Analysis of 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Sectm1a UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
(Factor value) CELL_TYPE/Lineage-c-Kit+Sca-1+CD34+
Sgip1 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Stag3 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Car3 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Tex19.2 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Dpep3 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
(Factor value) CELL_TYPE/Lineage-c-Kit+Sca-1-
Ccrn4l UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
DNA methylation is essential for mammalian development and plays crucial roles in a variety of biological processes. The DNA methyltransferase Dnmt1 serves to maintain parental cell methylation patterns on daughter DNA strands in mitotic cells, however, the precise role of Dnmt1 in regulation of quiescent adult stem cells is not known. To examine the role of Dnmt1 in adult hematopoietic stem cells (HSCs), we conditionally disrupted Dnmt1 in the hematopoietic system. We used microarrays to profile the global gene expression program in hematopoietic stem and progenitor cells following deletion of Dnmt1. Dnmt1 was conditionally deleted in the hematopoietic system by injections of poly(I)poly(C) to induce Cre expression from the Mx-Cre transgene. Control mice were also injected with poly(I)poly(C) but do not carry the Mx-Cre transgene. Four days after completion of poly(I)poly(C) injections, bone marrow was harvested from the mice, antibody-mediated magnetic bead selection was used to remove cells expressing mature lineage markers, and the resulting lineage-depleted cells were stained with fluorochrome-conjugated antibodies against the surface receptors c-Kit, Sca-1 and CD34. Populations of LT-HSCs, MPPs and myeloid progenitors were FACS sorted, RNA was extracted and amplified from these sorted populations and hybridized to Affymetrix microarrays to compare changes in gene expression induced by conditional knockout of Dnmt1 compared to control in each of the three cell populations. There are 2 biological replicates for the LT-HSCs and MPPs and 3 biological replicates for the myeloid progenitors.
Inadl UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Hamp2 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Fkbp6 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Tnnt1 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Slc4a8 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Dazl UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
2010005H15Rik UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Rpl39l UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Nxf2 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Tex12 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Tex19.1 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
(Factor value) GENOTYPE/Dnmt1 fl/fl Mx-Cre
(Sample) cell type/Lineage-c-Kit+Sca-1+CD34+
Pard6b UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Brdt UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Clec11a UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Ipp UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
(Sample) cell type/Lineage-c-Kit+Sca-1+CD34-
Strc UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Trap1a UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Nrg4 UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
Rps4l UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'
(Factor value) CELL_TYPE/Lineage-c-Kit+Sca-1+CD34-
4930550L24Rik UP in 'Dnmt1 fl/fl Mx-Cre' vs 'Dnmt1 fl/fl' in 'Lineage-c-Kit+Sca-1-'