3L2, have the ability to upregulate the secretory pathway in non-secretory cell types. Goals: The goals of the microarray experiments were to identify additional targets of the CrebA transcription factor to learn the range of genes regulated by this transcription factor during embryogenesis. Previous work had indicated that CrebA upregulates the protein machinery of the early secretory pathway. Our new data now shows that in addition to the protein machinery, CrebA also upregulates genes encoding the protein cargo that is secreted from specialized secretory organs. RNA was isolated from stage 11-15 wild type Drosophila embryos and compared to RNA from CrebA null mutant embryos of the same age; all samples were hybridized to the Drosophila Genome 2.0 Affymetrix array. Three individual replicates were obtained for each sample.
CG31809 DOWN in 'CrebA null mutant' vs 'wild type'
CG5804 UP in 'CrebA null mutant' vs 'wild type'
Bet1 DOWN in 'CrebA null mutant' vs 'wild type'
CG9449 DOWN in 'CrebA null mutant' vs 'wild type'
Cpr47Eg DOWN in 'CrebA null mutant' vs 'wild type'
CG2812 DOWN in 'CrebA null mutant' vs 'wild type'
Mur18B UP in 'CrebA null mutant' vs 'wild type'
CG31674 UP in 'CrebA null mutant' vs 'wild type'
Nha1 UP in 'CrebA null mutant' vs 'wild type'
Tsp42En UP in 'CrebA null mutant' vs 'wild type'
Cpr47Eb UP in 'CrebA null mutant' vs 'wild type'
Bsg25A UP in 'CrebA null mutant' vs 'wild type'
Tsp42El UP in 'CrebA null mutant' vs 'wild type'
Spn DOWN in 'CrebA null mutant' vs 'wild type'
CG10514 DOWN in 'CrebA null mutant' vs 'wild type'
Cpr67Fb DOWN in 'CrebA null mutant' vs 'wild type'
ZnT35C UP in 'CrebA null mutant' vs 'wild type'
CG2444 UP in 'CrebA null mutant' vs 'wild type'
CG3513 UP in 'CrebA null mutant' vs 'wild type'
CG11893 UP in 'CrebA null mutant' vs 'wild type'
CG10126 UP in 'CrebA null mutant' vs 'wild type'
CG9698 DOWN in 'CrebA null mutant' vs 'wild type'
CG18067 UP in 'CrebA null mutant' vs 'wild type'
CG7912 UP in 'CrebA null mutant' vs 'wild type'
mre11 UP in 'CrebA null mutant' vs 'wild type'
MFS18 DOWN in 'CrebA null mutant' vs 'wild type'
CG18581 DOWN in 'CrebA null mutant' vs 'wild type'
CG15065 UP in 'CrebA null mutant' vs 'wild type'
park UP in 'CrebA null mutant' vs 'wild type'
CG12355 UP in 'CrebA null mutant' vs 'wild type'
CG2993 UP in 'CrebA null mutant' vs 'wild type'
CG10859 UP in 'CrebA null mutant' vs 'wild type'
CG9555 UP in 'CrebA null mutant' vs 'wild type'
CG2233 UP in 'CrebA null mutant' vs 'wild type'
Eip71CD UP in 'CrebA null mutant' vs 'wild type'
GstD5 DOWN in 'CrebA null mutant' vs 'wild type'
b6 UP in 'CrebA null mutant' vs 'wild type'
l(2)efl UP in 'CrebA null mutant' vs 'wild type'
CG11852 UP in 'CrebA null mutant' vs 'wild type'
Osi2 UP in 'CrebA null mutant' vs 'wild type'
CG2017 UP in 'CrebA null mutant' vs 'wild type'