SMIM14 DOWN in 'WT1 siRNA' vs 'control siRNA'
TTLL7 DOWN in 'WT1 siRNA' vs 'control siRNA'
TMEM2 DOWN in 'WT1 siRNA' vs 'control siRNA'
CTGF DOWN in 'WT1 siRNA' vs 'control siRNA'
COMMD7 DOWN in 'WT1 siRNA' vs 'control siRNA'
IER2 DOWN in 'WT1 siRNA' vs 'control siRNA'
CPLX1 UP in 'WT1 siRNA' vs 'control siRNA'
SLC16A6 DOWN in 'WT1 siRNA' vs 'control siRNA'
SAMHD1 UP in 'WT1 siRNA' vs 'control siRNA'
CDC42SE2 UP in 'WT1 siRNA' vs 'control siRNA'
DDAH1 UP in 'WT1 siRNA' vs 'control siRNA'
CDCA3 DOWN in 'WT1 siRNA' vs 'control siRNA'
LIPH DOWN in 'WT1 siRNA' vs 'control siRNA'
HEG1 DOWN in 'WT1 siRNA' vs 'control siRNA'
ITIH1 UP in 'WT1 siRNA' vs 'control siRNA'
PTGER4 DOWN in 'WT1 siRNA' vs 'control siRNA'
DOK4 DOWN in 'WT1 siRNA' vs 'control siRNA'
CBX5 UP in 'WT1 siRNA' vs 'control siRNA'
GCNT3 DOWN in 'WT1 siRNA' vs 'control siRNA'
TM4SF4 UP in 'WT1 siRNA' vs 'control siRNA'
RBM47 UP in 'WT1 siRNA' vs 'control siRNA'
AMFR DOWN in 'WT1 siRNA' vs 'control siRNA'
UBASH3B DOWN in 'WT1 siRNA' vs 'control siRNA'
ID1 DOWN in 'WT1 siRNA' vs 'control siRNA'
CA9 DOWN in 'WT1 siRNA' vs 'control siRNA'
TTPA UP in 'WT1 siRNA' vs 'control siRNA'
SLC1A6 DOWN in 'WT1 siRNA' vs 'control siRNA'
RALGPS2 UP in 'WT1 siRNA' vs 'control siRNA'
PEG10 UP in 'WT1 siRNA' vs 'control siRNA'
DUSP6 DOWN in 'WT1 siRNA' vs 'control siRNA'
TTR UP in 'WT1 siRNA' vs 'control siRNA'
NUP98 UP in 'WT1 siRNA' vs 'control siRNA'
DSCAM-AS1 DOWN in 'WT1 siRNA' vs 'control siRNA'
SPRY4 DOWN in 'WT1 siRNA' vs 'control siRNA'
SLC30A10 UP in 'WT1 siRNA' vs 'control siRNA'
TMEM198 UP in 'WT1 siRNA' vs 'control siRNA'
HEY1 DOWN in 'WT1 siRNA' vs 'control siRNA'
IL18 DOWN in 'WT1 siRNA' vs 'control siRNA'
ZMAT3 DOWN in 'WT1 siRNA' vs 'control siRNA'
SLC7A11 UP in 'WT1 siRNA' vs 'control siRNA'
PPP2R1B UP in 'WT1 siRNA' vs 'control siRNA'
TMEM161B DOWN in 'WT1 siRNA' vs 'control siRNA'
LIPA DOWN in 'WT1 siRNA' vs 'control siRNA'
TMEM136 DOWN in 'WT1 siRNA' vs 'control siRNA'
PCTP UP in 'WT1 siRNA' vs 'control siRNA'
TGOLN2 DOWN in 'WT1 siRNA' vs 'control siRNA'
SGK1 DOWN in 'WT1 siRNA' vs 'control siRNA'
LINC00467 DOWN in 'WT1 siRNA' vs 'control siRNA'
EIF4E2 DOWN in 'WT1 siRNA' vs 'control siRNA'
RNF144A UP in 'WT1 siRNA' vs 'control siRNA'
PAPSS2 UP in 'WT1 siRNA' vs 'control siRNA'
CEMIP DOWN in 'WT1 siRNA' vs 'control siRNA'
TTC14 UP in 'WT1 siRNA' vs 'control siRNA'
CHGB UP in 'WT1 siRNA' vs 'control siRNA'
C9orf91 UP in 'WT1 siRNA' vs 'control siRNA'
MIPEP UP in 'WT1 siRNA' vs 'control siRNA'
PAQR8 DOWN in 'WT1 siRNA' vs 'control siRNA'
VEZT DOWN in 'WT1 siRNA' vs 'control siRNA'
STMN3 UP in 'WT1 siRNA' vs 'control siRNA'
REST DOWN in 'WT1 siRNA' vs 'control siRNA'
(Factor value) RNA_INTERFERENCE/WT1 siRNA
RP11-195F19.9 DOWN in 'WT1 siRNA' vs 'control siRNA'
GDF15 DOWN in 'WT1 siRNA' vs 'control siRNA'
G6PC UP in 'WT1 siRNA' vs 'control siRNA'
STK3 DOWN in 'WT1 siRNA' vs 'control siRNA'
ETV5 DOWN in 'WT1 siRNA' vs 'control siRNA'
FRS2 DOWN in 'WT1 siRNA' vs 'control siRNA'
PERP DOWN in 'WT1 siRNA' vs 'control siRNA'
FAM169A DOWN in 'WT1 siRNA' vs 'control siRNA'
ELOVL6 DOWN in 'WT1 siRNA' vs 'control siRNA'
HCG11 DOWN in 'WT1 siRNA' vs 'control siRNA'
H19 DOWN in 'WT1 siRNA' vs 'control siRNA'
NADK2 UP in 'WT1 siRNA' vs 'control siRNA'
HINT3 DOWN in 'WT1 siRNA' vs 'control siRNA'
APOH UP in 'WT1 siRNA' vs 'control siRNA'
AURKA DOWN in 'WT1 siRNA' vs 'control siRNA'
PROS1 DOWN in 'WT1 siRNA' vs 'control siRNA'
WT1 DOWN in 'WT1 siRNA' vs 'control siRNA'
MAFK DOWN in 'WT1 siRNA' vs 'control siRNA'
CCL20 DOWN in 'WT1 siRNA' vs 'control siRNA'
ACTL6B UP in 'WT1 siRNA' vs 'control siRNA'
USP25 DOWN in 'WT1 siRNA' vs 'control siRNA'
RBBP9 UP in 'WT1 siRNA' vs 'control siRNA'
NR4A2 DOWN in 'WT1 siRNA' vs 'control siRNA'
WRAP73 DOWN in 'WT1 siRNA' vs 'control siRNA'
ONECUT2 UP in 'WT1 siRNA' vs 'control siRNA'
MFSD9 DOWN in 'WT1 siRNA' vs 'control siRNA'
LINC00511 DOWN in 'WT1 siRNA' vs 'control siRNA'
RP4-639F20.1 DOWN in 'WT1 siRNA' vs 'control siRNA'
LINC00473 DOWN in 'WT1 siRNA' vs 'control siRNA'
FAM120AOS DOWN in 'WT1 siRNA' vs 'control siRNA'
MIR7-3HG UP in 'WT1 siRNA' vs 'control siRNA'
CCNG2 DOWN in 'WT1 siRNA' vs 'control siRNA'
SERPIND1 UP in 'WT1 siRNA' vs 'control siRNA'
SLC25A12 DOWN in 'WT1 siRNA' vs 'control siRNA'
IER3 DOWN in 'WT1 siRNA' vs 'control siRNA'
LAMTOR5-AS1 DOWN in 'WT1 siRNA' vs 'control siRNA'
S100P DOWN in 'WT1 siRNA' vs 'control siRNA'
EGR1 DOWN in 'WT1 siRNA' vs 'control siRNA'
SCGN UP in 'WT1 siRNA' vs 'control siRNA'
C3orf52 DOWN in 'WT1 siRNA' vs 'control siRNA'
DNMT3B DOWN in 'WT1 siRNA' vs 'control siRNA'
SLC44A1 UP in 'WT1 siRNA' vs 'control siRNA'
VAV3 DOWN in 'WT1 siRNA' vs 'control siRNA'
FAM206A DOWN in 'WT1 siRNA' vs 'control siRNA'
ANKRD37 DOWN in 'WT1 siRNA' vs 'control siRNA'
The Wilms' tumor 1 gene (WT1) encodes a transcription factor involved in cell growth and development. As we previously reported WT1 expression is hardly detectable in normal hepatic tissue but is induced in liver cirrhosis. Although WT1 has been found to be overexpressed in a number of malignancies, the role of WT1 in hepatocarcinogenesis has not been clarified. We found that WT1 is expressed in several human hepatocellular carcinoma (HCC) cell lines including PLC/PRF/5 and HepG2, and in HCC tumor tissue in 42% of patients. WT1 small interfering RNAs did not affect proliferation rate of HCC cells but abrogated their resistance to anoikis. Transcriptome analysis of PLC/PRF/5 cells after WT1 knockdown demonstrated upregulation of 251 genes and downregulation of 321. Ninety per cent of the former corresponded to metabolic genes mostly those characterizing the mature hepatocyte phenotype. On the contrary, genes that decreased upon WT1 inhibition were mainly related to defense against apoptosis, cell cycle and tumor progression. In agreement with these findings WT1 expression increased the resistance of liver tumor cells to doxorubicin, a compound used to treat HCC. Interestingly, doxorubicin strongly enhanced WT1 expression in both HCC cells and normal human hepatocytes. Among different chemotherapeutics, induction of WT1 transcription was restricted to topoisomerase 2 inhibitors. When WT1 expression was prohibited doxorubicin caused a marked increase in caspase-3 activation. In conclusion, WT1 is expressed in a substantial proportion of HCC contributing to tumor progression and resistance to chemotherapy, suggesting that WT1 may be an important target for HCC treatment.
SERINC3 DOWN in 'WT1 siRNA' vs 'control siRNA'
RBMS1 DOWN in 'WT1 siRNA' vs 'control siRNA'
SEC63 DOWN in 'WT1 siRNA' vs 'control siRNA'
SCG3 UP in 'WT1 siRNA' vs 'control siRNA'
SULT2A1 UP in 'WT1 siRNA' vs 'control siRNA'
STARD4 DOWN in 'WT1 siRNA' vs 'control siRNA'
SECISBP2 DOWN in 'WT1 siRNA' vs 'control siRNA'
HPX UP in 'WT1 siRNA' vs 'control siRNA'
ARL6IP5 UP in 'WT1 siRNA' vs 'control siRNA'
PAK2 DOWN in 'WT1 siRNA' vs 'control siRNA'
GJA1 DOWN in 'WT1 siRNA' vs 'control siRNA'
RAB11FIP4 UP in 'WT1 siRNA' vs 'control siRNA'
ETV4 DOWN in 'WT1 siRNA' vs 'control siRNA'
PHTF2 DOWN in 'WT1 siRNA' vs 'control siRNA'
DIXDC1 DOWN in 'WT1 siRNA' vs 'control siRNA'
AKR1D1 UP in 'WT1 siRNA' vs 'control siRNA'
C8orf4 DOWN in 'WT1 siRNA' vs 'control siRNA'
CTSL DOWN in 'WT1 siRNA' vs 'control siRNA'
NNMT DOWN in 'WT1 siRNA' vs 'control siRNA'
ETV1 DOWN in 'WT1 siRNA' vs 'control siRNA'
C4orf46 UP in 'WT1 siRNA' vs 'control siRNA'
ALB UP in 'WT1 siRNA' vs 'control siRNA'
RASD1 DOWN in 'WT1 siRNA' vs 'control siRNA'
RDH13 UP in 'WT1 siRNA' vs 'control siRNA'
TFPI DOWN in 'WT1 siRNA' vs 'control siRNA'
PTGFRN DOWN in 'WT1 siRNA' vs 'control siRNA'
PPP6C DOWN in 'WT1 siRNA' vs 'control siRNA'
IGSF3 DOWN in 'WT1 siRNA' vs 'control siRNA'
SUGT1 UP in 'WT1 siRNA' vs 'control siRNA'
STX6 DOWN in 'WT1 siRNA' vs 'control siRNA'
KCTD10 DOWN in 'WT1 siRNA' vs 'control siRNA'
FAM13B UP in 'WT1 siRNA' vs 'control siRNA'
PANK1 UP in 'WT1 siRNA' vs 'control siRNA'
(Factor value) RNA_INTERFERENCE/control siRNA
RRM2 DOWN in 'WT1 siRNA' vs 'control siRNA'
BAAT UP in 'WT1 siRNA' vs 'control siRNA'
PTPMT1 DOWN in 'WT1 siRNA' vs 'control siRNA'
HPGD UP in 'WT1 siRNA' vs 'control siRNA'
Analysis of 'WT1 siRNA' vs 'control siRNA'
DIO1 UP in 'WT1 siRNA' vs 'control siRNA'
HMGCS2 UP in 'WT1 siRNA' vs 'control siRNA'
GDAP1 UP in 'WT1 siRNA' vs 'control siRNA'
BDH1 UP in 'WT1 siRNA' vs 'control siRNA'
LHFPL2 DOWN in 'WT1 siRNA' vs 'control siRNA'
ELOVL1 DOWN in 'WT1 siRNA' vs 'control siRNA'
AP000251.3 DOWN in 'WT1 siRNA' vs 'control siRNA'
NR4A1 DOWN in 'WT1 siRNA' vs 'control siRNA'
BHLHE40 DOWN in 'WT1 siRNA' vs 'control siRNA'
ANPEP UP in 'WT1 siRNA' vs 'control siRNA'
C16orf45 DOWN in 'WT1 siRNA' vs 'control siRNA'
GATM UP in 'WT1 siRNA' vs 'control siRNA'
NLRP1 DOWN in 'WT1 siRNA' vs 'control siRNA'
CXCL5 DOWN in 'WT1 siRNA' vs 'control siRNA'
LINC00312 DOWN in 'WT1 siRNA' vs 'control siRNA'
CGA DOWN in 'WT1 siRNA' vs 'control siRNA'
GOLT1B DOWN in 'WT1 siRNA' vs 'control siRNA'
SLC25A18 DOWN in 'WT1 siRNA' vs 'control siRNA'
PAQR9 UP in 'WT1 siRNA' vs 'control siRNA'
SLC46A1 UP in 'WT1 siRNA' vs 'control siRNA'
RP11-111M22.3 DOWN in 'WT1 siRNA' vs 'control siRNA'
C17orf58 DOWN in 'WT1 siRNA' vs 'control siRNA'
SLC51B DOWN in 'WT1 siRNA' vs 'control siRNA'
RP11-758H9.2 DOWN in 'WT1 siRNA' vs 'control siRNA'
ADH1A UP in 'WT1 siRNA' vs 'control siRNA'
TOMM20 DOWN in 'WT1 siRNA' vs 'control siRNA'