(Factor value) GENOTYPE/wild type genotype
AGO2 DOWN in 'AtATM3 mutant' vs 'wild type'
Analysis of 'AtATM3 mutant' vs 'wild type'
AT3G45730 DOWN in 'AtATM3 mutant' vs 'wild type'
AT1G59660 DOWN in 'AtATM3 mutant' vs 'wild type'
APP DOWN in 'AtATM3 mutant' vs 'wild type'
ARAC7 DOWN in 'AtATM3 mutant' vs 'wild type'
GMI1 DOWN in 'AtATM3 mutant' vs 'wild type'
RCE2 DOWN in 'AtATM3 mutant' vs 'wild type'
PARP2 DOWN in 'AtATM3 mutant' vs 'wild type'
AT1G80850 DOWN in 'AtATM3 mutant' vs 'wild type'
ATBRCA1 DOWN in 'AtATM3 mutant' vs 'wild type'
NRAMP3 DOWN in 'AtATM3 mutant' vs 'wild type'
AT1G23710 DOWN in 'AtATM3 mutant' vs 'wild type'
TRFL3 DOWN in 'AtATM3 mutant' vs 'wild type'
(Sample) developmental stage/Boyes key 1.04
AT3G14560 DOWN in 'AtATM3 mutant' vs 'wild type'
AT3G07800 DOWN in 'AtATM3 mutant' vs 'wild type'
anac053 DOWN in 'AtATM3 mutant' vs 'wild type'
ATCP1 DOWN in 'AtATM3 mutant' vs 'wild type'
WRR4 DOWN in 'AtATM3 mutant' vs 'wild type'
KCS17 DOWN in 'AtATM3 mutant' vs 'wild type'
TRFL10 DOWN in 'AtATM3 mutant' vs 'wild type'
RS31a DOWN in 'AtATM3 mutant' vs 'wild type'
AT2G47680 DOWN in 'AtATM3 mutant' vs 'wild type'
AT4G25580 DOWN in 'AtATM3 mutant' vs 'wild type'
WRKY25 DOWN in 'AtATM3 mutant' vs 'wild type'
AT4G19130 DOWN in 'AtATM3 mutant' vs 'wild type'
XRI DOWN in 'AtATM3 mutant' vs 'wild type'
CIPK11 DOWN in 'AtATM3 mutant' vs 'wild type'
ATM DOWN in 'AtATM3 mutant' vs 'wild type'
AT1G07500 DOWN in 'AtATM3 mutant' vs 'wild type'
RecQl3 DOWN in 'AtATM3 mutant' vs 'wild type'
AT2G45460 DOWN in 'AtATM3 mutant' vs 'wild type'
TFIIB DOWN in 'AtATM3 mutant' vs 'wild type'
AT3G42860 DOWN in 'AtATM3 mutant' vs 'wild type'
AT1G17360 DOWN in 'AtATM3 mutant' vs 'wild type'
AT5G27760 DOWN in 'AtATM3 mutant' vs 'wild type'
ATM plays an important role in the response of plants to forms of DNA damage that cause DNA double-strand breaks. Arabidopsis ATM has been shown to be important for the transcriptional induction of several DNA repair genes including AtRAD51 (Garcia et al 2003 Plant Cell 15, 119-132). In our experiment we compare the transcript profiles of wild type and mutant plants exposed to 10Gy X-rays (dose rate ~0.8 Gy/min). Plants will be grown on 0.5*MS + 1%sucrose at 22degC under short (9h) day conditions and treated after 5h light. We will irradiate wild type (Col-0) and atatm-3 mutant (N589805) seedlings at growth stage 1.06 and harvest tissue 1h post irradiation. RNA will be isolated using the SV RNA isolation kit (Promega). Comparison of the transcript profiles will then allow AtATM-dependent X-ray inducible transcripts to be identified. 6 samples (3 wild type, 3 mutant) were used in this experiment.
AHP2 DOWN in 'AtATM3 mutant' vs 'wild type'
TSO2 DOWN in 'AtATM3 mutant' vs 'wild type'
AT5G67460 DOWN in 'AtATM3 mutant' vs 'wild type'
POLD4 DOWN in 'AtATM3 mutant' vs 'wild type'
AT3G10930 DOWN in 'AtATM3 mutant' vs 'wild type'
CYC1 DOWN in 'AtATM3 mutant' vs 'wild type'
AT1G72790 DOWN in 'AtATM3 mutant' vs 'wild type'