GJA1
type: EnsemblDatabaseReference

GJA1
type: EnsemblDatabaseReference

(Sample) cell line/MCF-7aro
type: EFO_0002072
property type: cell line
property value: MCF-7aro

Analysis of 'androgen and letrozole' vs 'vehicle control'
type: MicroarrayDifferentialExpressionAnalysis

TEX14
type: EnsemblDatabaseReference

Analysis of 'androgen and anastrozole' vs 'vehicle control'
type: MicroarrayDifferentialExpressionAnalysis

ZBTB16
type: EnsemblDatabaseReference

208180_s_at
type: EFO_0005072
part of: A-AFFY-33

212651_at
type: EFO_0005072
part of: A-AFFY-33

205883_at
type: EFO_0005072
part of: A-AFFY-33

(Factor value) COMPOUND/androgen and tamoxifen
type: EFO_0000001
property type: COMPOUND
property value: androgen and tamoxifen

PPP3CA
type: EnsemblDatabaseReference

BCL6
type: EnsemblDatabaseReference

BCL6
type: EnsemblDatabaseReference

E-GEOD-2225
type: microarray 1colour mrna differential
RO_0002162: Homo sapiens
description: Anti-estrogens and aromatase inhibitors are important drugs in the treatment of estrogen-dependent breast cancer. In order to investigate the effects of these drugs on gene expression in breast cancer cells, we treated estrogen receptor-positive MCF-7 cells, stably transfected with the aromatase gene (known as MCF-7aro cells), with testosterone, 17ÃÂÃÂ²-estradiol, two aromatase inhibitors (letrozole and anastrozole), and an anti-estrogen (tamoxifen). Microarray analyses using Affymetrix Human Genome U133A GeneChips were carried out using total RNA isolated from the control and treated cells. When comparing the effect of each inhibitor on gene expression we observe that letrozole and anastrozole are more similar in terms of the genes they affect, compared to treatment with tamoxifen. The results of this study provide us with a better understanding of the actions of both aromatase inhibitors and anti-estrogens at the molecular level. We believe that the results of this study serve as the first step in identifying unique expression patterns following drug treatment, and that this will ultimately be useful in customizing patient treatment strategies for estrogen-dependent breast cancer. The data presented here have been processed using the R-Project Bioconductor statistical tools package using the affy library. The following were applied: RMA background correction, pmonly probe-level correction, quantile normalization, avgdiff summary method. Raw data is provided in the form of .CEL files.
uses data from: http://identifiers.org/pubmed/15831674

TEX14 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.53409463203898
p value: 0.00755066783480127
refers to: 221035_s_at
refers to: TEX14
t statistic: 10.5051329201562

PMP22 DOWN in 'estrogen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -2.18645272726032
p value: 0.0365782900959337
refers to: PMP22
refers to: 210139_s_at
t statistic: -7.25672098132321

RND3
type: EnsemblDatabaseReference

RND3
type: EnsemblDatabaseReference

213768_s_at
type: EFO_0005072
part of: A-AFFY-33

209985_s_at
type: EFO_0005072
part of: A-AFFY-33

(Assay) androgen treated - Rep 3
type: Assay
identifier: androgen treated - Rep 3
has participant: A-AFFY-33

FHL2 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.24607963206194
p value: 0.0418783829233828
refers to: FHL2
refers to: 202949_s_at
t statistic: 6.66963414430069

RHOBTB1 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.30084145290833
p value: 0.0417617207824052
refers to: RHOBTB1
refers to: 212651_at
t statistic: 6.75206058050405

RND3 DOWN in 'androgen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.16560500768907
p value: 0.0303535683900722
refers to: 212724_at
refers to: RND3
t statistic: -7.87163976316593

ASCL1 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.2931137635162
p value: 0.0425740568127809
refers to: ASCL1
refers to: 209985_s_at
t statistic: 6.57891640836534

(Factor value) COMPOUND/androgen and letrozole
type: EFO_0000001
property type: COMPOUND
property value: androgen and letrozole

NEBL
type: EnsemblDatabaseReference

RND3 DOWN in 'estrogen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.03329597043083
p value: 0.0245267854637379
refers to: RND3
refers to: 212724_at
t statistic: -8.41884948396341

NEBL
type: EnsemblDatabaseReference

209624_s_at
type: EFO_0005072
part of: A-AFFY-33

PPP3CA UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.13566284299164
p value: 0.00644632860124785
refers to: PPP3CA
refers to: 202457_s_at
t statistic: 8.43924452937635

208083_s_at
type: EFO_0005072
part of: A-AFFY-33

(Assay) estrogen treated - Rep 1
type: Assay
identifier: estrogen treated - Rep 1
has participant: A-AFFY-33

ASCL1 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 2.21343268698809
p value: 0.0203561563411898
refers to: ASCL1
refers to: 213768_s_at
t statistic: 8.50600856475418

HIST1H4H
type: EnsemblDatabaseReference

PMP22 DOWN in 'androgen and anastrozole' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -3.16982910699947
p value: 0.0398424419359387
refers to: PMP22
refers to: 210139_s_at
t statistic: -8.73738109636741

HIST1H4H
type: EnsemblDatabaseReference

MCCC2
type: EnsemblDatabaseReference

MCCC2
type: EnsemblDatabaseReference

209988_s_at
type: EFO_0005072
part of: A-AFFY-33

NEBL UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.45706309352719
p value: 0.0414900478614176
refers to: NEBL
refers to: 203961_at
t statistic: 5.37411069919324

209301_at
type: EFO_0005072
part of: A-AFFY-33

ZBTB16 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.06202108542644
p value: 0.00644632860124785
refers to: ZBTB16
refers to: 205883_at
t statistic: 8.34228774485583

FDFT1
type: EnsemblDatabaseReference

FDFT1
type: EnsemblDatabaseReference

211029_x_at
type: EFO_0005072
part of: A-AFFY-33

209987_s_at
type: EFO_0005072
part of: A-AFFY-33

(Assay) control - Rep 3
type: Assay
identifier: control - Rep 3
has participant: A-AFFY-33

(Factor value) COMPOUND/androgen
type: CHEBI_50113
property type: COMPOUND
property value: androgen

(Assay) androgen treated - Rep 1
type: Assay
identifier: androgen treated - Rep 1
has participant: A-AFFY-33

ID1
type: EnsemblDatabaseReference

ID1
type: EnsemblDatabaseReference

PPP3CA UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.12206034869281
p value: 0.0142643320975106
refers to: PPP3CA
refers to: 202429_s_at
t statistic: 6.93900772617001

221035_s_at
type: EFO_0005072
part of: A-AFFY-33

(Assay) A + tamoxifen - Rep 3
type: Assay
identifier: A + tamoxifen - Rep 3
has participant: A-AFFY-33

FGF18
type: EnsemblDatabaseReference

FGF18
type: EnsemblDatabaseReference

ITGB6 DOWN in 'androgen and tamoxifen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.69665312527325
p value: 0.00643312290779178
refers to: 208083_s_at
refers to: ITGB6
t statistic: -11.2237458850422

203961_at
type: EFO_0005072
part of: A-AFFY-33

RHOBTB1
type: EnsemblDatabaseReference

BCL6 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.45561435552109
p value: 0.00156231176591109
refers to: BCL6
refers to: 203140_at
t statistic: 12.5922075617641

(Assay) A + tamoxifen - Rep 2
type: Assay
identifier: A + tamoxifen - Rep 2
has participant: A-AFFY-33

GJA1 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 3.79270874138045
p value: 0.00212629395884052
refers to: 201667_at
refers to: GJA1
t statistic: 11.3588944723789

(Assay) A + letrozole - Rep 3
type: Assay
identifier: A + letrozole - Rep 3
has participant: A-AFFY-33

208647_at
type: EFO_0005072
part of: A-AFFY-33

Homo sapiens
type: OBI_0100026

(Assay) A + anastrozole - Rep 1
type: Assay
identifier: A + anastrozole - Rep 1
has participant: A-AFFY-33

TP53TG1 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.24855959859888
p value: 0.0498508443092196
refers to: TP53TG1
refers to: 209917_s_at
t statistic: 5.03126756521818

TP53TG1
type: EnsemblDatabaseReference

MATN2 DOWN in 'androgen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.12879005683848
p value: 0.0356553790457722
refers to: 202350_s_at
refers to: MATN2
t statistic: -7.23394861678168

TP53TG1
type: EnsemblDatabaseReference

210886_x_at
type: EFO_0005072
part of: A-AFFY-33

Analysis of 'androgen' vs 'vehicle control'
type: MicroarrayDifferentialExpressionAnalysis

Analysis of 'androgen and tamoxifen' vs 'vehicle control'
type: MicroarrayDifferentialExpressionAnalysis

TP53TG1 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.24550702251567
p value: 0.0498508443092196
refers to: TP53TG1
refers to: 210886_x_at
t statistic: 5.01390725776236

FHL2
type: EnsemblDatabaseReference

(Factor value) COMPOUND/androgen and anastrozole
type: EFO_0000001
property type: COMPOUND
property value: androgen and anastrozole

FHL2
type: EnsemblDatabaseReference

202429_s_at
type: EFO_0005072
part of: A-AFFY-33

(Assay) estrogen treated - Rep 3
type: Assay
identifier: estrogen treated - Rep 3
has participant: A-AFFY-33

NRIP1
type: EnsemblDatabaseReference

ASCL1 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 5.04092778690206
p value: 3.60711635127291E-5
refers to: 209988_s_at
refers to: ASCL1
t statistic: 27.6429801892611

PMP22
type: EnsemblDatabaseReference

MCCC2 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.61143002071592
p value: 0.0292965796284352
refers to: 209624_s_at
refers to: MCCC2
t statistic: 5.8224590268676

MATN2 DOWN in 'androgen and tamoxifen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.61461154248292
p value: 0.0203561563411898
refers to: MATN2
refers to: 202350_s_at
t statistic: -8.16239285021695

ITGB6 DOWN in 'androgen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.56370338238199
p value: 0.0303535683900722
refers to: ITGB6
refers to: 208083_s_at
t statistic: -9.76035249609785

ITGB6
type: EnsemblDatabaseReference

202949_s_at
type: EFO_0005072
part of: A-AFFY-33

ITGB6
type: EnsemblDatabaseReference

(Assay) androgen treated - Rep 2
type: Assay
identifier: androgen treated - Rep 2
has participant: A-AFFY-33

CA2 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 2.87262574000718
p value: 0.0063828606960345
refers to: CA2
refers to: 209301_at
t statistic: 8.95975904892597

SH3BGRL UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.26048930369766
p value: 0.017201628257213
refers to: SH3BGRL
refers to: 201311_s_at
t statistic: 6.58166255208045

(Assay) A + letrozole - Rep 2
type: Assay
identifier: A + letrozole - Rep 2
has participant: A-AFFY-33

FDFT1 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.38738428439653
p value: 0.0379666879364684
refers to: FDFT1
refers to: 208647_at
t statistic: 6.94288725704672

210139_s_at
type: EFO_0005072
part of: A-AFFY-33

209917_s_at
type: EFO_0005072
part of: A-AFFY-33

202457_s_at
type: EFO_0005072
part of: A-AFFY-33

FGF18 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.37363785659572
p value: 0.0090324492301946
refers to: 206987_x_at
refers to: FGF18
t statistic: 7.78535949268876

RHOBTB1
type: EnsemblDatabaseReference

(Assay) estrogen treated - Rep 2
type: Assay
identifier: estrogen treated - Rep 2
has participant: A-AFFY-33

PMP22 DOWN in 'androgen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -2.29708283981872
p value: 0.0319844555924385
refers to: PMP22
refers to: 210139_s_at
t statistic: -7.54727047357247

202600_s_at
type: EFO_0005072
part of: A-AFFY-33

SH3BGRL
type: EnsemblDatabaseReference

SH3BGRL
type: EnsemblDatabaseReference

(Assay) control - Rep 2
type: Assay
identifier: control - Rep 2
has participant: A-AFFY-33

ASCL1
type: EnsemblDatabaseReference

ASCL1
type: EnsemblDatabaseReference

CA2
type: EnsemblDatabaseReference

UGDH UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.28844302877284
p value: 0.0439256450058528
refers to: UGDH
refers to: 203343_at
t statistic: 5.27885881958603

CA2
type: EnsemblDatabaseReference

210950_s_at
type: EFO_0005072
part of: A-AFFY-33

PTGES
type: EnsemblDatabaseReference

PTGES
type: EnsemblDatabaseReference

UGDH
type: EnsemblDatabaseReference

202350_s_at
type: EFO_0005072
part of: A-AFFY-33

ITGB6 DOWN in 'estrogen' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.53707011648888
p value: 0.0179633478229279
refers to: 208083_s_at
refers to: ITGB6
t statistic: -9.81264866382153

(Assay) A + tamoxifen - Rep 1
type: Assay
identifier: A + tamoxifen - Rep 1
has participant: A-AFFY-33

FGF18 UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.34487391276013
p value: 0.00143376450891975
refers to: FGF18
refers to: 211029_x_at
t statistic: 14.0786593112381

Analysis of 'estrogen' vs 'vehicle control'
type: MicroarrayDifferentialExpressionAnalysis

PMP22 DOWN in 'androgen and letrozole' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -2.59398531017903
p value: 0.0261147522909191
refers to: 210139_s_at
refers to: PMP22
t statistic: -6.08280313133885

TEX14
type: EnsemblDatabaseReference

ASCL1 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 4.50690430011447
p value: 7.88388186108841E-4
refers to: ASCL1
refers to: 209987_s_at
t statistic: 16.1475433015894

NRIP1
type: EnsemblDatabaseReference

201311_s_at
type: EFO_0005072
part of: A-AFFY-33

PMP22
type: EnsemblDatabaseReference

208937_s_at
type: EFO_0005072
part of: A-AFFY-33

203343_at
type: EFO_0005072
part of: A-AFFY-33

A-AFFY-33
type: EFO_0000269

ZBTB16
type: EnsemblDatabaseReference

FDFT1 UP in 'androgen and tamoxifen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.52859503534734
p value: 0.0203561563411898
refers to: FDFT1
refers to: 210950_s_at
t statistic: 8.15022435135751

ID1 DOWN in 'androgen and letrozole' vs 'vehicle control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.18997136090019
p value: 0.00930942158084629
refers to: ID1
refers to: 208937_s_at
t statistic: -7.62546493651831

(Factor value) COMPOUND/vehicle control
type: EFO_0000001
property type: COMPOUND
property value: vehicle control

PPP3CA
type: EnsemblDatabaseReference

MATN2
type: EnsemblDatabaseReference

PTGES UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.53622901423976
p value: 0.0063828606960345
refers to: PTGES
refers to: 210367_s_at
t statistic: 8.72403826244656

MATN2
type: EnsemblDatabaseReference

206987_x_at
type: EFO_0005072
part of: A-AFFY-33

(Sample) cell type/breast cancer cell
type: EFO_0000001
property type: cell type
property value: breast cancer cell

NRIP1 UP in 'estrogen' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.19305444013008
p value: 0.0299820671814882
refers to: 202600_s_at
refers to: NRIP1
t statistic: 7.77765485726295

(Assay) A + anastrozole - Rep 2
type: Assay
identifier: A + anastrozole - Rep 2
has participant: A-AFFY-33

(Sample) organism/Homo sapiens
type: Homo sapiens
property type: organism
property value: Homo sapiens

(Assay) A + anastrozole - Rep 3
type: Assay
identifier: A + anastrozole - Rep 3
has participant: A-AFFY-33

201667_at
type: EFO_0005072
part of: A-AFFY-33

210367_s_at
type: EFO_0005072
part of: A-AFFY-33

HIST1H4H UP in 'androgen and letrozole' vs 'vehicle control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.07850036680068
p value: 0.0292965796284352
refers to: 208180_s_at
refers to: HIST1H4H
t statistic: 5.74362092771455

(Assay) control - Rep 1
type: Assay
identifier: control - Rep 1
has participant: A-AFFY-33

(Assay) A + letrozole - Rep 1
type: Assay
identifier: A + letrozole - Rep 1
has participant: A-AFFY-33

203140_at
type: EFO_0005072
part of: A-AFFY-33

212724_at
type: EFO_0005072
part of: A-AFFY-33

(Factor value) COMPOUND/estrogen
type: CHEBI_50114
property type: COMPOUND
property value: estrogen

UGDH
type: EnsemblDatabaseReference