(Factor value) GROWTH_CONDITION/no co-culture
CLECL1 UP in 'co-culture with nurse-like cells' vs 'no co-culture'
CD180 UP in 'co-culture with nurse-like cells' vs 'no co-culture'
MAP1LC3B DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
GKAP1 DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
MAFF DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
KLF6 DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
(Sample) disease/chronic lymphocytic leukemia
(Factor value) GROWTH_CONDITION/co-culture with nurse-like cells
CHPT1 DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
EZR DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
In the marrow and lymphatic tissues, chronic lymphocytic leukemia (CLL) cells interact with accessory cells that constitute the leukemia microenvironment. In lymphatic tissues, CLL cells are interspersed with CD68+ nurselike cells (NLC) and T cells. However, the mechanism regulating co-localization of CLL cells and these accessory cells are largely unknown. To dissect the molecular cross-talk between CLL and NLC, we profiled the gene expression of CD19-purified CLL cells before and after co-culture with NLC. NLC co-culture induced high-level expression of B cell maturation antigen (BCMA) and two chemoattractants (CCL3, CCL4) by CLL cells. Supernatants from CLL-NLC co-cultures revealed high CCL3/CCL4 protein levels. B cell receptor triggering also induced a robust induction of CCL3 and CCL4 expression by CLL cells, which was almost completely abrogated by a specific Syc inhibitor, R406. High CCL3 and CCL4 plasma levels in CLL patients suggest that activation of this pathway plays a role in vivo. These studies reveal a novel mechanism of cross-talk between CLL cells and their microenvironment, namely the secretion of two T cell chemokines by CLL-NLC interaction and in response to BCR stimulation. Through these chemokines, CLL cells can recruit accessory cells, and thereby actively create a microenvironment that favors their growth and survival. Experiment Overall Design: The microarray part of this study included samples of CLL cells from 9 different patients, analyzed directly after purification and after 14 days of co-culture with Nurse like cells . Experiment Overall Design: In detail, RNA was isolated from CD19-purified CLL cells from 9 different patientsÃÂ¢ÃÂÃÂ peripheral blood mononuclear cells (PBMC) after Ficoll separation and subsequent purification with CD19 MicroBeads and the MACSÃÂÃÂ® technology according to the manufacturerÃÂ¢ÃÂÃÂs instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). For comparison, the same CLL cell samples were co-cultured for 14 days with NLC (\"\"4d NLC\"\").\"\"). For co-culture with NLC, PBMC from patients with CLL were suspended in complete RPMI medium (RPMI1640 with 10% FCS, penicillin-streptomycin-glutamine, Gibco-BRL, Grand Island, NY) to a concentration of 1 x 107/ml (total 20 ml) and incubated for 14 days in 75 cm2 tissue culture flasks (Techno Plastic Products AG) as described previously3. Nonadherent lymphoid cells then were removed and the NLC layer was washed two times with phosphate-buffered saline (PBS). The complete removal of lymphocytes was verified by phase-contrast microscopy. The nonadherent cells together with the wash-fractions were then used for RNA preparation. In order to purify the CLL B cells prior to RNA isolation, CLL PBMC were passed through a 30 ÃÂÃÂµm nylon mesh to obtain a single-cell suspension. Then CLL B cells were purified with CD19 MicroBeads. Subsequently RNA was extracted.\"
JOSD1 DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
TSC22D3 DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
RALGPS2 UP in 'co-culture with nurse-like cells' vs 'no co-culture'
Analysis of 'co-culture with nurse-like cells' vs 'no co-culture'
IRS2 DOWN in 'co-culture with nurse-like cells' vs 'no co-culture'
BCAR3 UP in 'co-culture with nurse-like cells' vs 'no co-culture'