Analysis of 'androgen and letrozole' vs 'vehicle control'
Analysis of 'androgen and anastrozole' vs 'vehicle control'
(Factor value) COMPOUND/androgen and tamoxifen
Anti-estrogens and aromatase inhibitors are important drugs in the treatment of estrogen-dependent breast cancer. In order to investigate the effects of these drugs on gene expression in breast cancer cells, we treated estrogen receptor-positive MCF-7 cells, stably transfected with the aromatase gene (known as MCF-7aro cells), with testosterone, 17ÃÂÃÂ²-estradiol, two aromatase inhibitors (letrozole and anastrozole), and an anti-estrogen (tamoxifen). Microarray analyses using Affymetrix Human Genome U133A GeneChips were carried out using total RNA isolated from the control and treated cells. When comparing the effect of each inhibitor on gene expression we observe that letrozole and anastrozole are more similar in terms of the genes they affect, compared to treatment with tamoxifen. The results of this study provide us with a better understanding of the actions of both aromatase inhibitors and anti-estrogens at the molecular level. We believe that the results of this study serve as the first step in identifying unique expression patterns following drug treatment, and that this will ultimately be useful in customizing patient treatment strategies for estrogen-dependent breast cancer. The data presented here have been processed using the R-Project Bioconductor statistical tools package using the affy library. The following were applied: RMA background correction, pmonly probe-level correction, quantile normalization, avgdiff summary method. Raw data is provided in the form of .CEL files.
TEX14 UP in 'androgen and tamoxifen' vs 'vehicle control'
PMP22 DOWN in 'estrogen' vs 'vehicle control'
FHL2 UP in 'androgen and tamoxifen' vs 'vehicle control'
RHOBTB1 UP in 'androgen and tamoxifen' vs 'vehicle control'
RND3 DOWN in 'androgen' vs 'vehicle control'
ASCL1 UP in 'androgen and tamoxifen' vs 'vehicle control'
(Factor value) COMPOUND/androgen and letrozole
RND3 DOWN in 'estrogen' vs 'vehicle control'
PPP3CA UP in 'androgen and letrozole' vs 'vehicle control'
PMP22 DOWN in 'androgen and anastrozole' vs 'vehicle control'
NEBL UP in 'androgen and letrozole' vs 'vehicle control'
ZBTB16 UP in 'androgen and letrozole' vs 'vehicle control'
ITGB6 DOWN in 'androgen and tamoxifen' vs 'vehicle control'
BCL6 UP in 'androgen and letrozole' vs 'vehicle control'
GJA1 UP in 'androgen and letrozole' vs 'vehicle control'
TP53TG1 UP in 'androgen and letrozole' vs 'vehicle control'
MATN2 DOWN in 'androgen' vs 'vehicle control'
Analysis of 'androgen' vs 'vehicle control'
Analysis of 'androgen and tamoxifen' vs 'vehicle control'
(Factor value) COMPOUND/androgen and anastrozole
MCCC2 UP in 'androgen and letrozole' vs 'vehicle control'
MATN2 DOWN in 'androgen and tamoxifen' vs 'vehicle control'
ITGB6 DOWN in 'androgen' vs 'vehicle control'
CA2 UP in 'androgen and letrozole' vs 'vehicle control'
SH3BGRL UP in 'androgen and letrozole' vs 'vehicle control'
FDFT1 UP in 'androgen and tamoxifen' vs 'vehicle control'
FGF18 UP in 'androgen and letrozole' vs 'vehicle control'
PMP22 DOWN in 'androgen' vs 'vehicle control'
UGDH UP in 'androgen and letrozole' vs 'vehicle control'
ITGB6 DOWN in 'estrogen' vs 'vehicle control'
Analysis of 'estrogen' vs 'vehicle control'
PMP22 DOWN in 'androgen and letrozole' vs 'vehicle control'
ID1 DOWN in 'androgen and letrozole' vs 'vehicle control'
PTGES UP in 'androgen and letrozole' vs 'vehicle control'
NRIP1 UP in 'estrogen' vs 'vehicle control'
HIST1H4H UP in 'androgen and letrozole' vs 'vehicle control'