BETA-TIP DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
PIL6 DOWN in 'pif4; pif5' vs 'wild type' in 'dark'
AT3G49960 DOWN in 'pif4; pif5' vs 'wild type' in 'dark'
XTH7 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT4G13790 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
HFR1 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
Analysis of 'pif4; pif5' vs 'wild type' in 'dark'
POR C UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
COR414-TM1 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
ATOFP16 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT4G25260 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
POX2 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
MFP1 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT1G21520 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
COL2 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
LUP1 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT1G21500 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
ATEXLA2 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
4CL3 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
Analysis of 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
LP1 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT4G25250 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
PER59 DOWN in 'pif4; pif5' vs 'wild type' in 'dark'
AT1G12070 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
STR9 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT4G16670 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT2G34620 UP in 'pif4; pif5' vs 'wild type' in 'dark'
LHCB2.4 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
FAD4 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT2G34620 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
ATST2A DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
CKX5 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
MIOX4 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT4G35720 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT4G11610 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
XTR7 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT4G38860 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
ATFAO3 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
HMGB9 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT1G21500 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT3G05730 UP in 'pif4; pif5' vs 'wild type' in 'dark'
AT2G04500 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
PSBQ UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
CA1 UP in 'pif4; pif5' vs 'wild type' in 'dark'
PSAH2 UP in 'pif4; pif5' vs 'wild type' in 'dark'
AT1G77330 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT1G80130 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
LHCA1 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
ADS1 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
PLC7 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
PSAH2 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT1G29195 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AtHSD5 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
LHCB2.4 UP in 'pif4; pif5' vs 'wild type' in 'dark'
(Factor value) GROWTH_CONDITION/far-red light
AT1G30080 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT3G54510 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
PIL6 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
HIS1-3 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
LHCA1 UP in 'pif4; pif5' vs 'wild type' in 'dark'
AT2G34620 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
(Factor value) GENOTYPE/wild type genotype
AT1G29490 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
STR9 UP in 'pif4; pif5' vs 'wild type' in 'dark'
UGT72B2 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
ATST2B DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT5G18060 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
PGL5 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
MLP423 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
Analysis of 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT2G19970 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT1G78720 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
BEE1 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
Phytochromes are red/far red photosensors regulating numerous developmental programs in plants. Among them phytochrome A (phyA) is essential to enable seedling de-etiolation in continuous far-red (FR) light a condition mimicking the environment under a dense canopy. The ecological relevance of this response is demonstrated by the high mortality rate of phyA mutants germinating in deep vegetational shade. phyA signaling involves a direct interaction of the photoreceptor with members of the bHLH transcription factor family, PIF1 and PIF3 (Phytochrome Interacting Factor). Here we investigated the involvement of PIF4 and PIF5 in phyA signaling and found that they redundantly control de-etiolation in FR light. The pif4pif5 double mutant is hypersensitive to low fluence rates of FR light. This phenotype is dependent on FR light perception by phyA but does not rely on alterations of the phyA level. Our microarrays analysis shows that PIF4 and PIF5 are part of an inhibitory mechanism repressing the expression of some light-responsive genes in the dark and are also needed for full expression of several growth-related genes in the light. Unlike PIF1 and PIF3, PIF4 and PIF5 are not degraded in response to FR light indicating that they are light-regulated by a different mechanism. Our genetic analysis suggests that this is achieved through the sequestration of these PIFs by the closely related bHLH transcription factor HFR1 (long Hypocotyl in FR light). Experiment Overall Design: he pif4pif5 double mutant were compared to wild-type plants when kept in the dark or subjected to 1 or 24 hours of 0.5 or 5 ÃÂÃÂµmol/m2/s far-red light respectively.
CA1 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT3G19480 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
PTAC16 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT4G11210 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
PIL6 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT1G53490 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
GH3.9 UP in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
PAR1 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
AT1G43590 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '1 hour'
AT2G47780 DOWN in 'pif4; pif5' vs 'wild type' in 'far-red light' at '24 hour'
XTH7 UP in 'pif4; pif5' vs 'wild type' in 'dark'