KRT15 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NEFM DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
OAS1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
HAS3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SOCS3 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
LINC01279 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CAMP UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
IFIH1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NGF UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ABCA1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SCEL DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
IRF7 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NFE2L2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 6h, biological rep2
RWPE1 cells, vehicle, 6h, biological rep2
G6PD UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TAGLN DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MX1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SERPINB1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ARHGEF28 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 24h, biological rep1
RWPE1 cells, vehicle, 24h, biological rep1
IGFBP3 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CALML3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ARHGEF16 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
C2CD2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
BMP6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TGFB2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PTAFR UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CHRM3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SERPINE1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
LMCD1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PSMB8-AS1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
TCF7L2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
DENND6B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
PADI3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
APCDD1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SUSD2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CCDC80 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
FAM110C UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
IGFBP6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CXCL14 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
Analysis of '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
G6PD UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CA2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
ARHGEF37 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ABCA1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CCL2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
GCLC UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
FAM129A UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MAFB UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
DNER UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SULF1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
RASSF10 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
GRAMD4 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KLK6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CGN UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 24h, biological rep3
RWPE1 cells, vehicle, 24h, biological rep3
DDX58 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SOX7 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TMEM79 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
IFIT1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SERPINB13 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
DUSP10 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
Analysis of '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MME UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
RHOF UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CLMN UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CXCL14 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
MPV17L UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
ABCG1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TSPAN1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
AKAP12 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
LYPD5 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KAL1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 6h, biological rep1
RWPE1 cells, vehicle, 6h, biological rep1
TACSTD2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
IFIT3 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
BTBD11 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
PPP1R3C DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 24h, biological rep3
RWPE1 cells, 100 nM of 1,25(OH)2D, 24h, biological rep3
CALML3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CHST11 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KRT16 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MAPK13 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SPP1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SCEL DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
AKR1C3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MFAP5 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
FAM167A DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
PRICKLE1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MFI2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MALL UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 48h, biological rep2
RWPE1 cells, 100 nM of 1,25(OH)2D, 48h, biological rep2
THBD UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
FOXQ1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
ETS2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MFAP5 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
FOXQ1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KCNN4 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
AMOTL1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ZNF462 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
GEM UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ETNK2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
AKR1C3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CLDN1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CTSD UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
HRCT1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
DENND6B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
ABCA1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PLAU UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
FAM129A UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TNFSF15 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
FAT2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
EGR3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
EDN1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
STAT1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
PNLIPRP3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
FTH1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SULF1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TNS1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
GPCPD1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PTGES UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
ST6GALNAC5 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CST6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CD14 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
TMEM173 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
USP2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
PTGES UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Factor value) COMPOUND/1,25-dihydroxyvitamin D3
MARCH3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SYT7 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
TMEM40 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
RFFL UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PTGER4 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CDA UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SULF1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SEC14L1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TINAGL1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
WDR66 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
PADI3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
G0S2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SLC22A23 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CA9 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
S100A8 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NTM DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
Background: Prostate cancer is the second leading cause of cancer mortality among US men. Epidemiological evidence suggests that high vitamin D status protects men from prostate cancer and the active form of vitamin D, 1ÃÂÃÂ±,25 dihydroxyvitamin D3 (1,25(OH)2D) has anti-cancer effects in cultured prostate cells. Still, the molecular mechanisms and the gene targets for vitamin D-mediated prostate cancer prevention are unknown. Results: We examined the effect of 1,25(OH)2D (+/- 100 nM, 6, 24, 48 h) on the transcript profile of proliferating RWPE1 cells, an immortalized, non-tumorigenic prostate epithelial cell line that is growth arrested by 1,25(OH)2D (Affymetrix U133 Plus 2.0, n=4/treatment per time and dose). Our analysis revealed many transcript level changes at a 5% false detection rate: 6 h, 1571 (61% up), 24 h, 1816 (60 % up), 48 h, 3566 (38 % up). 288 transcripts were regulated similarly at all time points (182 up, 80 down) and many of the promoters for these transcripts contained putative vitamin D response elements. Functional analysis by pathway or Gene Set Analysis revealed early suppression of WNT, Notch, NF-kB, and IGF1 signaling. Transcripts related to inflammation were suppressed at 6 h (e.g. IL-1 pathway) and suppression of proinflammatory pathways continued at later time points (e.g. IL-17 and IL-6 pathways). There was also evidence for induction of anti-angiogenic pathways and induction of transcripts for protection from oxidative stress or maintenance of cell redox homeostasis at 6 h. Conclusions: Our data reveal of large number of potential new, direct vitamin D target genes relevant to prostate cancer prevention. In addition, our data suggests that rather than having a single strong regulatory effect, vitamin D orchestrates a pattern of changes within prostate epithelial cells that limit or slow carcinogenesis. Experiment Overall Design: RWPE1 cells were treated with medium containing 100 nM of 1,25(OH)2D or vehicle (0.1% ethanol) for 6, 24 or 48 hours (n=4 per treatment, 24 total samples). The transcripts levels in each sample were measured by using the Affymetrix HU133 plus 2.0 GeneChip (Affymetrix, Santa Clara, CA).
MED24 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
RASSF5 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
LOX UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
P2RY2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
FLRT2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TRIM6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PDPN UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
F2R DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SLC45A4 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ZFP36 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KLF4 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TMEM40 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
GDPD3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
DHRS9 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ISG15 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
KLK5 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
HBEGF UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MMP2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
PLAT UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PADI3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CYSTM1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
FHOD3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 24h, biological rep2
RWPE1 cells, 100 nM of 1,25(OH)2D, 24h, biological rep2
IFI6 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
ETNK2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CDA UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
SGPP2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
KIAA1324L UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CYP24A1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SGPP2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
DENND6B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
FOS UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
TMEM40 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CCL2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
FOXQ1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
EDN1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
HBEGF UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
HES1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PLD6 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
RGCC UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SEMA3B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
BST2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
ADIRF UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
HERC6 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CRLF1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
KLK6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
G6PD UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CST6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TFCP2L1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 6h, biological rep2
RWPE1 cells, 100 nM of 1,25(OH)2D, 6h, biological rep2
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 6h, biological rep4
RWPE1 cells, 100 nM of 1,25(OH)2D, 6h, biological rep4
CD14 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
ARSI UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PRR16 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CLDN23 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 48h, biological rep1
RWPE1 cells, vehicle, 48h, biological rep1
OAS2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CYP24A1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 6h, biological rep1
RWPE1 cells, 100 nM of 1,25(OH)2D, 6h, biological rep1
ADAMTS15 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KAL1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MX1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
MTSS1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
ITGB6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KLK5 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
PCDH7 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
FST DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TXNRD1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
NET1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
NPTX2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
(Assay) RWPE1 cells, vehicle, 48h, biological rep2
RWPE1 cells, vehicle, 48h, biological rep2
IFI27 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CRLF1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
BTBD11 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
DUSP6 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PDE2A UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KIF26A UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TRIM22 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
USP2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PRSS23 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
FTH1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
NINJ1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SEMA3B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NFKBIA UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KRT6B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NGEF UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NTM DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
AP001053.11 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SLC44A5 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
APCDD1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
FBLIM1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 6h, biological rep3
RWPE1 cells, 100 nM of 1,25(OH)2D, 6h, biological rep3
NRP1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MEX3B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SPINK6 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CYP24A1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
THBD UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MED24 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
FIBIN DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
EFNB2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 48h, biological rep1
RWPE1 cells, 100 nM of 1,25(OH)2D, 48h, biological rep1
FOXK1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
LRIG3 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MED24 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SNRK UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 24h, biological rep2
RWPE1 cells, vehicle, 24h, biological rep2
CYP1A1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
C1S DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
EPHB6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
IL1A DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
DDX60 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NANOS1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
EFTUD1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PARP14 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
IGFBP3 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SERPINB2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SEMA3B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
AFAP1L2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PARP9 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
IER5L UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 6h, biological rep3
RWPE1 cells, vehicle, 6h, biological rep3
KIT UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
BTBD11 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KCNJ2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
BCL11B DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MRGPRX3 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
FAM43A UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 48h, biological rep4
RWPE1 cells, 100 nM of 1,25(OH)2D, 48h, biological rep4
KRT15 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
AP001053.11 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
LINC01279 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
RP11-21L23.2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KRT6B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
IGFBP3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 48h, biological rep4
RWPE1 cells, vehicle, 48h, biological rep4
MRAP2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 24h, biological rep4
RWPE1 cells, 100 nM of 1,25(OH)2D, 24h, biological rep4
CRLF1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PITPNC1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
IGFBP6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
ADSSL1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CD97 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CITED4 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SHE UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CCDC80 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
IFI44L DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 24h, biological rep1
RWPE1 cells, 100 nM of 1,25(OH)2D, 24h, biological rep1
CD274 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CDA UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SPRR1B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
XAF1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SCNN1G UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
GATA2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MMP28 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
HLF DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
KRT13 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
DTX3L DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
TPST1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
IFNK DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SERPINB2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
GATA6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SLC46A1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PCDH7 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
(Assay) RWPE1 cells, vehicle, 24h, biological rep4
RWPE1 cells, vehicle, 24h, biological rep4
SEMA6D UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CYP26B1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
FOS UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PDE2A UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
TSKU UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PHACTR3 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SOSTDC1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SPRR1B UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
SAMHD1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
IFITM1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
LYPD3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MATN2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
ARL4D UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CST6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
PTGES UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
MN1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
LAMB3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, 100 nM of 1,25(OH)2D, 48h, biological rep3
RWPE1 cells, 100 nM of 1,25(OH)2D, 48h, biological rep3
CLDN11 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CA9 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
TSPAN1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
EDIL3 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CA2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
RNF24 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
(Assay) RWPE1 cells, vehicle, 48h, biological rep3
RWPE1 cells, vehicle, 48h, biological rep3
EPSTI1 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
PTGS2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
IFI35 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
KLK10 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
CTGF DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CD14 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
PDE2A UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
IFI44 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
ETNK2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
DNER UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
PSMB9 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
NPTX2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
GAS6 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
KLK6 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
CMPK2 DOWN in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
(Assay) RWPE1 cells, vehicle, 6h, biological rep4
RWPE1 cells, vehicle, 6h, biological rep4
PTPN1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TMEM37 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
Analysis of '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
SLC4A7 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
TSLP UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MFI2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'
CALML3 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
TNFAIP2 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '6 hour'
MTSS1 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '48 hour'
KRT16 UP in '1,25-dihydroxyvitamin D3' vs 'none' at '24 hour'