265817_at
type: EFO_0005072
part of: A-AFFY-2

246231_at
type: EFO_0005072
part of: A-AFFY-2

AT4G12490 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.9805993
p value: 0.00124149936692459
refers to: AT4G12490
refers to: 254832_at
t statistic: 9.86024747886865

AT4G37080
type: EnsemblDatabaseReference

RAP2.3 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.18311666666667
p value: 5.1274288054914E-5
refers to: RAP2.3
refers to: 258434_at
t statistic: 17.596876877372

NAP UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.09098193333333
p value: 0.00895565804983488
refers to: 256300_at
refers to: NAP
t statistic: 6.32906943619845

AT4G16260
type: EnsemblDatabaseReference

ATMYB90 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.0186305
p value: 0.0037285549447627
refers to: 260140_at
refers to: ATMYB90
t statistic: 8.0079397340288

ATTI1
type: EnsemblDatabaseReference

AT4G12490
type: EnsemblDatabaseReference

A-AFFY-2
type: EFO_0000269

RAP2.3
type: EnsemblDatabaseReference

CAX3
type: EnsemblDatabaseReference

ATGSTF12
type: EnsemblDatabaseReference

SAG21 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.01452466666667
p value: 0.00124962542125898
refers to: SAG21
refers to: 255479_at
t statistic: 9.742561900064

NAP
type: EnsemblDatabaseReference

(Assay) GSM476822
type: Assay
has participant: A-AFFY-2

(Assay) GSM476821
type: Assay
has participant: A-AFFY-2

FPP4
type: EnsemblDatabaseReference

AT2G05540
type: EnsemblDatabaseReference

255479_at
type: EFO_0005072
part of: A-AFFY-2

AT2G05540 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.61247996666667
p value: 8.48041343780541E-4
refers to: 265511_at
refers to: AT2G05540
t statistic: 10.6975198847654

AT4G37080
type: EnsemblDatabaseReference

E-GEOD-19244
type: microarray 1colour mrna differential
RO_0002162: Arabidopsis thaliana
description: Cysteine occupies a central position in plant metabolism due to its biochemical functions. Arabidopsis thaliana cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes that catalyze the biosynthesis of cysteine. Because they are localized in the cytosol, plastids and mitochondria, this results in multiple subcellular cysteine pools. Much progress has been made on the most abundant OASTL enzymes; however, information on the less abundant OASTL-like proteins has been scarce. To unequivocally establish the enzymatic reaction catalyzed by the minor cytosolic OASTL isoform CS-LIKE (AT5G28030), we expressed this enzyme in bacteria and characterized the purified recombinant protein. Our results demonstrate that CS-LIKE catalyzes the desulfuration of L-cysteine to sulfide plus ammonia and pyruvate. Thus, CS-LIKE is a novel L-cysteine desulfhydrase (EC 4.4.1.1), and we propose to designate it DES1. The impact and functionality of DES1 in cysteine metabolism was revealed by the phenotype of the T-DNA insertion mutants des1-1 and des1-2. Mutation of the DES1 gene leads to premature leaf senescence, as demonstrated by the increased expression of senescence-associated genes and transcription factors. Also, the absence of DES1 significantly reduces the total cysteine desulfuration activity in leaves, and there is a concomitant increase in the total cysteine content. As a consequence, the expression levels of sulfur-responsive genes are de-regulated, and the mutant plants show enhanced antioxidant defenses and tolerance to conditions that promote oxidative stress. Our results suggest that DES1 from Arabidopsis is an L-cysteine desulfhydrase involved in maintaining cysteine homeostasis, mainly at late developmental stages or under environmental perturbations. Using the Affymetrix ATH1 GeneChips, we performed a comparative transcriptomic analysis on leaves of the des1-1 and wild type plants grown on soil for three weeks. Three biological replicates were performed for each…
uses data from: http://identifiers.org/pubmed/21988475

ATTI1 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.060056
p value: 0.0037285549447627
refers to: ATTI1
refers to: 260551_at
t statistic: 8.01520494239583

AT4G16260
type: EnsemblDatabaseReference

AT1G49500
type: EnsemblDatabaseReference

256300_at
type: EFO_0005072
part of: A-AFFY-2

AT4G12490
type: EnsemblDatabaseReference

CAX3 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 2.38943276666667
p value: 5.1274288054914E-5
refers to: CAX3
refers to: 246302_at
t statistic: 17.2423261477052

265511_at
type: EFO_0005072
part of: A-AFFY-2

RAP2.3
type: EnsemblDatabaseReference

(Sample) genotype/des1-1 mutant
type: EFO_0000001
property type: genotype
property value: des1-1 mutant

ATGSTF12
type: EnsemblDatabaseReference

PDF1.2b
type: EnsemblDatabaseReference

(Assay) GSM476826
type: Assay
has participant: A-AFFY-2

AT1G22060
type: EnsemblDatabaseReference

260140_at
type: EFO_0005072
part of: A-AFFY-2

PDF1.2b UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 2.430353
p value: 5.1274288054914E-5
refers to: PDF1.2b
refers to: 257365_x_at
t statistic: 17.7027024256481

262399_at
type: EFO_0005072
part of: A-AFFY-2

AT1G65010
type: EnsemblDatabaseReference

NAP
type: EnsemblDatabaseReference

(Sample) organism part/leaf
type: EFO_0000993
property type: organism part
property value: leaf

(Factor value) GENOTYPE/des1-1 mutant
type: EFO_0000001
property type: GENOTYPE
property value: des1-1 mutant

258791_at
type: EFO_0005072
part of: A-AFFY-2

AT2G05540
type: EnsemblDatabaseReference

SEN1 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.0116314
p value: 0.00459738199129236
refers to: SEN1
refers to: 253161_at
t statistic: 7.55198468343093

GRP3S UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.49080753333333
p value: 2.70151757326382E-4
refers to: GRP3S
refers to: 263046_at
t statistic: 13.1980527523958

SEN1
type: EnsemblDatabaseReference

GRP3S
type: EnsemblDatabaseReference

AT1G49500 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.13416266666667
p value: 9.32345106321237E-5
refers to: AT1G49500
refers to: 262399_at
t statistic: 15.7264819685749

257365_x_at
type: EFO_0005072
part of: A-AFFY-2

(Sample) organism/Arabidopsis thaliana
type: Arabidopsis thaliana
property type: organism
property value: Arabidopsis thaliana

SAG21
type: EnsemblDatabaseReference

TAT3 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.01196576666667
p value: 4.229178811983E-4
refers to: 263539_at
refers to: TAT3
t statistic: 12.2007043696973

PR4
type: EnsemblDatabaseReference

261131_at
type: EFO_0005072
part of: A-AFFY-2

AT1G49500
type: EnsemblDatabaseReference

pEARLI 1 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.01301276666667
p value: 0.0228713047083539
refers to: pEARLI 1
refers to: 254805_at
t statistic: 4.50628236419667

ATMYB90
type: EnsemblDatabaseReference

pEARLI 1
type: EnsemblDatabaseReference

PDF1.2b
type: EnsemblDatabaseReference

AT1G22060
type: EnsemblDatabaseReference

254805_at
type: EFO_0005072
part of: A-AFFY-2

AT1G65010
type: EnsemblDatabaseReference

TT7
type: EnsemblDatabaseReference

262871_at
type: EFO_0005072
part of: A-AFFY-2

255948_at
type: EFO_0005072
part of: A-AFFY-2

245393_at
type: EFO_0005072
part of: A-AFFY-2

260551_at
type: EFO_0005072
part of: A-AFFY-2

Analysis of 'des1-1 mutant' vs 'wild type'
type: MicroarrayDifferentialExpressionAnalysis

AT4G37080 DOWN in 'des1-1 mutant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.00736043333333
p value: 0.0282434037035613
refers to: AT4G37080
refers to: 246231_at
t statistic: -4.02107597404001

TAT3
type: EnsemblDatabaseReference

250558_at
type: EFO_0005072
part of: A-AFFY-2

HIS1-3
type: EnsemblDatabaseReference

250083_at
type: EFO_0005072
part of: A-AFFY-2

SEN1
type: EnsemblDatabaseReference

248291_at
type: EFO_0005072
part of: A-AFFY-2

GRP3S
type: EnsemblDatabaseReference

(Sample) genotype/wild type genotype
type: EFO_0000001
property type: genotype
property value: wild type genotype

AT1G22060 DOWN in 'des1-1 mutant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.1670937
p value: 0.0268893966827795
refers to: AT1G22060
refers to: 255948_at
t statistic: -4.12561065764653

AT5G53020
type: EnsemblDatabaseReference

256569_at
type: EFO_0005072
part of: A-AFFY-2

SAG21
type: EnsemblDatabaseReference

PR4
type: EnsemblDatabaseReference

(Assay) GSM476823
type: Assay
has participant: A-AFFY-2

AT3G19550
type: EnsemblDatabaseReference

258434_at
type: EFO_0005072
part of: A-AFFY-2

ATMYB90
type: EnsemblDatabaseReference

pEARLI 1
type: EnsemblDatabaseReference

AT4G16260 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 2.7167765
p value: 5.1274288054914E-5
refers to: AT4G16260
refers to: 245393_at
t statistic: 17.3265227480272

Arabidopsis thaliana
type: OBI_0100026

263046_at
type: EFO_0005072
part of: A-AFFY-2

FPP4 DOWN in 'des1-1 mutant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.0800133
p value: 0.033706204651154
refers to: FPP4
refers to: 261131_at
t statistic: -3.65446322982287

(Factor value) GENOTYPE/wild type genotype
type: EFO_0000001
property type: GENOTYPE
property value: wild type genotype

TT7 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.1073012
p value: 0.00421420555235943
refers to: 250558_at
refers to: TT7
t statistic: 7.82483647726283

ATGSTF12 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.00198733333333
p value: 0.00106496842775289
refers to: ATGSTF12
refers to: 250083_at
t statistic: 10.1790965148603

PR4 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.48651833333334
p value: 1.86948048918793E-4
refers to: PR4
refers to: 258791_at
t statistic: 14.200721875944

TT7
type: EnsemblDatabaseReference

(Assay) GSM476825
type: Assay
has participant: A-AFFY-2

HIS1-3 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.00012643333333
p value: 0.00106496842775289
refers to: HIS1-3
refers to: 265817_at
t statistic: 10.2169377874454

ATTI1
type: EnsemblDatabaseReference

AT1G65010 DOWN in 'des1-1 mutant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.10518686666667
p value: 0.0352235056285367
refers to: AT1G65010
refers to: 262871_at
t statistic: -3.57125851820462

AT3G19550 UP in 'des1-1 mutant' vs 'wild type'
type: IncreasedDifferentialExpressionRatio
fold change: 1.33501016666667
p value: 2.64984395872596E-4
refers to: AT3G19550
refers to: 256569_at
t statistic: 13.4038678442517

246302_at
type: EFO_0005072
part of: A-AFFY-2

CAX3
type: EnsemblDatabaseReference

TAT3
type: EnsemblDatabaseReference

263539_at
type: EFO_0005072
part of: A-AFFY-2

HIS1-3
type: EnsemblDatabaseReference

254832_at
type: EFO_0005072
part of: A-AFFY-2

AT5G53020
type: EnsemblDatabaseReference

AT5G53020 DOWN in 'des1-1 mutant' vs 'wild type'
type: DecreasedDifferentialExpressionRatio
fold change: -1.1092776
p value: 0.0263530509847078
refers to: AT5G53020
refers to: 248291_at
t statistic: -4.18114025581393

AT3G19550
type: EnsemblDatabaseReference

FPP4
type: EnsemblDatabaseReference

253161_at
type: EFO_0005072
part of: A-AFFY-2

(Assay) GSM476824
type: Assay
has participant: A-AFFY-2