PLEKHA2 DOWN in 'CD3 and CD28' vs 'none'
ARHGDIB DOWN in 'CD3 and CD28' vs 'none'
RP11-747H7.3 DOWN in 'CD3 and CD28' vs 'none'
TRABD2A DOWN in 'CD3 and CD28' vs 'none'
DENND4C DOWN in 'CD3 and CD28' vs 'none'
LDLRAP1 DOWN in 'CD3 and CD28' vs 'none'
PLEKHA1 DOWN in 'CD3 and CD28' vs 'none'
GS1-358P8.4 DOWN in 'CD3 and CD28' vs 'none'
FAM117A DOWN in 'CD3 and CD28' vs 'none'
CDC42SE1 DOWN in 'CD3 and CD28' vs 'none'
ARHGAP15 DOWN in 'CD3 and CD28' vs 'none'
RP11-277P12.20 UP in 'CD3 and CD28' vs 'none'
FAM105A DOWN in 'CD3 and CD28' vs 'none'
ST3GAL5 DOWN in 'CD3 and CD28' vs 'none'
RASGRP2 DOWN in 'CD3 and CD28' vs 'none'
UBASH3A DOWN in 'CD3 and CD28' vs 'none'
C16orf54 DOWN in 'CD3 and CD28' vs 'none'
RP11-303E16.2 UP in 'CD3 and CD28' vs 'none'
PITPNC1 DOWN in 'CD3 and CD28' vs 'none'
HERPUD2 DOWN in 'CD3 and CD28' vs 'none'
SH3BGRL DOWN in 'CD3 and CD28' vs 'none'
RP11-139J15.2 DOWN in 'CD3 and CD28' vs 'none'
RNF144B DOWN in 'CD3 and CD28' vs 'none'
FAM111A DOWN in 'CD3 and CD28' vs 'none'
DLGAP1-AS1 DOWN in 'CD3 and CD28' vs 'none'
GLTSCR1L DOWN in 'CD3 and CD28' vs 'none'
RABGAP1L DOWN in 'nitric oxide' vs 'none'
C11orf21 DOWN in 'CD3 and CD28' vs 'none'
KIAA0319L DOWN in 'CD3 and CD28' vs 'none'
PDCD4-AS1 DOWN in 'CD3 and CD28' vs 'none'
CD3-positive T cells were negatively isolated from 10 SLE patients and 9 healthy controls without SLE. All of the SLE samples and control samples were compared with one another to identify baseline differences in expression due to the disease. Next, T cell preparations from 4 of the control subjects were stimulated with either Nitric Oxide (NOC-18) 600 uM for 24hr or stimulated through CD3/CD28 for 24hr to determine which genes were responsive to these signaling mechanisms. Here, we show that activity of the mammalian target of rapamycin (mTOR), which is a sensor of the mitochondrial transmembrane potential, is increased in SLE T cells. Activation of mTOR was inducible by NO, a key trigger of MHP which in turn enhanced the expression of HRES-1/Rab4, a small GTPase that regulates recycling of surface receptors through early endosomes. Expression of HRES-1/Rab4 was increased in SLE T cells and, in accordance with its dominant impact on the endocytic recycling of CD4, it was inversely correlated with diminished CD4 expression. HRES-1/Rab4 over-expression was also inversely correlated with diminished TCRÃÂÃÂ¶ protein levels. Combined with follow up studies, these results suggest that activation of mTOR causes the loss of TCRÃÂÃÂ¶ in lupus T cells through HRES-1/Rab4-dependent lysosomal degradation. Experiment Overall Design: 10 replicate T cell samples from SLE (Lupus) patients Experiment Overall Design: 9 replicate T cell samples from healthy control (BC) subjects Experiment Overall Design: 4 replicate Nitric Oxide (NOC-18) stimulated T cell samples from 4 of the control subjects Experiment Overall Design: 4 replicate CD3/CD28 stimulated T cell samples from 4 of the control subjects
LEF1-AS1 DOWN in 'CD3 and CD28' vs 'none'
PPP2R5C DOWN in 'CD3 and CD28' vs 'none'
NFATC2IP DOWN in 'CD3 and CD28' vs 'none'
GABPB1-AS1 DOWN in 'CD3 and CD28' vs 'none'
RP11-390P24.1 DOWN in 'CD3 and CD28' vs 'none'
WHSC1L1 DOWN in 'CD3 and CD28' vs 'none'
PIK3IP1 DOWN in 'CD3 and CD28' vs 'none'
NGFRAP1 DOWN in 'CD3 and CD28' vs 'none'
TMEM63A DOWN in 'CD3 and CD28' vs 'none'
TCP11L2 DOWN in 'CD3 and CD28' vs 'none'
C3orf58 DOWN in 'CD3 and CD28' vs 'none'
TNFAIP8L1 DOWN in 'CD3 and CD28' vs 'none'
RABGAP1L DOWN in 'CD3 and CD28' vs 'none'
ALDH6A1 DOWN in 'CD3 and CD28' vs 'none'
SLC20A1 DOWN in 'CD3 and CD28' vs 'none'
HLA-DOA DOWN in 'CD3 and CD28' vs 'none'
PTPLAD2 DOWN in 'CD3 and CD28' vs 'none'
ZCCHC11 DOWN in 'CD3 and CD28' vs 'none'
RNF157-AS1 DOWN in 'CD3 and CD28' vs 'none'
FLJ31306 DOWN in 'CD3 and CD28' vs 'none'
ZFYVE20 DOWN in 'CD3 and CD28' vs 'none'
HIST1H2AC DOWN in 'CD3 and CD28' vs 'none'
ATF7IP2 DOWN in 'CD3 and CD28' vs 'none'
PLEKHB1 DOWN in 'CD3 and CD28' vs 'none'
CBFA2T2 DOWN in 'CD3 and CD28' vs 'none'
AKAP17A DOWN in 'CD3 and CD28' vs 'none'
TNFRSF10B UP in 'nitric oxide' vs 'none'
ST3GAL1 DOWN in 'CD3 and CD28' vs 'none'
RP11-159N11.4 DOWN in 'CD3 and CD28' vs 'none'
RP5-1024G6.8 UP in 'CD3 and CD28' vs 'none'
SLC35E2B DOWN in 'CD3 and CD28' vs 'none'
RP4-717I23.3 DOWN in 'CD3 and CD28' vs 'none'
TP53INP1 DOWN in 'CD3 and CD28' vs 'none'
GATAD2B DOWN in 'CD3 and CD28' vs 'none'
RP11-553L6.5 DOWN in 'CD3 and CD28' vs 'none'
FAM102A DOWN in 'CD3 and CD28' vs 'none'
RASGRF2 DOWN in 'nitric oxide' vs 'none'
RAB11FIP1 UP in 'CD3 and CD28' vs 'none'
RP11-23P13.6 DOWN in 'CD3 and CD28' vs 'none'
SYNCRIP DOWN in 'nitric oxide' vs 'none'
CACNA1I DOWN in 'CD3 and CD28' vs 'none'
TSPAN32 DOWN in 'CD3 and CD28' vs 'none'
LINC00116 UP in 'CD3 and CD28' vs 'none'
RP11-326G21.1 DOWN in 'CD3 and CD28' vs 'none'
DKFZP586I1420 DOWN in 'CD3 and CD28' vs 'none'
GABARAPL1 DOWN in 'CD3 and CD28' vs 'none'
FAM172A DOWN in 'nitric oxide' vs 'none'
KIAA0430 DOWN in 'CD3 and CD28' vs 'none'
RP5-935K16.1 UP in 'CD3 and CD28' vs 'none'
KLF3-AS1 DOWN in 'CD3 and CD28' vs 'none'
AL450992.2 UP in 'CD3 and CD28' vs 'none'
TNFSF13B DOWN in 'CD3 and CD28' vs 'none'
MAPK1IP1L UP in 'CD3 and CD28' vs 'none'
TMEM245 DOWN in 'CD3 and CD28' vs 'none'
FAM214A DOWN in 'CD3 and CD28' vs 'none'
KIAA1551 DOWN in 'CD3 and CD28' vs 'none'
RP11-383J24.6 DOWN in 'CD3 and CD28' vs 'none'
SLC46A3 DOWN in 'CD3 and CD28' vs 'none'
RP11-283I3.6 DOWN in 'CD3 and CD28' vs 'none'
TOPORS-AS1 DOWN in 'CD3 and CD28' vs 'none'
RP11-539L10.2 DOWN in 'CD3 and CD28' vs 'none'
LEPROTL1 DOWN in 'CD3 and CD28' vs 'none'
TRAF3IP3 DOWN in 'CD3 and CD28' vs 'none'
THEMIS2 DOWN in 'CD3 and CD28' vs 'none'
C14orf28 DOWN in 'CD3 and CD28' vs 'none'
TSC22D3 DOWN in 'CD3 and CD28' vs 'none'
S100PBP DOWN in 'CD3 and CD28' vs 'none'
ZFP36L2 DOWN in 'CD3 and CD28' vs 'none'
AC013394.2 DOWN in 'CD3 and CD28' vs 'none'
KIAA0355 DOWN in 'CD3 and CD28' vs 'none'
HNRNPU-AS1 DOWN in 'CD3 and CD28' vs 'none'
GPRASP1 DOWN in 'CD3 and CD28' vs 'none'
C10orf128 UP in 'CD3 and CD28' vs 'none'
SLC44A2 DOWN in 'CD3 and CD28' vs 'none'
PPP2R1B DOWN in 'nitric oxide' vs 'none'
LINC00674 DOWN in 'CD3 and CD28' vs 'none'
CYSLTR1 DOWN in 'CD3 and CD28' vs 'none'
RP4-581F12.1 UP in 'CD3 and CD28' vs 'none'
KIAA1109 DOWN in 'CD3 and CD28' vs 'none'
ZSCAN18 DOWN in 'CD3 and CD28' vs 'none'
CDK5RAP2 DOWN in 'CD3 and CD28' vs 'none'
RP11-498C9.15 UP in 'CD3 and CD28' vs 'none'
RARRES3 DOWN in 'CD3 and CD28' vs 'none'
TMEM50B DOWN in 'CD3 and CD28' vs 'none'
UHRF1BP1L UP in 'CD3 and CD28' vs 'none'
RP11-489E7.4 DOWN in 'CD3 and CD28' vs 'none'
HIST1H1C DOWN in 'CD3 and CD28' vs 'none'
TMEM116 DOWN in 'CD3 and CD28' vs 'none'
RP11-1399P15.1 DOWN in 'CD3 and CD28' vs 'none'
HNRNPA2B1 DOWN in 'CD3 and CD28' vs 'none'
AP001258.4 DOWN in 'CD3 and CD28' vs 'none'
ZFYVE16 DOWN in 'CD3 and CD28' vs 'none'
CCDC88C DOWN in 'CD3 and CD28' vs 'none'
LDLRAD4 DOWN in 'CD3 and CD28' vs 'none'
ARHGAP9 DOWN in 'CD3 and CD28' vs 'none'
RP11-147L13.15 DOWN in 'CD3 and CD28' vs 'none'
SLC2A4RG DOWN in 'CD3 and CD28' vs 'none'
RP11-172H24.4 UP in 'CD3 and CD28' vs 'none'
C2orf68 DOWN in 'CD3 and CD28' vs 'none'
LINC00324 DOWN in 'CD3 and CD28' vs 'none'
RP11-617F23.1 DOWN in 'CD3 and CD28' vs 'none'
XXbac-BPG181B23.7 DOWN in 'CD3 and CD28' vs 'none'
FAM200B DOWN in 'CD3 and CD28' vs 'none'
HNRNPA3 DOWN in 'nitric oxide' vs 'none'
FAM199X DOWN in 'CD3 and CD28' vs 'none'
TBC1D15 DOWN in 'CD3 and CD28' vs 'none'
RP11-285F7.2 DOWN in 'CD3 and CD28' vs 'none'
C1orf162 DOWN in 'CD3 and CD28' vs 'none'
FAM134B DOWN in 'CD3 and CD28' vs 'none'
RNASEH1-AS1 UP in 'CD3 and CD28' vs 'none'
CTB-31O20.2 DOWN in 'CD3 and CD28' vs 'none'
GOLGA8A DOWN in 'CD3 and CD28' vs 'none'
AC005523.2 UP in 'CD3 and CD28' vs 'none'
FAM118A DOWN in 'CD3 and CD28' vs 'none'
CSNK1G2 DOWN in 'CD3 and CD28' vs 'none'
HSD17B11 DOWN in 'CD3 and CD28' vs 'none'
RASGRP1 DOWN in 'CD3 and CD28' vs 'none'
SYNJ2BP DOWN in 'CD3 and CD28' vs 'none'
FAM210B DOWN in 'CD3 and CD28' vs 'none'
TNRC6C-AS1 DOWN in 'CD3 and CD28' vs 'none'
PPP1R3E DOWN in 'CD3 and CD28' vs 'none'
ITGB2-AS1 DOWN in 'CD3 and CD28' vs 'none'
RASGRF2 DOWN in 'CD3 and CD28' vs 'none'
FAM117B DOWN in 'CD3 and CD28' vs 'none'
ARL6IP5 DOWN in 'CD3 and CD28' vs 'none'
TMEM107 DOWN in 'CD3 and CD28' vs 'none'
CSGALNACT1 DOWN in 'CD3 and CD28' vs 'none'
ZCCHC18 DOWN in 'CD3 and CD28' vs 'none'
SLC22A23 DOWN in 'CD3 and CD28' vs 'none'
RP11-18H21.1 DOWN in 'CD3 and CD28' vs 'none'
CXorf65 DOWN in 'CD3 and CD28' vs 'none'
TMEM204 DOWN in 'CD3 and CD28' vs 'none'
SLC20A1 DOWN in 'nitric oxide' vs 'none'
RP11-553L6.5 DOWN in 'nitric oxide' vs 'none'
RALGPS2 DOWN in 'CD3 and CD28' vs 'none'
GLTSCR2 DOWN in 'CD3 and CD28' vs 'none'
DENND6A DOWN in 'CD3 and CD28' vs 'none'
PDE4DIP DOWN in 'CD3 and CD28' vs 'none'
METTL7A DOWN in 'CD3 and CD28' vs 'none'
SLC40A1 DOWN in 'CD3 and CD28' vs 'none'
DENND6A DOWN in 'nitric oxide' vs 'none'
RP11-448G15.3 DOWN in 'CD3 and CD28' vs 'none'
LINC00528 DOWN in 'CD3 and CD28' vs 'none'
RP11-196G11.5 DOWN in 'nitric oxide' vs 'none'
N4BP2L2 DOWN in 'nitric oxide' vs 'none'
SLC26A11 DOWN in 'CD3 and CD28' vs 'none'
RP11-67L2.2 DOWN in 'CD3 and CD28' vs 'none'
C14orf64 DOWN in 'CD3 and CD28' vs 'none'
C1orf21 DOWN in 'CD3 and CD28' vs 'none'
SERINC3 DOWN in 'CD3 and CD28' vs 'none'
RP11-707M3.3 DOWN in 'CD3 and CD28' vs 'none'
SLC35D2 DOWN in 'CD3 and CD28' vs 'none'
ATXN7L1 DOWN in 'CD3 and CD28' vs 'none'
N4BP2L1 DOWN in 'CD3 and CD28' vs 'none'
RP13-977J11.2 DOWN in 'CD3 and CD28' vs 'none'
LINC00565 DOWN in 'CD3 and CD28' vs 'none'
C6orf48 DOWN in 'CD3 and CD28' vs 'none'
NCKAP1L DOWN in 'CD3 and CD28' vs 'none'
SLC18B1 DOWN in 'CD3 and CD28' vs 'none'
CTD-3092A11.2 DOWN in 'CD3 and CD28' vs 'none'
BZRAP1-AS1 DOWN in 'CD3 and CD28' vs 'none'
SLCO3A1 DOWN in 'CD3 and CD28' vs 'none'
AC004941.5 UP in 'CD3 and CD28' vs 'none'
GADD45GIP1 UP in 'CD3 and CD28' vs 'none'
C14orf132 DOWN in 'CD3 and CD28' vs 'none'
TSC22D1 DOWN in 'CD3 and CD28' vs 'none'
CDC42SE2 DOWN in 'CD3 and CD28' vs 'none'
ANKRD12 DOWN in 'CD3 and CD28' vs 'none'
GAL3ST4 DOWN in 'CD3 and CD28' vs 'none'
(Factor value) DISEASE/systemic lupus erythematosus
TBC1D10C DOWN in 'CD3 and CD28' vs 'none'
MPHOSPH10 UP in 'CD3 and CD28' vs 'none'
FAM13A-AS1 DOWN in 'CD3 and CD28' vs 'none'
L3MBTL3 DOWN in 'CD3 and CD28' vs 'none'
(Sample) disease/systemic lupus erythematosus
CAPRIN2 DOWN in 'CD3 and CD28' vs 'none'
D4S234E DOWN in 'CD3 and CD28' vs 'none'
KANSL1-AS1 DOWN in 'CD3 and CD28' vs 'none'
ATF7IP2 DOWN in 'nitric oxide' vs 'none'
RP11-693N9.2 DOWN in 'CD3 and CD28' vs 'none'
DNAJC15 DOWN in 'CD3 and CD28' vs 'none'
AC005682.5 UP in 'CD3 and CD28' vs 'none'
RP11-658F2.8 DOWN in 'CD3 and CD28' vs 'none'
SERINC5 DOWN in 'CD3 and CD28' vs 'none'
CTC-425F1.4 UP in 'CD3 and CD28' vs 'none'
APBB1IP DOWN in 'CD3 and CD28' vs 'none'
CENPBD1P1 UP in 'CD3 and CD28' vs 'none'
TMEM154 DOWN in 'CD3 and CD28' vs 'none'
AC017002.1 UP in 'CD3 and CD28' vs 'none'
RAB11FIP3 DOWN in 'CD3 and CD28' vs 'none'
N4BP2L2 DOWN in 'CD3 and CD28' vs 'none'
NOTCH2NL DOWN in 'CD3 and CD28' vs 'none'
FLJ37453 DOWN in 'CD3 and CD28' vs 'none'
RNASET2 DOWN in 'CD3 and CD28' vs 'none'
FAM134C DOWN in 'CD3 and CD28' vs 'none'
ANKRD13D DOWN in 'CD3 and CD28' vs 'none'
GRAMD1A DOWN in 'CD3 and CD28' vs 'none'
ARHGAP12 DOWN in 'CD3 and CD28' vs 'none'
TSPAN14 DOWN in 'CD3 and CD28' vs 'none'