KING1 UP in 'sex1-3 mutation' vs 'wild type'
AT1G05340 UP in 'sex1-3 mutation' vs 'wild type'
AGP9 DOWN in 'sex1-3 mutation' vs 'wild type'
AT5G12420 UP in 'sex1-3 mutation' vs 'wild type'
BAT5 DOWN in 'sex1-3 mutation' vs 'wild type'
ELP DOWN in 'sex1-3 mutation' vs 'wild type'
TAT3 DOWN in 'sex1-3 mutation' vs 'wild type'
AT1G25400 UP in 'sex1-3 mutation' vs 'wild type'
AT1G66760 UP in 'sex1-3 mutation' vs 'wild type'
AT2G33830 UP in 'sex1-3 mutation' vs 'wild type'
ERD5 UP in 'sex1-3 mutation' vs 'wild type'
Analysis of 'ptpkis2 mutation' vs 'wild type'
AT4G35320 DOWN in 'sex1-3 mutation' vs 'wild type'
AT2G30600 UP in 'sex1-3 mutation' vs 'wild type'
STP1 UP in 'sex1-3 mutation' vs 'wild type'
BGAL3 DOWN in 'sex1-3 mutation' vs 'wild type'
GRP3S UP in 'sex1-3 mutation' vs 'wild type'
ALDH7B4 UP in 'sex1-3 mutation' vs 'wild type'
B5 #1 DOWN in 'sex1-3 mutation' vs 'wild type'
AT1G80160 UP in 'sex1-3 mutation' vs 'wild type'
AT1G13650 DOWN in 'sex1-3 mutation' vs 'wild type'
AGT3 UP in 'sex1-3 mutation' vs 'wild type'
AT5G20700 DOWN in 'sex1-3 mutation' vs 'wild type'
In starchless mutants of Arabidopsis, pgm, and wild type, Col0, there are small differences in transcript levels at the end of the day, but large differences at the end of the night. Many of the transcripts of genes involved in biosynthesis and growth are repressed in the pgm mutant (Thimm et al., 2004). It is hypothesized that a transient depletion in sugars at the end of the night inhibits carbon utilization at the beginning of the day (Gibon et al., 2004). In order to test if the same inhibition of transcripts involved in biosynthesis and growth also occurs in starch excess mutants of Arabidopsis, Affymetrix arrays will be generated for WT (Col0), and the following mutants: sex4-3 (Salk 102567), ptpkis2 (Salk 053285), and sex1-3 (Yu et al., 2001). WT, sex4-3, and ptpkis2 plants were grown for 30 days and sex1-3 plants were grown for 36 days. All plants were grown in a growth chamber with a 12 hour photoperiod, quantum flux 100 mol m-2s-1, relative humidity was at least 75%, and a constant temperature of 20oC. Three leaves from 8 different plants of each genotype were harvested using a green safe lamp 0.5 hours before the lights came on in the morning and immediately frozen in LN2. Leaf material was powered using a mortar and pestle and RNA was extracted using a Purescript RNA isolation kit (Gentra Systems, MN, USA). Samples were then treated with DNaseI (Invitrogen, CA, USA). 12 samples were used in this experiment.
AT1G75900 DOWN in 'sex1-3 mutation' vs 'wild type'
AT1G62480 UP in 'sex1-3 mutation' vs 'wild type'
AtSIP2 UP in 'sex1-3 mutation' vs 'wild type'
ATSDI1 UP in 'ptpkis2 mutation' vs 'wild type'
CRRSP38 UP in 'sex1-3 mutation' vs 'wild type'
AT2G20670 DOWN in 'ptpkis2 mutation' vs 'wild type'
ATMYBL2 UP in 'sex1-3 mutation' vs 'wild type'
AT4G35320 UP in 'ptpkis2 mutation' vs 'wild type'
UGT89C1 UP in 'ptpkis2 mutation' vs 'wild type'
AT5G44530 DOWN in 'sex1-3 mutation' vs 'wild type'
CCOAMT UP in 'sex1-3 mutation' vs 'wild type'
AT2G05540 UP in 'sex1-3 mutation' vs 'wild type'
FLA8 DOWN in 'sex1-3 mutation' vs 'wild type'
CYP83A1 DOWN in 'sex1-3 mutation' vs 'wild type'
AKINBETA1 UP in 'sex1-3 mutation' vs 'wild type'
QQS DOWN in 'sex1-3 mutation' vs 'wild type'
GRXS11 DOWN in 'sex1-3 mutation' vs 'wild type'
AT1G10140 UP in 'sex1-3 mutation' vs 'wild type'
GA4 DOWN in 'sex1-3 mutation' vs 'wild type'
IPMI1 DOWN in 'sex1-3 mutation' vs 'wild type'
TBL26 DOWN in 'sex1-3 mutation' vs 'wild type'
ATSDI1 UP in 'sex4-3 mutation' vs 'wild type'
CCL UP in 'sex1-3 mutation' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
ATMYBL2 DOWN in 'ptpkis2 mutation' vs 'wild type'
DYL1 UP in 'sex1-3 mutation' vs 'wild type'
AKN2 DOWN in 'sex1-3 mutation' vs 'wild type'
SWEET17 DOWN in 'sex1-3 mutation' vs 'wild type'
Analysis of 'sex1-3 mutation' vs 'wild type'
GST30 DOWN in 'sex1-3 mutation' vs 'wild type'
ATCSLA09 DOWN in 'sex1-3 mutation' vs 'wild type'
ATBCAT-2 UP in 'sex1-3 mutation' vs 'wild type'
AT3G21190 DOWN in 'sex1-3 mutation' vs 'wild type'
ATGSTU20 DOWN in 'sex1-3 mutation' vs 'wild type'
AKR4C8 UP in 'sex1-3 mutation' vs 'wild type'
AT1G04040 DOWN in 'sex1-3 mutation' vs 'wild type'
ATBCB UP in 'sex1-3 mutation' vs 'wild type'
UGE1 UP in 'sex1-3 mutation' vs 'wild type'
AT1G76590 UP in 'sex1-3 mutation' vs 'wild type'
AT2G21220 DOWN in 'sex1-3 mutation' vs 'wild type'
SEX1 DOWN in 'sex1-3 mutation' vs 'wild type'
AT3G54400 DOWN in 'sex1-3 mutation' vs 'wild type'
MCCA UP in 'sex1-3 mutation' vs 'wild type'
PCNA2 UP in 'sex1-3 mutation' vs 'wild type'
AKINBETA1 DOWN in 'ptpkis2 mutation' vs 'wild type'
DIN4 UP in 'sex1-3 mutation' vs 'wild type'
AT1G79700 UP in 'sex1-3 mutation' vs 'wild type'
IVD UP in 'sex1-3 mutation' vs 'wild type'
AT2G20670 UP in 'sex1-3 mutation' vs 'wild type'
ENODL19 DOWN in 'sex1-3 mutation' vs 'wild type'
CYP96A4 UP in 'ptpkis2 mutation' vs 'wild type'
AT1G09500 UP in 'sex1-3 mutation' vs 'wild type'
SVL1 DOWN in 'sex1-3 mutation' vs 'wild type'
AT3G15450 UP in 'sex1-3 mutation' vs 'wild type'
AT4G37180 UP in 'sex1-3 mutation' vs 'wild type'
GLX2-3 UP in 'sex1-3 mutation' vs 'wild type'
TBL26 UP in 'ptpkis2 mutation' vs 'wild type'
AT1G72060 UP in 'sex1-3 mutation' vs 'wild type'
AT3G15630 UP in 'sex1-3 mutation' vs 'wild type'
(Sample) developmental stage/Boyes key 3.7
ATRL5 UP in 'sex1-3 mutation' vs 'wild type'
AT3G54400 UP in 'ptpkis2 mutation' vs 'wild type'
ATMGL UP in 'sex1-3 mutation' vs 'wild type'
XTH8 DOWN in 'sex1-3 mutation' vs 'wild type'
PLP2 UP in 'sex1-3 mutation' vs 'wild type'
Analysis of 'sex4-3 mutation' vs 'wild type'
AT4G33960 DOWN in 'sex1-3 mutation' vs 'wild type'
AT1G07040 UP in 'sex1-3 mutation' vs 'wild type'
(Factor value) GENOTYPE/ptpkis2 mutation
AT4G30020 DOWN in 'sex1-3 mutation' vs 'wild type'
STP1 DOWN in 'ptpkis2 mutation' vs 'wild type'
BGL1 DOWN in 'sex1-3 mutation' vs 'wild type'
ATPSK4 UP in 'sex1-3 mutation' vs 'wild type'
BGAL8 DOWN in 'sex1-3 mutation' vs 'wild type'
AT1G30820 UP in 'sex1-3 mutation' vs 'wild type'
AT3G15450 DOWN in 'ptpkis2 mutation' vs 'wild type'
ASN1 UP in 'sex1-3 mutation' vs 'wild type'
AT3G14060 UP in 'sex1-3 mutation' vs 'wild type'