PLEKHA3 DOWN in 'pre-miR-17' vs 'control pre-miR'
QKI UP in 'pre-miR-17' vs 'control pre-miR'
Analysis of 'pre-miR-17' vs 'control pre-miR'
DOK5 UP in 'pre-miR-17' vs 'control pre-miR'
TGFBR2 DOWN in 'pre-miR-17' vs 'control pre-miR'
CFL2 DOWN in 'pre-miR-17' vs 'control pre-miR'
PCDH17 UP in 'pre-miR-17' vs 'control pre-miR'
JAK1 DOWN in 'pre-miR-17' vs 'control pre-miR'
AKTIP DOWN in 'pre-miR-17' vs 'control pre-miR'
M6PR DOWN in 'pre-miR-17' vs 'control pre-miR'
CMTR2 DOWN in 'pre-miR-17' vs 'control pre-miR'
ALDH1A1 UP in 'pre-miR-17' vs 'control pre-miR'
EIF5A2 DOWN in 'pre-miR-17' vs 'control pre-miR'
MOB1A DOWN in 'pre-miR-17' vs 'control pre-miR'
MKRN1 DOWN in 'pre-miR-17' vs 'control pre-miR'
TSPAN8 UP in 'pre-miR-17' vs 'control pre-miR'
PAPPA UP in 'pre-miR-17' vs 'control pre-miR'
OXR1 DOWN in 'pre-miR-17' vs 'control pre-miR'
STC1 DOWN in 'pre-miR-17' vs 'control pre-miR'
(Factor value) RNA_INTERFERENCE/control pre-miR
SMIM14 DOWN in 'pre-miR-17' vs 'control pre-miR'
GPR137B DOWN in 'pre-miR-17' vs 'control pre-miR'
ERLIN2 UP in 'pre-miR-17' vs 'control pre-miR'
TMEM87B UP in 'pre-miR-17' vs 'control pre-miR'
KIT DOWN in 'pre-miR-17' vs 'control pre-miR'
TMEM19 DOWN in 'pre-miR-17' vs 'control pre-miR'
ADAM9 DOWN in 'pre-miR-17' vs 'control pre-miR'
(Sample) cell type/umbilical vein endothelial cell
SLC16A9 DOWN in 'pre-miR-17' vs 'control pre-miR'
EFCAB14 DOWN in 'pre-miR-17' vs 'control pre-miR'
ELK4 DOWN in 'pre-miR-17' vs 'control pre-miR'
HCCS DOWN in 'pre-miR-17' vs 'control pre-miR'
HDHD1 DOWN in 'pre-miR-17' vs 'control pre-miR'
GBP3 DOWN in 'pre-miR-17' vs 'control pre-miR'
LPAR1 UP in 'pre-miR-17' vs 'control pre-miR'
SGPP1 UP in 'pre-miR-17' vs 'control pre-miR'
CLOCK DOWN in 'pre-miR-17' vs 'control pre-miR'
PPM1A DOWN in 'pre-miR-17' vs 'control pre-miR'
HMGCS1 UP in 'pre-miR-17' vs 'control pre-miR'
NAGK DOWN in 'pre-miR-17' vs 'control pre-miR'
MALAT1 DOWN in 'pre-miR-17' vs 'control pre-miR'
GIPC2 DOWN in 'pre-miR-17' vs 'control pre-miR'
ZBTB41 DOWN in 'pre-miR-17' vs 'control pre-miR'
RASSF2 DOWN in 'pre-miR-17' vs 'control pre-miR'
MAP3K2 DOWN in 'pre-miR-17' vs 'control pre-miR'
CDC37L1 DOWN in 'pre-miR-17' vs 'control pre-miR'
RNF41 UP in 'pre-miR-17' vs 'control pre-miR'
AGO1 DOWN in 'pre-miR-17' vs 'control pre-miR'
DAZAP2 DOWN in 'pre-miR-17' vs 'control pre-miR'
FYCO1 DOWN in 'pre-miR-17' vs 'control pre-miR'
CDK14 UP in 'pre-miR-17' vs 'control pre-miR'
USP46 DOWN in 'pre-miR-17' vs 'control pre-miR'
ANKRD46 UP in 'pre-miR-17' vs 'control pre-miR'
ASPH UP in 'pre-miR-17' vs 'control pre-miR'
MEST UP in 'pre-miR-17' vs 'control pre-miR'
TMEM64 DOWN in 'pre-miR-17' vs 'control pre-miR'
LCOR DOWN in 'pre-miR-17' vs 'control pre-miR'
MGARP UP in 'pre-miR-17' vs 'control pre-miR'
BMP4 UP in 'pre-miR-17' vs 'control pre-miR'
ITPRIPL2 DOWN in 'pre-miR-17' vs 'control pre-miR'
GNPDA2 DOWN in 'pre-miR-17' vs 'control pre-miR'
KATNAL1 DOWN in 'pre-miR-17' vs 'control pre-miR'
(Factor value) RNA_INTERFERENCE/pre-miR-17
PDCD1LG2 DOWN in 'pre-miR-17' vs 'control pre-miR'
LINC00116 DOWN in 'pre-miR-17' vs 'control pre-miR'
RNH1 DOWN in 'pre-miR-17' vs 'control pre-miR'
TRIB2 UP in 'pre-miR-17' vs 'control pre-miR'
FZD5 UP in 'pre-miR-17' vs 'control pre-miR'
C7orf60 DOWN in 'pre-miR-17' vs 'control pre-miR'
CYBRD1 DOWN in 'pre-miR-17' vs 'control pre-miR'
TBCEL DOWN in 'pre-miR-17' vs 'control pre-miR'
TGFB2 UP in 'pre-miR-17' vs 'control pre-miR'
SGPL1 DOWN in 'pre-miR-17' vs 'control pre-miR'
TBC1D9 DOWN in 'pre-miR-17' vs 'control pre-miR'
NKIRAS1 DOWN in 'pre-miR-17' vs 'control pre-miR'
MARCH3 DOWN in 'pre-miR-17' vs 'control pre-miR'
MicroRNAs are endogenously expressed small non-coding RNAs that regulate gene expression on the posttranscriptional level. The miR-17-92 cluster (encoding miR-17, -18a, -19a/b, -20a and miR-92a) is highly expressed in tumor cells and is up-regulated by ischemia. Whereas miR-92a was recently identified as negative regulator of angiogenesis, the specific functions of the other members of the cluster are less clear. Here we demonstrate that overexpression of miR-17, -18a, -19a and -20a significantly inhibited 3D spheroid sprouting in vitro, whereas inhibition of miR-17, -18a and -20a augmented endothelial cell (EC) sprout formation. Inhibition of miR-17 and miR-20a in vivo using antagomirs significantly increased the number of perfused vessels in matrigel plugs, whereas antagomirs, that specifically target miR-18a and miR-19a were less effective. However, systemic inhibition of miR-17/20 did not affect tumor angiogenesis. Further mechanistic studies showed that miR-17/20 targets several pro-angiogenic genes. Specifically, Janus kinase 1 (Jak1) was shown to be a direct target of miR-17. In summary, we show that miR-17/20 exhibit a cell intrinsic anti-angiogenic activity in ECs. Inhibition of miR-17/20 specifically augmented neovascularization of matrigel plugs, but did not affect tumor angiogenesis indicating a context-dependent regulation of angiogenesis by miR-17/20 in vivo. 6 samples of 3 independent experiments (n=3): per experiment Pre-miR-Co (10 nM, Ambion) and Pre-miR-17 (10 nM, Ambion) transfected HUVEC 24h after transfection
RNF149 DOWN in 'pre-miR-17' vs 'control pre-miR'