Gnb1 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Pttg1 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Paip1 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
C130071C03Rik DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Ccdc127 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Adamts4 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
Zic1 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
Prune2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Analysis of 'dominant active mutant DREAM transgenic' vs 'wild type'
Egr2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Golt1b DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Prkaa2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Gfap DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Zfp386 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Nsg2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Tia1 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Tshz2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
4833420G17Rik DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Kcne2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Cbln1 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Slc5a5 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Mbd4 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Fam126b DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Csnk1a1 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Supt16 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Mndal UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
Kcnj9 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Rps3 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Camk4 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
4632427E13Rik DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Col3a1 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
Fos DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Npas4 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Zc2hc1a DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Dusp1 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Ssbp2 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
Mir1931 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Krt12 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Homer1 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
Changes in nuclear Ca2+ homeostasis activate specific gene expression programs and are central to the acquisition and the plastic storage of memories. DREAM /KChIP proteins form heterotetramers that bind DNA and repress transcription in a Ca2+-dependent manner. Single ablation of one member of the DREAM/KChIP family may result in a mild or the absence of phenotype due to partial gene compensation. To study the function of DREAM/KChIP proteins in the brain, we used transgenic mice expressing a Ca2+-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). We show that daDREAM controls the expression of several activity-dependent transcription factors including Npas4, Nr4a1, Mef2C, JunB and c-Fos, as well as the chromatin modifying enzyme Mbd4 and proteins related to actin polymerization like Arc and gelsolin. Thus, directly or through these targets, expression of daDREAM in the forebrain resulted in a complex phenotype characterized by i) impaired learning and memory, ii) loss of recurrent inhibition and enhanced LTP in the dentate gyrus without affecting Kv4-mediated potassium currents, and iii) modified spine density in DG granule neurons. Our results propose DREAM as a master-switch transcription factor regulating several activity-dependent gene expression programs to control synaptic plasticity, learning and memory. We used Affymetrix microarrays (GeneChip Mouse Genome 430 2.0) to compare global gene expression in wild type (WT) versus transgenic hippocampi (L26 mice). For ech type of sample three hybridizations were carried-out (independent biological replicates).
(Sample) genotype/dominant active mutant DREAM transgenic
Zfp939 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
(Factor value) GENOTYPE/dominant active mutant DREAM transgenic
Cdh6 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Adrbk2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Acer3 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Ang UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
Pisd-ps1 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
Arc DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Mtmr7 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Gm21967 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Nr4a1 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Plcxd2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Cd44 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Shoc2 DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Spink10 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'
4933439C10Rik DOWN in 'dominant active mutant DREAM transgenic' vs 'wild type'
Neat1 UP in 'dominant active mutant DREAM transgenic' vs 'wild type'