HRG DOWN in 'Tmprss6 wild type' vs 'empty vector'
TXNIP UP in 'Tmprss6 mutant' vs 'empty vector'
PRRG4 DOWN in 'Tmprss6 mutant' vs 'empty vector'
EHF DOWN in 'Tmprss6 wild type' vs 'empty vector'
HPGD DOWN in 'Tmprss6 mutant' vs 'empty vector'
SLC4A3 DOWN in 'Tmprss6 wild type' vs 'empty vector'
ELF5 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ATP8B4 DOWN in 'Tmprss6 wild type' vs 'empty vector'
MCHR1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
FGA UP in 'Tmprss6 mutant' vs 'empty vector'
IL11 UP in 'Tmprss6 wild type' vs 'empty vector'
RASSF8 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SPATA16 DOWN in 'Tmprss6 wild type' vs 'empty vector'
Analysis of 'Tmprss6 wild type' vs 'empty vector'
RHOJ DOWN in 'Tmprss6 mutant' vs 'empty vector'
KRT23 UP in 'Tmprss6 wild type' vs 'empty vector'
(Factor value) GENOTYPE/Tmprss6 wild type
NFIB DOWN in 'Tmprss6 wild type' vs 'empty vector'
AKAP12 UP in 'Tmprss6 mutant' vs 'empty vector'
SOCS2 UP in 'Tmprss6 wild type' vs 'empty vector'
RP11-295M3.4 DOWN in 'Tmprss6 wild type' vs 'empty vector'
GPRC5A UP in 'Tmprss6 wild type' vs 'empty vector'
TNFSF14 UP in 'Tmprss6 mutant' vs 'empty vector'
HIST1H2BD DOWN in 'Tmprss6 mutant' vs 'empty vector'
ARHGAP42 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SLC7A10 DOWN in 'Tmprss6 mutant' vs 'empty vector'
PAPPA DOWN in 'Tmprss6 wild type' vs 'empty vector'
IGFBP1 UP in 'Tmprss6 wild type' vs 'empty vector'
SOX4 UP in 'Tmprss6 wild type' vs 'empty vector'
PCSK5 DOWN in 'Tmprss6 mutant' vs 'empty vector'
LINC00973 UP in 'Tmprss6 mutant' vs 'empty vector'
LRBA DOWN in 'Tmprss6 mutant' vs 'empty vector'
CXCL8 UP in 'Tmprss6 wild type' vs 'empty vector'
ANXA13 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ZNF320 DOWN in 'Tmprss6 wild type' vs 'empty vector'
LRBA DOWN in 'Tmprss6 wild type' vs 'empty vector'
PRRG4 DOWN in 'Tmprss6 wild type' vs 'empty vector'
SOX7 DOWN in 'Tmprss6 mutant' vs 'empty vector'
DCLRE1B DOWN in 'Tmprss6 wild type' vs 'empty vector'
(Sample) genetic modification/transfection
MYLK4 DOWN in 'Tmprss6 wild type' vs 'empty vector'
AKAP12 UP in 'Tmprss6 wild type' vs 'empty vector'
RHOJ DOWN in 'Tmprss6 wild type' vs 'empty vector'
FAM167B DOWN in 'Tmprss6 mutant' vs 'empty vector'
MYZAP DOWN in 'Tmprss6 mutant' vs 'empty vector'
TSC22D3 DOWN in 'Tmprss6 mutant' vs 'empty vector'
FGA UP in 'Tmprss6 wild type' vs 'empty vector'
SOCS2 UP in 'Tmprss6 mutant' vs 'empty vector'
KRT23 UP in 'Tmprss6 mutant' vs 'empty vector'
ANXA13 DOWN in 'Tmprss6 wild type' vs 'empty vector'
OSBPL1A DOWN in 'Tmprss6 wild type' vs 'empty vector'
REPS2 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ATP8A1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
ANG UP in 'Tmprss6 mutant' vs 'empty vector'
KRTAP2-3 UP in 'Tmprss6 wild type' vs 'empty vector'
NR5A2 DOWN in 'Tmprss6 wild type' vs 'empty vector'
RASSF8 DOWN in 'Tmprss6 wild type' vs 'empty vector'
PDK3 DOWN in 'Tmprss6 mutant' vs 'empty vector'
EDNRB DOWN in 'Tmprss6 wild type' vs 'empty vector'
HRG DOWN in 'Tmprss6 mutant' vs 'empty vector'
LCOR DOWN in 'Tmprss6 wild type' vs 'empty vector'
SEMA4D DOWN in 'Tmprss6 wild type' vs 'empty vector'
MYLK4 DOWN in 'Tmprss6 mutant' vs 'empty vector'
EDNRB DOWN in 'Tmprss6 mutant' vs 'empty vector'
PCSK5 DOWN in 'Tmprss6 wild type' vs 'empty vector'
NR5A2 DOWN in 'Tmprss6 mutant' vs 'empty vector'
PDK3 DOWN in 'Tmprss6 wild type' vs 'empty vector'
ENDOD1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ALDH8A1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
LCP1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
LCOR DOWN in 'Tmprss6 mutant' vs 'empty vector'
UCP1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
TIMP4 DOWN in 'Tmprss6 wild type' vs 'empty vector'
GS1-358P8.4 DOWN in 'Tmprss6 mutant' vs 'empty vector'
EHF DOWN in 'Tmprss6 mutant' vs 'empty vector'
CP UP in 'Tmprss6 mutant' vs 'empty vector'
PLAU DOWN in 'Tmprss6 mutant' vs 'empty vector'
UCP1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SOWAHA DOWN in 'Tmprss6 mutant' vs 'empty vector'
CAB39L DOWN in 'Tmprss6 mutant' vs 'empty vector'
GC DOWN in 'Tmprss6 mutant' vs 'empty vector'
AGR2 UP in 'Tmprss6 wild type' vs 'empty vector'
CYR61 UP in 'Tmprss6 wild type' vs 'empty vector'
PAPPA DOWN in 'Tmprss6 mutant' vs 'empty vector'
ALDH8A1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
LCP1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
GLS DOWN in 'Tmprss6 wild type' vs 'empty vector'
TPD52L1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
KRTAP3-1 UP in 'Tmprss6 wild type' vs 'empty vector'
ABCA5 DOWN in 'Tmprss6 wild type' vs 'empty vector'
FAM167B DOWN in 'Tmprss6 wild type' vs 'empty vector'
SOX5 DOWN in 'Tmprss6 wild type' vs 'empty vector'
NUAK1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
PTGS1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
ITIH5 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SPATA16 DOWN in 'Tmprss6 mutant' vs 'empty vector'
AREG UP in 'Tmprss6 wild type' vs 'empty vector'
CDH16 DOWN in 'Tmprss6 mutant' vs 'empty vector'
S1PR3 DOWN in 'Tmprss6 mutant' vs 'empty vector'
AXL UP in 'Tmprss6 wild type' vs 'empty vector'
TXNIP UP in 'Tmprss6 wild type' vs 'empty vector'
SLC4A11 DOWN in 'Tmprss6 mutant' vs 'empty vector'
DAB1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
DYRK3 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SLC22A16 DOWN in 'Tmprss6 mutant' vs 'empty vector'
GBP2 DOWN in 'Tmprss6 wild type' vs 'empty vector'
MCHR1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ITIH5 DOWN in 'Tmprss6 wild type' vs 'empty vector'
CYP3A5 DOWN in 'Tmprss6 mutant' vs 'empty vector'
GLS DOWN in 'Tmprss6 mutant' vs 'empty vector'
ATP8B4 DOWN in 'Tmprss6 mutant' vs 'empty vector'
KCNK3 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ATP8A1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
DYRK3 DOWN in 'Tmprss6 wild type' vs 'empty vector'
CYP3A7 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SOX7 DOWN in 'Tmprss6 wild type' vs 'empty vector'
NADK2 DOWN in 'Tmprss6 mutant' vs 'empty vector'
DCLRE1B DOWN in 'Tmprss6 mutant' vs 'empty vector'
MATN2 DOWN in 'Tmprss6 wild type' vs 'empty vector'
CYP3A4 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SVIP DOWN in 'Tmprss6 mutant' vs 'empty vector'
SEMA4D DOWN in 'Tmprss6 mutant' vs 'empty vector'
C7 DOWN in 'Tmprss6 wild type' vs 'empty vector'
SLC6A12 DOWN in 'Tmprss6 mutant' vs 'empty vector'
IER5 DOWN in 'Tmprss6 mutant' vs 'empty vector'
TMPRSS6 UP in 'Tmprss6 wild type' vs 'empty vector'
CAPN8 DOWN in 'Tmprss6 mutant' vs 'empty vector'
PLSCR4 UP in 'Tmprss6 mutant' vs 'empty vector'
RP11-295M3.4 DOWN in 'Tmprss6 mutant' vs 'empty vector'
GC DOWN in 'Tmprss6 wild type' vs 'empty vector'
OSBPL1A DOWN in 'Tmprss6 mutant' vs 'empty vector'
TMPRSS6 is a type II transmembrane serine protease and is revealed by our work to be part of a low-iron sensing pathway. When animal gets iron deficient, TMPRSS6 is required to shut off hepcidin gene, so as to allow iron to be uptaken from GI tract. The mutant mouse, which was generated by ENU mutagenesis, has developed microcytic anemia. The phenotype is caused by a splicing error in Tmprss6 gene. However, the mechanism of TMPRSS6 effect remains elusive. To gain further insight into the molecular components of the TMPRSS6 signaling pathway, we overexpressed either TMPRSS6 or its mutant version of protein in human liver carcinoma cell line HepG2 cells, and compared the transcription status betweem these two treatments. Experiment Overall Design: Triplicate per transfection with wildtype Tmprss6 cDNA, mutant version of Tmprss6 cDNA and empty vector as control, respectively. 36 hours post transfection, cells were harvested and subjected to RNA extraction.
CLCN4 DOWN in 'Tmprss6 mutant' vs 'empty vector'
TPD52L1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
GBP2 DOWN in 'Tmprss6 mutant' vs 'empty vector'
TSC22D3 DOWN in 'Tmprss6 wild type' vs 'empty vector'
PTGS1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ARRDC3 UP in 'Tmprss6 wild type' vs 'empty vector'
THBS1 UP in 'Tmprss6 mutant' vs 'empty vector'
GULP1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
KCNK3 DOWN in 'Tmprss6 wild type' vs 'empty vector'
CEP55 DOWN in 'Tmprss6 mutant' vs 'empty vector'
REPS2 DOWN in 'Tmprss6 wild type' vs 'empty vector'
AQP3 DOWN in 'Tmprss6 mutant' vs 'empty vector'
DUSP5 UP in 'Tmprss6 wild type' vs 'empty vector'
HPGD DOWN in 'Tmprss6 wild type' vs 'empty vector'
TMEM27 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ANKRD1 UP in 'Tmprss6 wild type' vs 'empty vector'
TAGLN UP in 'Tmprss6 wild type' vs 'empty vector'
SYNE1 UP in 'Tmprss6 wild type' vs 'empty vector'
NFIB DOWN in 'Tmprss6 mutant' vs 'empty vector'
RAMP1 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SSTR2 DOWN in 'Tmprss6 mutant' vs 'empty vector'
MATN2 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ETNPPL DOWN in 'Tmprss6 wild type' vs 'empty vector'
ETNPPL DOWN in 'Tmprss6 mutant' vs 'empty vector'
LINC00973 UP in 'Tmprss6 wild type' vs 'empty vector'
IL1RN UP in 'Tmprss6 mutant' vs 'empty vector'
SOX4 UP in 'Tmprss6 mutant' vs 'empty vector'
RMDN2 DOWN in 'Tmprss6 mutant' vs 'empty vector'
HSPB8 DOWN in 'Tmprss6 mutant' vs 'empty vector'
RRAD DOWN in 'Tmprss6 mutant' vs 'empty vector'
SLC4A3 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SOX5 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SLC5A3 DOWN in 'Tmprss6 mutant' vs 'empty vector'
SLC22A16 DOWN in 'Tmprss6 wild type' vs 'empty vector'
MRPS6 DOWN in 'Tmprss6 mutant' vs 'empty vector'
NEXN UP in 'Tmprss6 wild type' vs 'empty vector'
AREG UP in 'Tmprss6 mutant' vs 'empty vector'
CPLX2 UP in 'Tmprss6 mutant' vs 'empty vector'
IGFBP1 UP in 'Tmprss6 mutant' vs 'empty vector'
CRYAB DOWN in 'Tmprss6 mutant' vs 'empty vector'
MAMDC2 DOWN in 'Tmprss6 mutant' vs 'empty vector'
CDR2L DOWN in 'Tmprss6 mutant' vs 'empty vector'
ZNF320 DOWN in 'Tmprss6 mutant' vs 'empty vector'
RMDN2 DOWN in 'Tmprss6 wild type' vs 'empty vector'
RAMP1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
ABCA5 DOWN in 'Tmprss6 mutant' vs 'empty vector'
MICAL2 UP in 'Tmprss6 wild type' vs 'empty vector'
TMPRSS6 UP in 'Tmprss6 mutant' vs 'empty vector'
LRRC40 DOWN in 'Tmprss6 mutant' vs 'empty vector'
ARHGAP42 DOWN in 'Tmprss6 wild type' vs 'empty vector'
ANKRD1 UP in 'Tmprss6 mutant' vs 'empty vector'
Analysis of 'Tmprss6 mutant' vs 'empty vector'
TIMP4 DOWN in 'Tmprss6 mutant' vs 'empty vector'
C5 UP in 'Tmprss6 mutant' vs 'empty vector'
SPP1 UP in 'Tmprss6 mutant' vs 'empty vector'
GULP1 DOWN in 'Tmprss6 wild type' vs 'empty vector'
DAB2 UP in 'Tmprss6 mutant' vs 'empty vector'
CTGF UP in 'Tmprss6 mutant' vs 'empty vector'
LRRC40 DOWN in 'Tmprss6 wild type' vs 'empty vector'
CEP55 DOWN in 'Tmprss6 wild type' vs 'empty vector'
C7 DOWN in 'Tmprss6 mutant' vs 'empty vector'
MYZAP DOWN in 'Tmprss6 wild type' vs 'empty vector'
AGR2 UP in 'Tmprss6 mutant' vs 'empty vector'
EDN1 UP in 'Tmprss6 wild type' vs 'empty vector'