DSE4 DOWN in 'PUN1 mutant' vs 'wild type'
ARG1 DOWN in 'PUN1 mutant' vs 'wild type'
TMA10 DOWN in 'PUN1 mutant' vs 'wild type'
SCW11 DOWN in 'PUN1 mutant' vs 'wild type'
DSE2 DOWN in 'PUN1 mutant' vs 'wild type'
PRY3 DOWN in 'PUN1 mutant' vs 'wild type'
PAU24 UP in 'PUN1 mutant' vs 'wild type'
YJR149W UP in 'PUN1 mutant' vs 'wild type'
YKL071W UP in 'PUN1 mutant' vs 'wild type'
YAL037W UP in 'PUN1 mutant' vs 'wild type'
YDL160C-A UP in 'PUN1 mutant' vs 'wild type'
RTS3 DOWN in 'PUN1 mutant' vs 'wild type'
YKR075C DOWN in 'PUN1 mutant' vs 'wild type'
GAT1 DOWN in 'PUN1 mutant' vs 'wild type'
COS10 DOWN in 'PUN1 mutant' vs 'wild type'
IRC18 UP in 'PUN1 mutant' vs 'wild type'
MPC54 UP in 'PUN1 mutant' vs 'wild type'
OAC1 DOWN in 'PUN1 mutant' vs 'wild type'
(Sample) organism/Saccharomyces cerevisiae
SIP18 UP in 'PUN1 mutant' vs 'wild type'
YLR040C UP in 'PUN1 mutant' vs 'wild type'
MET13 DOWN in 'PUN1 mutant' vs 'wild type'
FUR4 DOWN in 'PUN1 mutant' vs 'wild type'
Analysis of 'PUN1 mutant' vs 'wild type'
RDH54 UP in 'PUN1 mutant' vs 'wild type'
LEU1 DOWN in 'PUN1 mutant' vs 'wild type'
DSE1 DOWN in 'PUN1 mutant' vs 'wild type'
EGT2 DOWN in 'PUN1 mutant' vs 'wild type'
ALD5 DOWN in 'PUN1 mutant' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
NRG2 DOWN in 'PUN1 mutant' vs 'wild type'
STE14 UP in 'PUN1 mutant' vs 'wild type'
RTT107 UP in 'PUN1 mutant' vs 'wild type'
YIL055C UP in 'PUN1 mutant' vs 'wild type'
YDR222W UP in 'PUN1 mutant' vs 'wild type'
BSC5 DOWN in 'PUN1 mutant' vs 'wild type'
PUN1 DOWN in 'PUN1 mutant' vs 'wild type'
YLL053C DOWN in 'PUN1 mutant' vs 'wild type'
YHR033W UP in 'PUN1 mutant' vs 'wild type'
YLR031W UP in 'PUN1 mutant' vs 'wild type'
Yeast filamentous growth is a stress response to conditions of nitrogen deprivation, wherein yeast colonies form pseudohyphal filaments of elongated and connected cells. As proteins mediating adhesion and transport are required for this growth transition, the protein complement at the yeast cell periphery plays a critical and tightly regulated role in enabling pseudohyphal filamentation. To identify proteins differentially abundant at the yeast cell periphery during pseudohyphal growth, we generated quantitative proteomic profiles of plasma membrane protein preparations under conditions of vegetative growth and filamentation. By iTRAQ chemistry and two-dimensional LC-MS/MS, we profiled 2,463 peptides and 356 proteins, from which we identified eleven differentially abundant proteins that localize to the yeast cell periphery. This protein set includes Ylr414cp, herein renamed Pun1p, a previously uncharacterized protein localized to the plasma membrane compartment of Can1 (MCC). Pun1p abundance is increased two-fold under conditions of nitrogen stress, and deletion of PUN1 abolishes filamentous growth in haploids and diploids; pun1D mutants are non-invasive, lack surface-spread filamentation, grow slowly, and exhibit impaired cell adhesion. Conversely, overexpression of PUN1 results in exaggerated cell elongation under conditions of nitrogen stress. PUN1 contributes to yeast nitrogen signaling, as pun1D mutants misregulate amino acid biosynthetic genes during nitrogen deprivation. By chromatin immunoprecipitation and RT-PCR, we find that the filamentous growth factor Mss11p directly binds to the PUN1 promoter and regulates its transcription. In total, this study provides the first profile of protein abundance during pseudohyphal growth, identifying a previously uncharacterized MCC protein required for wild-type nitrogen signaling and filamentous growth. For this study, we constructed a homozygous diploid strain in the filamentous ÃÂ£1278b background deleted for PUN1; a wild-type diploid strain of the same background served as the control. Both strains were grown under conditions of low nitrogen, and RNA was extracted from three biological replicates of each strain after identical culturing. The RNA samples were analyzed using affymerix DNA microarrays.
AQY2 DOWN in 'PUN1 mutant' vs 'wild type'
CTS1 DOWN in 'PUN1 mutant' vs 'wild type'
DSE3 DOWN in 'PUN1 mutant' vs 'wild type'
YNR034W-A DOWN in 'PUN1 mutant' vs 'wild type'
INO2 DOWN in 'PUN1 mutant' vs 'wild type'
DIC1 DOWN in 'PUN1 mutant' vs 'wild type'
ISU2 DOWN in 'PUN1 mutant' vs 'wild type'
MF(ALPHA)2 UP in 'PUN1 mutant' vs 'wild type'
RGI1 DOWN in 'PUN1 mutant' vs 'wild type'
MF(ALPHA)1 UP in 'PUN1 mutant' vs 'wild type'
ICY2 DOWN in 'PUN1 mutant' vs 'wild type'
ECM34 UP in 'PUN1 mutant' vs 'wild type'
YCR061W DOWN in 'PUN1 mutant' vs 'wild type'
STR3 DOWN in 'PUN1 mutant' vs 'wild type'
LEU4 DOWN in 'PUN1 mutant' vs 'wild type'
ARG3 DOWN in 'PUN1 mutant' vs 'wild type'
YLR307C-A UP in 'PUN1 mutant' vs 'wild type'
RPI1 DOWN in 'PUN1 mutant' vs 'wild type'
YBL029W DOWN in 'PUN1 mutant' vs 'wild type'
MET6 DOWN in 'PUN1 mutant' vs 'wild type'
HMRA1 DOWN in 'PUN1 mutant' vs 'wild type'
MAE1 DOWN in 'PUN1 mutant' vs 'wild type'
YNL194C UP in 'PUN1 mutant' vs 'wild type'
CPA2 DOWN in 'PUN1 mutant' vs 'wild type'
LEU2 DOWN in 'PUN1 mutant' vs 'wild type'
STE18 UP in 'PUN1 mutant' vs 'wild type'
YLR042C UP in 'PUN1 mutant' vs 'wild type'
IME4 DOWN in 'PUN1 mutant' vs 'wild type'
FLO10 UP in 'PUN1 mutant' vs 'wild type'
YNR014W DOWN in 'PUN1 mutant' vs 'wild type'
BAT1 DOWN in 'PUN1 mutant' vs 'wild type'