CG9512 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG18607 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG10562 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG7433 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG5246 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG18606 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CaBP1 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Ugt37b1 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG5009 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG11893 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG5011 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Aldh-III DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG11912 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
pdgy DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG15068 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Ugt86Da DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG15773 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG5999 UP in 'Bowman-Birk Inhibitor' vs 'normal'
mag DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG5724 UP in 'Bowman-Birk Inhibitor' vs 'normal'
cact UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG8788 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Paip2 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG14688 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
(Sample) developmental stage/3rd instar larva
CG13488 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG11162 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG4729 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Adgf-A DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Bace UP in 'Bowman-Birk Inhibitor' vs 'normal'
Cyp4d1 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Ero1L UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG9547 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG4362 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG9396 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Cyp4d14 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
alpha-Est4 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Rel UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG6048 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG42365 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG9555 UP in 'Bowman-Birk Inhibitor' vs 'normal'
P58IPK UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG5892 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Thor UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG1544 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
alpha-Est2 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG31089 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Mal-A6 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG14606 UP in 'Bowman-Birk Inhibitor' vs 'normal'
l(2)k09913 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG2915 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6574 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG9465 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG12262 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG15533 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG34284 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Dpt UP in 'Bowman-Birk Inhibitor' vs 'normal'
Jon99Ci DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
(Factor value) DIET/Bowman-Birk Inhibitor
(Assay) D.m.Third_instar_larval_midgut_normal_12h_rep3
CG15784 UP in 'Bowman-Birk Inhibitor' vs 'normal'
vanin-like UP in 'Bowman-Birk Inhibitor' vs 'normal'
Mal-A3 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Amyrel DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
yip2 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG11899 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
sel UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG7298 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Sodh-1 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG13101 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Hexo1 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG5002 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG15155 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Mal-A1 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6287 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6996 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG10420 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG12868 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG9259 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG10621 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Akap200 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Bowman-Birk Inhibitor (BBI) has both insecticidal and anti-cancerous properties. It has been hypothesized that dietary BBI slows insect growth by inhibiting the catalytic activity of digestive enzymes trypsins and chyomotrypsins, resulting in the midgut having reduced access to amino acids needed for growth. In mammals, BBI was hypothesized to influence cellular energy metabolism. Thus, we tested the hypothesis that dietary BBI also impacts energy-associated pathways in the midgut of Drosophila melanogaster. We investigated the impact of dietary BBI on the following parameters in the midguts of third-instar Drosophila larvae: (i) cellular metabolites, (ii) global transcriptome response, (iii) putative transcription factor binding sites (TFBSs) associated with the differentially expressed transcripts, and (iv) epithelial cellular structure. Dietary BBI caused: (i) a reduction of cellular DHAP, glucose, and succinate; and, (ii) increased Fructuse-6-phosphate; (ii) differential expression of genes associated with the glucose and fatty acid utilization; and, (iii) a shortening of midgut epithelial microvilli, a phenomenon previously associated with glucose starvation. Additionally, fifty seven percent of the putative TFBSs associated with the differentially expressed transcripts have previously been associated with glucose and insulin activities in mammalian studies. Collectively these results support the hypothesis that dietary BBI influences energy utilization in the Drosophila midgut. Experiment Overall Design: Two treatments (control vs BBI-fed), and three replicates were conducted. There were total six samples. There was no dye-swap.
CG31104 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG16775 UP in 'Bowman-Birk Inhibitor' vs 'normal'
(Assay) D.m.Third_instar_larval_midgut_normal_12h_rep1
CG15126 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG3106 UP in 'Bowman-Birk Inhibitor' vs 'normal'
smp-30 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6178 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG13659 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG5059 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG17147 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG8468 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6903 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG9509 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG11208 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
su(r) DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG7966 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG10184 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG6295 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6084 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Npc1b DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
(Sample) organism/Drosophila melanogaster
BI-1 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG6484 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG9498 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG5550 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG9706 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Mipp1 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Acox57D-p DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6126 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Dip-C DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG10960 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG2254 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Npc2f UP in 'Bowman-Birk Inhibitor' vs 'normal'
Analysis of 'Bowman-Birk Inhibitor' vs 'normal'
CG4363 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG18179 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Jon66Cii DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG7582 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Jon99Fi DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Fbp2 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6834 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG6271 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG3699 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG3348 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG5804 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
GstD2 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Cyp6d5 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG7381 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG17570 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Cyp6a17 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Ssadh DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG2004 UP in 'Bowman-Birk Inhibitor' vs 'normal'
GstD9 UP in 'Bowman-Birk Inhibitor' vs 'normal'
PGRP-SC1b DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
(Assay) D.m.Third_instar_larval_midgut_normal_12h_rep2
(Assay) Third_instar_larval_midgut_BBI_12h_rep1
thetaTry DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
UK114 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
ScpX DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG17337 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Manf UP in 'Bowman-Birk Inhibitor' vs 'normal'
Fatp DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Est-Q DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG3292 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Cyp4ac3 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Cyp28d2 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
(Assay) Third_instar_larval_midgut_BBI_12h_rep2
CG4835 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Jheh2 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG17597 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG33012 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG31148 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG10911 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG7460 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
NUCB1 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG31075 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Cht9 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Cyp28a5 UP in 'Bowman-Birk Inhibitor' vs 'normal'
Scp2 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
(Assay) Third_instar_larval_midgut_BBI_12h_rep3
CG9825 UP in 'Bowman-Birk Inhibitor' vs 'normal'
LpR1 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG9463 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG6277 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG8664 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
Cyt-b5-r DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG1718 DOWN in 'Bowman-Birk Inhibitor' vs 'normal'
CG15043 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG6115 UP in 'Bowman-Birk Inhibitor' vs 'normal'
CG9449 UP in 'Bowman-Birk Inhibitor' vs 'normal'