UNE8 UP in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT4G16260 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATCSLA01 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
(Factor value) GENOTYPE/wild type genotype
AT4G11190 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G15010 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
LSU1 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATHCHIB DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
The Mediator complex is an evolutionary conserved multiprotein complex that plays an essential role in initiating and regulating transcription. Its function is to act as a universal adaptor between RNA Polymerase II and DNA-bound transcription factors to translate regulatory information from activators and repressors to the transcriptional machinery. We have found that the PFT1 gene (which encodes the MED25 subunit of the Mediator complex) is required for the uncompromised expression of both salicylic acid- and jasmonate-dependent defense genes as well as resistance to the leaf-infecting fungal pathogens, Alternaria brassicicola and Botrytis cinerea in Arabidopsis. Surprisingly, we found that the pft1/med25 mutant showed increased resistance to the root infecting pathogen Fusarium oxysporum and that this resistance was independent of classical defense genes. In addition, the over-expression of PFT1 led to increased susceptibility to F. oxysporum. Therefore, to explore this phenomenon further, we wished to use whole genome transcript profiling to identify which genes may be playing a role in pft1/med25-mediated resistance to F. oxysporum. Experiment Overall Design: We grew both WT and pft1/med25 plants for four weeks in soil. After four weeks we treated the plants by root-dipping in either water or a F. oxysporum spore suspension before replanting them back into soil. There were four independent replicates of each treatment and each replicate contained 20 plants each. The treatments were WT (mock): pft1 (mock): WT (infected): pft1 (infected). Each replicate (16 in total) was harvested after 48 hours and the resulting RNA was used for hybridization to an Affymetrix ATH1 chip.
ZIP5 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
CYP86A7 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT4G29700 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
LAL5 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
MEE3 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
ATXTH19 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AtRLP19 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
STZ DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT3G16530 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT3G16450 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT3G05730 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
GH3.3 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT2G39030/T7F6.20 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
JMT DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
PER39 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT4G08300 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT4G17470 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
QQS UP in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT2G13820 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AKR4C8 UP in 'Fusarium oxysporum' vs 'none' in 'wild type'
ORA59 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G33340 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
QQS UP in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
CYP81F4 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
WRKY54 UP in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
(Factor value) INFECT/Fusarium oxysporum
AT4G01390 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
PR4 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
MAM-L DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
PFT1 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATCLH1 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
GLR5 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATEXT4 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
MAM-L DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
GASA9 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT1G54020 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATCLH1 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATEXT4 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
PNP-A UP in 'Fusarium oxysporum' vs 'none' in 'wild type'
UBC17 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G62510 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT1G54020 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
ATMPK3 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AtRLP19 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
ATSDI1 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
SD11 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT3G28220 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
Analysis of 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G66100 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
PNP-A UP in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
CYP86A4 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
OMTF3 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATMRU1 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT1G66100 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
HSPRO2 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
LTP2 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATDR4 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
SUFE2 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
LTP2 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT1G62510 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT5G03350 UP in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
SD11 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
GRXC11 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
PDF1.2b DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
Analysis of 'Fusarium oxysporum' vs 'none' in 'wild type'
AZI1 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT2G13820 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT2G43590 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
CYP71B22 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
BXL5 UP in 'Fusarium oxysporum' vs 'none' in 'wild type'
JAZ9 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G31710 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT3G16670 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATMRU1 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
DIN11 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
PFT1 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT4G01390 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G31690 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT3G09020 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G21100 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
CYP86A4 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
PER21 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT2G34810 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ZIP5 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
PR4 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
ATPRP2 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
CYP81F4 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G31690 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT4G25780 UP in 'Fusarium oxysporum' vs 'none' in 'wild type'
WRKY40 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT4G24350 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'
AT1G21100 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
AT4G24350 DOWN in 'Fusarium oxysporum' vs 'none' in 'wild type'
ATEXLB1 DOWN in 'Fusarium oxysporum' vs 'none' in 'pft1 mutant'