Aldoc DOWN in 'Hunk knockout' vs 'wild type genotype'
1810011O10Rik UP in 'Hunk knockout' vs 'wild type genotype'
Akap12 DOWN in 'Hunk knockout' vs 'wild type genotype'
Kcnn4 DOWN in 'Hunk knockout' vs 'wild type genotype'
Analysis of 'Hunk knockout' vs 'wild type genotype'
Sfrp2 UP in 'Hunk knockout' vs 'wild type genotype'
C3 UP in 'Hunk knockout' vs 'wild type genotype'
Slc28a3 UP in 'Hunk knockout' vs 'wild type genotype'
Nrep UP in 'Hunk knockout' vs 'wild type genotype'
Pip UP in 'Hunk knockout' vs 'wild type genotype'
Add3 UP in 'Hunk knockout' vs 'wild type genotype'
Evl DOWN in 'Hunk knockout' vs 'wild type genotype'
Hspa1b UP in 'Hunk knockout' vs 'wild type genotype'
Kit DOWN in 'Hunk knockout' vs 'wild type genotype'
Rhpn2 UP in 'Hunk knockout' vs 'wild type genotype'
Pgd UP in 'Hunk knockout' vs 'wild type genotype'
Ccl28 UP in 'Hunk knockout' vs 'wild type genotype'
Npnt DOWN in 'Hunk knockout' vs 'wild type genotype'
Sgk1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Anxa1 UP in 'Hunk knockout' vs 'wild type genotype'
Duoxa1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Irs1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Errfi1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Fblim1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Chpt1 UP in 'Hunk knockout' vs 'wild type genotype'
Tmem47 DOWN in 'Hunk knockout' vs 'wild type genotype'
Bex2 DOWN in 'Hunk knockout' vs 'wild type genotype'
Sema3b DOWN in 'Hunk knockout' vs 'wild type genotype'
Emid1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Abhd5 UP in 'Hunk knockout' vs 'wild type genotype'
Gadd45g UP in 'Hunk knockout' vs 'wild type genotype'
Thrsp UP in 'Hunk knockout' vs 'wild type genotype'
Socs2 DOWN in 'Hunk knockout' vs 'wild type genotype'
Rnf128 UP in 'Hunk knockout' vs 'wild type genotype'
Ckmt1 UP in 'Hunk knockout' vs 'wild type genotype'
Fgfr1 DOWN in 'Hunk knockout' vs 'wild type genotype'
(Factor value) GENOTYPE/wild type genotype
2610018G03Rik DOWN in 'Hunk knockout' vs 'wild type genotype'
Tmem30b UP in 'Hunk knockout' vs 'wild type genotype'
Pros1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Cpd DOWN in 'Hunk knockout' vs 'wild type genotype'
4931406C07Rik DOWN in 'Hunk knockout' vs 'wild type genotype'
Krt19 UP in 'Hunk knockout' vs 'wild type genotype'
G0s2 UP in 'Hunk knockout' vs 'wild type genotype'
Cryab UP in 'Hunk knockout' vs 'wild type genotype'
Chdh UP in 'Hunk knockout' vs 'wild type genotype'
Dcxr UP in 'Hunk knockout' vs 'wild type genotype'
Dusp6 DOWN in 'Hunk knockout' vs 'wild type genotype'
Cytip UP in 'Hunk knockout' vs 'wild type genotype'
Glul UP in 'Hunk knockout' vs 'wild type genotype'
St6galnac4 DOWN in 'Hunk knockout' vs 'wild type genotype'
Acaa2 UP in 'Hunk knockout' vs 'wild type genotype'
Cyp2f2 UP in 'Hunk knockout' vs 'wild type genotype'
Bid DOWN in 'Hunk knockout' vs 'wild type genotype'
Fam174b DOWN in 'Hunk knockout' vs 'wild type genotype'
Col9a1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Eya2 UP in 'Hunk knockout' vs 'wild type genotype'
Selenbp1 UP in 'Hunk knockout' vs 'wild type genotype'
Grb10 UP in 'Hunk knockout' vs 'wild type genotype'
Nfib DOWN in 'Hunk knockout' vs 'wild type genotype'
We previously identified a novel SNF1/AMPK-related protein kinase, Hunk, from a mammary tumor arising in an MMTV-neu transgenic mouse. The function of this kinase is unknown. Using targeted deletion in mice, we now demonstrate that Hunk is required for the metastasis of c-myc-induced mammary tumors, but is dispensable for normal development. Reconstitution experiments revealed that Hunk is sufficient to restore the metastatic potential of Hunk-deficient tumor cells, as well as defects in migration and invasion, and does so in a manner that requires its kinase activity. Consistent with a role for Hunk in the progression of human cancers, the human homologue of Hunk is overexpressed in aggressive subsets of carcinomas of the ovary, colon, and breast. In addition, a murine gene expression signature that distinguishes Hunk-wild type from Hunk-deficient mammary tumors predicts clinical outcome in women with breast cancer. Together, these findings establish a role for Hunk in metastasis and an in vivo function for this kinase. Hunk-deficient animals were crossed to mice harboring an MMTV-c-myc transgene (Leder et al., 1986). Hunk heterozygous, MMTV-c-myc mice were backcrossed to Hunk heterozygous animals. MMTV-c-myc female animals of each Hunk genotype were mated twice, then monitored twice weekly for mammary tumors. Mice possessing tumors with a maximum diameter of 20 mm were sacrificed and organs were examined at necropsy. Tumor nodules were identified by examination of organs through a Leica Wild MZ8 dissection microscope.
Lrg1 UP in 'Hunk knockout' vs 'wild type genotype'
Ddit4 UP in 'Hunk knockout' vs 'wild type genotype'
Chn2 DOWN in 'Hunk knockout' vs 'wild type genotype'
Shf DOWN in 'Hunk knockout' vs 'wild type genotype'
Ctbp2 DOWN in 'Hunk knockout' vs 'wild type genotype'
Serpine2 DOWN in 'Hunk knockout' vs 'wild type genotype'
Fam3c DOWN in 'Hunk knockout' vs 'wild type genotype'
Ltf UP in 'Hunk knockout' vs 'wild type genotype'
Deptor DOWN in 'Hunk knockout' vs 'wild type genotype'
Pax6 UP in 'Hunk knockout' vs 'wild type genotype'
Slc6a15 DOWN in 'Hunk knockout' vs 'wild type genotype'
Plet1 UP in 'Hunk knockout' vs 'wild type genotype'
Spry2 DOWN in 'Hunk knockout' vs 'wild type genotype'
Pik3r1 UP in 'Hunk knockout' vs 'wild type genotype'
Krt79 UP in 'Hunk knockout' vs 'wild type genotype'
Tfcp2l1 UP in 'Hunk knockout' vs 'wild type genotype'
Hp UP in 'Hunk knockout' vs 'wild type genotype'
Elf5 UP in 'Hunk knockout' vs 'wild type genotype'
Bex1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Bdh1 UP in 'Hunk knockout' vs 'wild type genotype'
Rbp7 DOWN in 'Hunk knockout' vs 'wild type genotype'
Nupr1 UP in 'Hunk knockout' vs 'wild type genotype'
Prom1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Cbr2 UP in 'Hunk knockout' vs 'wild type genotype'
Nudt4 DOWN in 'Hunk knockout' vs 'wild type genotype'
Pigr UP in 'Hunk knockout' vs 'wild type genotype'
Etv5 DOWN in 'Hunk knockout' vs 'wild type genotype'
Tceal8 DOWN in 'Hunk knockout' vs 'wild type genotype'
Nr4a1 DOWN in 'Hunk knockout' vs 'wild type genotype'
Tm4sf1 DOWN in 'Hunk knockout' vs 'wild type genotype'